Cargando…
Sperm cryopreservation in Windsnyer boars; principles, technique, and updated outcomes
The domestic pig breeds are in danger of extinction whereas the erosion of their gene pool is a serious concern because they significantly contribute to the rich biodiversity. Overall aim of this study was to determine the protocol for preserving the semen of the Windsnyer boars for conservation. A...
Autores principales: | , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Colégio Brasileiro de Reprodução Animal
2023
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10681127/ https://www.ncbi.nlm.nih.gov/pubmed/38025997 http://dx.doi.org/10.1590/1984-3143-AR2022-0100 |
_version_ | 1785142448425533440 |
---|---|
author | Thema, Mamonene Angelinah Mphaphathi, Masindi Lottus Ledwaba, Mahlatsana Ramaesela Nedambale, Tshimangadzo Lucky |
author_facet | Thema, Mamonene Angelinah Mphaphathi, Masindi Lottus Ledwaba, Mahlatsana Ramaesela Nedambale, Tshimangadzo Lucky |
author_sort | Thema, Mamonene Angelinah |
collection | PubMed |
description | The domestic pig breeds are in danger of extinction whereas the erosion of their gene pool is a serious concern because they significantly contribute to the rich biodiversity. Overall aim of this study was to determine the protocol for preserving the semen of the Windsnyer boars for conservation. A total of 18 ejaculates (6 replications/boar) were collected from three Windsnyer boars of proven fertility with the use of hand-gloved approach method, twice per week. Boars semen were pooled and extended with Beltsville Thawing Solution [(BTS) IMV Technologies, France], held at 18°C for 3 hours and centrifuged. The sperm pellet was re-suspended with Fraction A (20% egg yolk + BTS) and cooled at 5°C for 1 hour. Following cooling, semen was divided and diluted into different cryoprotectants (ethylene glycol, glycerol, propanediol, ethylene glycol + glycerol + propanediol) at equal contribution to make the total concentrations [4, 8, 12 and 16% and the 0% (control; without cryoprotectant)] and loaded into 0.25 mL straws. Two cryopreservation methods (liquid nitrogen vapour and controlled rated) were used to cryopreserve the semen straws. Semen straws were thawed at different temperatures (5, 18, 37 and 40°C) and evaluated for sperm motility, viability, and morphology traits. Post-thawed sperm total motility (36.0±5.3) and live normal sperm (49.5±8.3) percentages were recorded to be higher in the treatment supplemented with 16% glycerol (P<0.05). The highest sperm total motility percentage was recorded at 40°C (26.8±3.2) thawing temperature for liquid nitrogen vapour treatment (P<0.05). In conclusion, 16% glycerol was found to be the suitable cryoprotectant concentration for semen cryopreserved with liquid nitrogen vapour method and thawed at 40°C. |
format | Online Article Text |
id | pubmed-10681127 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Colégio Brasileiro de Reprodução Animal |
record_format | MEDLINE/PubMed |
spelling | pubmed-106811272023-10-09 Sperm cryopreservation in Windsnyer boars; principles, technique, and updated outcomes Thema, Mamonene Angelinah Mphaphathi, Masindi Lottus Ledwaba, Mahlatsana Ramaesela Nedambale, Tshimangadzo Lucky Anim Reprod Short Communication The domestic pig breeds are in danger of extinction whereas the erosion of their gene pool is a serious concern because they significantly contribute to the rich biodiversity. Overall aim of this study was to determine the protocol for preserving the semen of the Windsnyer boars for conservation. A total of 18 ejaculates (6 replications/boar) were collected from three Windsnyer boars of proven fertility with the use of hand-gloved approach method, twice per week. Boars semen were pooled and extended with Beltsville Thawing Solution [(BTS) IMV Technologies, France], held at 18°C for 3 hours and centrifuged. The sperm pellet was re-suspended with Fraction A (20% egg yolk + BTS) and cooled at 5°C for 1 hour. Following cooling, semen was divided and diluted into different cryoprotectants (ethylene glycol, glycerol, propanediol, ethylene glycol + glycerol + propanediol) at equal contribution to make the total concentrations [4, 8, 12 and 16% and the 0% (control; without cryoprotectant)] and loaded into 0.25 mL straws. Two cryopreservation methods (liquid nitrogen vapour and controlled rated) were used to cryopreserve the semen straws. Semen straws were thawed at different temperatures (5, 18, 37 and 40°C) and evaluated for sperm motility, viability, and morphology traits. Post-thawed sperm total motility (36.0±5.3) and live normal sperm (49.5±8.3) percentages were recorded to be higher in the treatment supplemented with 16% glycerol (P<0.05). The highest sperm total motility percentage was recorded at 40°C (26.8±3.2) thawing temperature for liquid nitrogen vapour treatment (P<0.05). In conclusion, 16% glycerol was found to be the suitable cryoprotectant concentration for semen cryopreserved with liquid nitrogen vapour method and thawed at 40°C. Colégio Brasileiro de Reprodução Animal 2023-10-09 /pmc/articles/PMC10681127/ /pubmed/38025997 http://dx.doi.org/10.1590/1984-3143-AR2022-0100 Text en https://creativecommons.org/licenses/by/4.0/Copyright © The Author(s). This is an Open Access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Short Communication Thema, Mamonene Angelinah Mphaphathi, Masindi Lottus Ledwaba, Mahlatsana Ramaesela Nedambale, Tshimangadzo Lucky Sperm cryopreservation in Windsnyer boars; principles, technique, and updated outcomes |
title | Sperm cryopreservation in Windsnyer boars; principles, technique, and updated outcomes |
title_full | Sperm cryopreservation in Windsnyer boars; principles, technique, and updated outcomes |
title_fullStr | Sperm cryopreservation in Windsnyer boars; principles, technique, and updated outcomes |
title_full_unstemmed | Sperm cryopreservation in Windsnyer boars; principles, technique, and updated outcomes |
title_short | Sperm cryopreservation in Windsnyer boars; principles, technique, and updated outcomes |
title_sort | sperm cryopreservation in windsnyer boars; principles, technique, and updated outcomes |
topic | Short Communication |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10681127/ https://www.ncbi.nlm.nih.gov/pubmed/38025997 http://dx.doi.org/10.1590/1984-3143-AR2022-0100 |
work_keys_str_mv | AT themamamoneneangelinah spermcryopreservationinwindsnyerboarsprinciplestechniqueandupdatedoutcomes AT mphaphathimasindilottus spermcryopreservationinwindsnyerboarsprinciplestechniqueandupdatedoutcomes AT ledwabamahlatsanaramaesela spermcryopreservationinwindsnyerboarsprinciplestechniqueandupdatedoutcomes AT nedambaletshimangadzolucky spermcryopreservationinwindsnyerboarsprinciplestechniqueandupdatedoutcomes |