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The Diagnostic Utility of Natural Killer Cell Subsets in Deep Vein Thrombosis
BACKGROUND: Natural killer (NK) cells are important components of adaptive and innate immune responses. NK cell subsets have different functions and may play a role in vascular disorders. This study aimed to evaluate the proportions of NK cells and their subsets to determine whether they can be used...
Autores principales: | , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Dove
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10680478/ https://www.ncbi.nlm.nih.gov/pubmed/38025520 http://dx.doi.org/10.2147/VHRM.S430466 |
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author | Alhabibi, Alshaymaa M Wahab, Maisa A Sakr, Ahmed Khairy Abd El-Hamid, Samar M Zakaria, Mohamed Yahia Althoqapy, Azza Ali El Sayed, Hanaa Mohammed Eid Kasim, Sammar Ahmed Ibrahim, Hanan F Saleh, Ola I Ahmed, Howida A Sayed, Alaa H Lotfy, Mahmoud |
author_facet | Alhabibi, Alshaymaa M Wahab, Maisa A Sakr, Ahmed Khairy Abd El-Hamid, Samar M Zakaria, Mohamed Yahia Althoqapy, Azza Ali El Sayed, Hanaa Mohammed Eid Kasim, Sammar Ahmed Ibrahim, Hanan F Saleh, Ola I Ahmed, Howida A Sayed, Alaa H Lotfy, Mahmoud |
author_sort | Alhabibi, Alshaymaa M |
collection | PubMed |
description | BACKGROUND: Natural killer (NK) cells are important components of adaptive and innate immune responses. NK cell subsets have different functions and may play a role in vascular disorders. This study aimed to evaluate the proportions of NK cells and their subsets to determine whether they can be used as markers of venous thrombosis and to identify whether there was a link between NK cell proportion and citrullinated histone (H3) levels. PATIENTS AND METHODS: This study included 100 participants divided into Group I (n=50, patients with deep venous thrombosis (DVT)) and Group II (n=50, age- and sex-matched healthy controls). Group I was further categorized into Group Ia (n=25, patients with acute DVT) and Group Ib (n=25, patients with chronic DVT). The proportions of NK cells and their subsets were evaluated by flow cytometry using CD3/CD16/CD56. The levels of citrullinated histones (H3) were estimated using enzyme-linked immunosorbent assay (ELISA). RESULTS: Compared to the control group, DVT patients had a significantly lower proportion of (CD56 dim/CD16+) NK cells, a significantly higher proportion of (CD56-/CD16+) NK cells and a high level of citrullinated histone (H3). CONCLUSION: NK cell subsets and citrullinated histone (H3) could be used as markers for DVT and as targets for therapeutic drugs to inhibit the formation or progression of thrombosis. |
format | Online Article Text |
id | pubmed-10680478 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Dove |
record_format | MEDLINE/PubMed |
spelling | pubmed-106804782023-11-23 The Diagnostic Utility of Natural Killer Cell Subsets in Deep Vein Thrombosis Alhabibi, Alshaymaa M Wahab, Maisa A Sakr, Ahmed Khairy Abd El-Hamid, Samar M Zakaria, Mohamed Yahia Althoqapy, Azza Ali El Sayed, Hanaa Mohammed Eid Kasim, Sammar Ahmed Ibrahim, Hanan F Saleh, Ola I Ahmed, Howida A Sayed, Alaa H Lotfy, Mahmoud Vasc Health Risk Manag Original Research BACKGROUND: Natural killer (NK) cells are important components of adaptive and innate immune responses. NK cell subsets have different functions and may play a role in vascular disorders. This study aimed to evaluate the proportions of NK cells and their subsets to determine whether they can be used as markers of venous thrombosis and to identify whether there was a link between NK cell proportion and citrullinated histone (H3) levels. PATIENTS AND METHODS: This study included 100 participants divided into Group I (n=50, patients with deep venous thrombosis (DVT)) and Group II (n=50, age- and sex-matched healthy controls). Group I was further categorized into Group Ia (n=25, patients with acute DVT) and Group Ib (n=25, patients with chronic DVT). The proportions of NK cells and their subsets were evaluated by flow cytometry using CD3/CD16/CD56. The levels of citrullinated histones (H3) were estimated using enzyme-linked immunosorbent assay (ELISA). RESULTS: Compared to the control group, DVT patients had a significantly lower proportion of (CD56 dim/CD16+) NK cells, a significantly higher proportion of (CD56-/CD16+) NK cells and a high level of citrullinated histone (H3). CONCLUSION: NK cell subsets and citrullinated histone (H3) could be used as markers for DVT and as targets for therapeutic drugs to inhibit the formation or progression of thrombosis. Dove 2023-11-23 /pmc/articles/PMC10680478/ /pubmed/38025520 http://dx.doi.org/10.2147/VHRM.S430466 Text en © 2023 Alhabibi et al. https://creativecommons.org/licenses/by-nc/3.0/This work is published and licensed by Dove Medical Press Limited. The full terms of this license are available at https://www.dovepress.com/terms.php and incorporate the Creative Commons Attribution – Non Commercial (unported, v3.0) License (http://creativecommons.org/licenses/by-nc/3.0/ (https://creativecommons.org/licenses/by-nc/3.0/) ). By accessing the work you hereby accept the Terms. Non-commercial uses of the work are permitted without any further permission from Dove Medical Press Limited, provided the work is properly attributed. For permission for commercial use of this work, please see paragraphs 4.2 and 5 of our Terms (https://www.dovepress.com/terms.php). |
spellingShingle | Original Research Alhabibi, Alshaymaa M Wahab, Maisa A Sakr, Ahmed Khairy Abd El-Hamid, Samar M Zakaria, Mohamed Yahia Althoqapy, Azza Ali El Sayed, Hanaa Mohammed Eid Kasim, Sammar Ahmed Ibrahim, Hanan F Saleh, Ola I Ahmed, Howida A Sayed, Alaa H Lotfy, Mahmoud The Diagnostic Utility of Natural Killer Cell Subsets in Deep Vein Thrombosis |
title | The Diagnostic Utility of Natural Killer Cell Subsets in Deep Vein Thrombosis |
title_full | The Diagnostic Utility of Natural Killer Cell Subsets in Deep Vein Thrombosis |
title_fullStr | The Diagnostic Utility of Natural Killer Cell Subsets in Deep Vein Thrombosis |
title_full_unstemmed | The Diagnostic Utility of Natural Killer Cell Subsets in Deep Vein Thrombosis |
title_short | The Diagnostic Utility of Natural Killer Cell Subsets in Deep Vein Thrombosis |
title_sort | diagnostic utility of natural killer cell subsets in deep vein thrombosis |
topic | Original Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10680478/ https://www.ncbi.nlm.nih.gov/pubmed/38025520 http://dx.doi.org/10.2147/VHRM.S430466 |
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