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Unveiling plant defense arsenal: metabolic strategies in Brassica oleracea during black rot disease
Alterations in plant metabolism play a key role in the complex plant–pathogen interactions. However, there is still a lack of knowledge about the connection between changes in primary and specialized metabolism and the plant defense against diseases that impact crops. Thus, we aim to study the metab...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Oxford University Press
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10681004/ https://www.ncbi.nlm.nih.gov/pubmed/38023479 http://dx.doi.org/10.1093/hr/uhad204 |
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author | Vega-Álvarez, Carmen Soengas, Pilar Roitsch, Thomas Abilleira, Rosaura Velasco, Pablo Francisco, Marta |
author_facet | Vega-Álvarez, Carmen Soengas, Pilar Roitsch, Thomas Abilleira, Rosaura Velasco, Pablo Francisco, Marta |
author_sort | Vega-Álvarez, Carmen |
collection | PubMed |
description | Alterations in plant metabolism play a key role in the complex plant–pathogen interactions. However, there is still a lack of knowledge about the connection between changes in primary and specialized metabolism and the plant defense against diseases that impact crops. Thus, we aim to study the metabolic reprograming in Brassica oleracea plants upon infection by Xanthomonas campestris pv. campestris (Xcc). To accomplish this, we utilized a combination of untargeted and targeted metabolomics, through UPLC-Q-TOF-MS/MS and (1)H-NMR, in two crop lines differing in resistance that were evaluated at two- and four-week intervals following inoculation (T1 and T2, respectively). Besides, to depict the physiological status of the plant during infection, enzymatic activities related to the carbohydrate pathway and oxidative stress were studied. Our results revealed different temporal dynamics in the responses of the susceptible vs. resistant crops lines. Resistant B. oleracea line suppresses carbohydrate metabolism contributing to limit nutrient supplies to the bacterium and prioritizes the induction of defensive compounds such as indolic glucosinolates, salicylic acid, phenylpropanoids and phytoalexins precursors at early infection stages. In contrast, the susceptible line invests in carbohydrate metabolism, including enzymatic activities related to the hexoses turnover, and activates defense signaling related to reactive oxygen species. Thus, each line triggers a different metabolic strategy that will affect how the plant overcomes the disease in terms of resistance and growth. This work provides first insights of a fine-tuned metabolic regulation during Xcc infection in B. oleracea that will contribute to develop new strategies for plant disease management. |
format | Online Article Text |
id | pubmed-10681004 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Oxford University Press |
record_format | MEDLINE/PubMed |
spelling | pubmed-106810042023-10-13 Unveiling plant defense arsenal: metabolic strategies in Brassica oleracea during black rot disease Vega-Álvarez, Carmen Soengas, Pilar Roitsch, Thomas Abilleira, Rosaura Velasco, Pablo Francisco, Marta Hortic Res Article Alterations in plant metabolism play a key role in the complex plant–pathogen interactions. However, there is still a lack of knowledge about the connection between changes in primary and specialized metabolism and the plant defense against diseases that impact crops. Thus, we aim to study the metabolic reprograming in Brassica oleracea plants upon infection by Xanthomonas campestris pv. campestris (Xcc). To accomplish this, we utilized a combination of untargeted and targeted metabolomics, through UPLC-Q-TOF-MS/MS and (1)H-NMR, in two crop lines differing in resistance that were evaluated at two- and four-week intervals following inoculation (T1 and T2, respectively). Besides, to depict the physiological status of the plant during infection, enzymatic activities related to the carbohydrate pathway and oxidative stress were studied. Our results revealed different temporal dynamics in the responses of the susceptible vs. resistant crops lines. Resistant B. oleracea line suppresses carbohydrate metabolism contributing to limit nutrient supplies to the bacterium and prioritizes the induction of defensive compounds such as indolic glucosinolates, salicylic acid, phenylpropanoids and phytoalexins precursors at early infection stages. In contrast, the susceptible line invests in carbohydrate metabolism, including enzymatic activities related to the hexoses turnover, and activates defense signaling related to reactive oxygen species. Thus, each line triggers a different metabolic strategy that will affect how the plant overcomes the disease in terms of resistance and growth. This work provides first insights of a fine-tuned metabolic regulation during Xcc infection in B. oleracea that will contribute to develop new strategies for plant disease management. Oxford University Press 2023-10-13 /pmc/articles/PMC10681004/ /pubmed/38023479 http://dx.doi.org/10.1093/hr/uhad204 Text en © The Author(s) 2023. Published by Oxford University Press on behalf of Nanjing Agricultural University. https://creativecommons.org/licenses/by/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Article Vega-Álvarez, Carmen Soengas, Pilar Roitsch, Thomas Abilleira, Rosaura Velasco, Pablo Francisco, Marta Unveiling plant defense arsenal: metabolic strategies in Brassica oleracea during black rot disease |
title | Unveiling plant defense arsenal: metabolic strategies in Brassica oleracea during black rot disease |
title_full | Unveiling plant defense arsenal: metabolic strategies in Brassica oleracea during black rot disease |
title_fullStr | Unveiling plant defense arsenal: metabolic strategies in Brassica oleracea during black rot disease |
title_full_unstemmed | Unveiling plant defense arsenal: metabolic strategies in Brassica oleracea during black rot disease |
title_short | Unveiling plant defense arsenal: metabolic strategies in Brassica oleracea during black rot disease |
title_sort | unveiling plant defense arsenal: metabolic strategies in brassica oleracea during black rot disease |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10681004/ https://www.ncbi.nlm.nih.gov/pubmed/38023479 http://dx.doi.org/10.1093/hr/uhad204 |
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