Optical constraints on two-photon voltage imaging

Genetically encoded voltage indicators (GEVIs) are a valuable tool for studying neural circuits in vivo, but the relative merits of one-photon (1P) vs. two-photon (2P) voltage imaging are not well characterized. Here we compare the photophysical and imaging properties of commonly used GEVIs under 1P...

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Autores principales: Davis, Hunter C., Brooks, F. Phil, Wong-Campos, J. David, Cohen, Adam E.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Cold Spring Harbor Laboratory 2023
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10680948/
https://www.ncbi.nlm.nih.gov/pubmed/38014011
http://dx.doi.org/10.1101/2023.11.18.567441
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author Davis, Hunter C.
Brooks, F. Phil
Wong-Campos, J. David
Cohen, Adam E.
author_facet Davis, Hunter C.
Brooks, F. Phil
Wong-Campos, J. David
Cohen, Adam E.
author_sort Davis, Hunter C.
collection PubMed
description Genetically encoded voltage indicators (GEVIs) are a valuable tool for studying neural circuits in vivo, but the relative merits of one-photon (1P) vs. two-photon (2P) voltage imaging are not well characterized. Here we compare the photophysical and imaging properties of commonly used GEVIs under 1P and 2P excitation. 2P excitation requires ~10(4)-fold more illumination power per cell to produce comparable photon count rates to 1P excitation, driving a stringent tradeoff between shot noise and tissue photodamage.
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spelling pubmed-106809482023-11-27 Optical constraints on two-photon voltage imaging Davis, Hunter C. Brooks, F. Phil Wong-Campos, J. David Cohen, Adam E. bioRxiv Article Genetically encoded voltage indicators (GEVIs) are a valuable tool for studying neural circuits in vivo, but the relative merits of one-photon (1P) vs. two-photon (2P) voltage imaging are not well characterized. Here we compare the photophysical and imaging properties of commonly used GEVIs under 1P and 2P excitation. 2P excitation requires ~10(4)-fold more illumination power per cell to produce comparable photon count rates to 1P excitation, driving a stringent tradeoff between shot noise and tissue photodamage. Cold Spring Harbor Laboratory 2023-11-18 /pmc/articles/PMC10680948/ /pubmed/38014011 http://dx.doi.org/10.1101/2023.11.18.567441 Text en https://creativecommons.org/licenses/by-nc/4.0/This work is licensed under a Creative Commons Attribution-NonCommercial 4.0 International License (https://creativecommons.org/licenses/by-nc/4.0/) , which allows reusers to distribute, remix, adapt, and build upon the material in any medium or format for noncommercial purposes only, and only so long as attribution is given to the creator.
spellingShingle Article
Davis, Hunter C.
Brooks, F. Phil
Wong-Campos, J. David
Cohen, Adam E.
Optical constraints on two-photon voltage imaging
title Optical constraints on two-photon voltage imaging
title_full Optical constraints on two-photon voltage imaging
title_fullStr Optical constraints on two-photon voltage imaging
title_full_unstemmed Optical constraints on two-photon voltage imaging
title_short Optical constraints on two-photon voltage imaging
title_sort optical constraints on two-photon voltage imaging
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10680948/
https://www.ncbi.nlm.nih.gov/pubmed/38014011
http://dx.doi.org/10.1101/2023.11.18.567441
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