Cargando…

Synthesis and analyses of injectable fluoridated-bioactive glass hydrogel for dental root canal sealing

This study aimed to synthesize fluoride-doped bioactive glass (F-BG) based thermo-sensitive injectable hydrogel for endodontic applications. The structural and phase analyses were done with Fourier Transform Infrared spectroscopy and X-ray Diffraction, respectively. The setting time of prepared inje...

Descripción completa

Detalles Bibliográficos
Autores principales: Irshad, Nadia, Jahanzeb, Noureen, Alqasim, Alanood, Bousaleh, Raneem, Almehrij, Maha, Ghafoor, Sarah, Nawaz, Muhammad, Akhtar, Sultan, Tufail Shah, Asma, Khan, Abdul Samad
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10681180/
https://www.ncbi.nlm.nih.gov/pubmed/38011158
http://dx.doi.org/10.1371/journal.pone.0294446
_version_ 1785150763403575296
author Irshad, Nadia
Jahanzeb, Noureen
Alqasim, Alanood
Bousaleh, Raneem
Almehrij, Maha
Ghafoor, Sarah
Nawaz, Muhammad
Akhtar, Sultan
Tufail Shah, Asma
Khan, Abdul Samad
author_facet Irshad, Nadia
Jahanzeb, Noureen
Alqasim, Alanood
Bousaleh, Raneem
Almehrij, Maha
Ghafoor, Sarah
Nawaz, Muhammad
Akhtar, Sultan
Tufail Shah, Asma
Khan, Abdul Samad
author_sort Irshad, Nadia
collection PubMed
description This study aimed to synthesize fluoride-doped bioactive glass (F-BG) based thermo-sensitive injectable hydrogel for endodontic applications. The structural and phase analyses were done with Fourier Transform Infrared spectroscopy and X-ray Diffraction, respectively. The setting time of prepared injectable was investigated at 21°C (in the presence and absence of an ultrasonic scalar) and at 37°C. Flowability was tested according to ISO-6876:2012 specifications, whereas injectability was checked by extrusion method using 21-, 22-, and 23-gauge needles. The in vitro bio-adhesion and push-out bond strength were studied on days 7 and 90 and compared with the commercially available TotalFill®. The ion release profile was analyzed for up to 30 days with Inductively Coupled Plasma Optical Emission Spectroscopy. The fluoride release analysis was conducted periodically for up to 21 days in deionized water and artificial saliva using an ion-selective electrode. The final setting time at 21°C, 21°C+ultrasonic scalar, and 37°C were 38.66±3.21, 29.12±1.23, and 32±3.46 min, respectively. The flowability was 25±3.94 mm, and the injectability coefficient was ≥70.3 for 22, 21, and 57% in a 23-gauge needle. Fluoride release in deionized water was found to be significantly higher than in artificial saliva and increased with time. A significant difference in bond strength was found between days 7 and 90, where the strength was increased, and a new apatite layer was formed on the tooth surface. A rapid release of calcium, phosphate, and silicon ions was seen initially, whereby the continuous release of these ions was observed for up to 30 days. The prepared F-BG injectable hydrogel has shown promising results and has the potential to be used as an endodontic sealer.
format Online
Article
Text
id pubmed-10681180
institution National Center for Biotechnology Information
language English
publishDate 2023
publisher Public Library of Science
record_format MEDLINE/PubMed
spelling pubmed-106811802023-11-27 Synthesis and analyses of injectable fluoridated-bioactive glass hydrogel for dental root canal sealing Irshad, Nadia Jahanzeb, Noureen Alqasim, Alanood Bousaleh, Raneem Almehrij, Maha Ghafoor, Sarah Nawaz, Muhammad Akhtar, Sultan Tufail Shah, Asma Khan, Abdul Samad PLoS One Research Article This study aimed to synthesize fluoride-doped bioactive glass (F-BG) based thermo-sensitive injectable hydrogel for endodontic applications. The structural and phase analyses were done with Fourier Transform Infrared spectroscopy and X-ray Diffraction, respectively. The setting time of prepared injectable was investigated at 21°C (in the presence and absence of an ultrasonic scalar) and at 37°C. Flowability was tested according to ISO-6876:2012 specifications, whereas injectability was checked by extrusion method using 21-, 22-, and 23-gauge needles. The in vitro bio-adhesion and push-out bond strength were studied on days 7 and 90 and compared with the commercially available TotalFill®. The ion release profile was analyzed for up to 30 days with Inductively Coupled Plasma Optical Emission Spectroscopy. The fluoride release analysis was conducted periodically for up to 21 days in deionized water and artificial saliva using an ion-selective electrode. The final setting time at 21°C, 21°C+ultrasonic scalar, and 37°C were 38.66±3.21, 29.12±1.23, and 32±3.46 min, respectively. The flowability was 25±3.94 mm, and the injectability coefficient was ≥70.3 for 22, 21, and 57% in a 23-gauge needle. Fluoride release in deionized water was found to be significantly higher than in artificial saliva and increased with time. A significant difference in bond strength was found between days 7 and 90, where the strength was increased, and a new apatite layer was formed on the tooth surface. A rapid release of calcium, phosphate, and silicon ions was seen initially, whereby the continuous release of these ions was observed for up to 30 days. The prepared F-BG injectable hydrogel has shown promising results and has the potential to be used as an endodontic sealer. Public Library of Science 2023-11-27 /pmc/articles/PMC10681180/ /pubmed/38011158 http://dx.doi.org/10.1371/journal.pone.0294446 Text en © 2023 Irshad et al https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Irshad, Nadia
Jahanzeb, Noureen
Alqasim, Alanood
Bousaleh, Raneem
Almehrij, Maha
Ghafoor, Sarah
Nawaz, Muhammad
Akhtar, Sultan
Tufail Shah, Asma
Khan, Abdul Samad
Synthesis and analyses of injectable fluoridated-bioactive glass hydrogel for dental root canal sealing
title Synthesis and analyses of injectable fluoridated-bioactive glass hydrogel for dental root canal sealing
title_full Synthesis and analyses of injectable fluoridated-bioactive glass hydrogel for dental root canal sealing
title_fullStr Synthesis and analyses of injectable fluoridated-bioactive glass hydrogel for dental root canal sealing
title_full_unstemmed Synthesis and analyses of injectable fluoridated-bioactive glass hydrogel for dental root canal sealing
title_short Synthesis and analyses of injectable fluoridated-bioactive glass hydrogel for dental root canal sealing
title_sort synthesis and analyses of injectable fluoridated-bioactive glass hydrogel for dental root canal sealing
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10681180/
https://www.ncbi.nlm.nih.gov/pubmed/38011158
http://dx.doi.org/10.1371/journal.pone.0294446
work_keys_str_mv AT irshadnadia synthesisandanalysesofinjectablefluoridatedbioactiveglasshydrogelfordentalrootcanalsealing
AT jahanzebnoureen synthesisandanalysesofinjectablefluoridatedbioactiveglasshydrogelfordentalrootcanalsealing
AT alqasimalanood synthesisandanalysesofinjectablefluoridatedbioactiveglasshydrogelfordentalrootcanalsealing
AT bousalehraneem synthesisandanalysesofinjectablefluoridatedbioactiveglasshydrogelfordentalrootcanalsealing
AT almehrijmaha synthesisandanalysesofinjectablefluoridatedbioactiveglasshydrogelfordentalrootcanalsealing
AT ghafoorsarah synthesisandanalysesofinjectablefluoridatedbioactiveglasshydrogelfordentalrootcanalsealing
AT nawazmuhammad synthesisandanalysesofinjectablefluoridatedbioactiveglasshydrogelfordentalrootcanalsealing
AT akhtarsultan synthesisandanalysesofinjectablefluoridatedbioactiveglasshydrogelfordentalrootcanalsealing
AT tufailshahasma synthesisandanalysesofinjectablefluoridatedbioactiveglasshydrogelfordentalrootcanalsealing
AT khanabdulsamad synthesisandanalysesofinjectablefluoridatedbioactiveglasshydrogelfordentalrootcanalsealing