Cargando…

Meta-analysis of diagnostic accuracy of nucleic acid amplification tests for abdominal tuberculosis

BACKGROUND: Abdominal tuberculosis (TB) is a severe extrapulmonary TB, which can lead to serious complications. Early diagnosis and treatment are very important for the prognosis and the diagnosis of abdominal TB is still difficult. METHODS: We searched PubMed, the Cochrane Library, Embase, China Na...

Descripción completa

Detalles Bibliográficos
Autores principales: Shen, Yanqin, Fang, Likui, Ye, Bo, Yu, Guocan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10681219/
https://www.ncbi.nlm.nih.gov/pubmed/38011098
http://dx.doi.org/10.1371/journal.pone.0289336
_version_ 1785150772429717504
author Shen, Yanqin
Fang, Likui
Ye, Bo
Yu, Guocan
author_facet Shen, Yanqin
Fang, Likui
Ye, Bo
Yu, Guocan
author_sort Shen, Yanqin
collection PubMed
description BACKGROUND: Abdominal tuberculosis (TB) is a severe extrapulmonary TB, which can lead to serious complications. Early diagnosis and treatment are very important for the prognosis and the diagnosis of abdominal TB is still difficult. METHODS: We searched PubMed, the Cochrane Library, Embase, China National Knowledge Infrastructure, and the Wanfang database for studies evaluating the diagnostic accuracy of NAATs for abdominal TB until August 2020. Any types of study design with full text were sought and included. The risk of bias was assessed using the Quality Assessment of Diagnostic Accuracy Studies tool. Subgroup analysis, meta-regression analysis and sensitivity analysis were used to explore the sources of heterogeneity. Stata version 15.0 with the midas command packages was used to carry out meta-analyses. RESULTS: We included a total of 78 independent studies from 53 articles; 64 with CRS as the reference standard, and 14 with culture as the reference standard. The pooled sensitivity, specificity, and the areas under summary receiver operating characteristic (SROC) curves (AUC) were 58% (51%–64%; I(2) = 87%), 99% (97%–99%; I(2) = 81%), and 0.92 (0.89–0.94) compared with CRS, respectively. The pooled sensitivity, specificity, and the AUC values of the SROC were 80% (66%–90%; I(2) = 56%), 96% (92%–98%; I(2) = 84%), and 0.97 (0.95–0.98) compared with culture, respectively. The heterogeneity of sensitivity and specificity was significant. CONCLUSIONS: NAATs had excellent efficacy in the diagnosis of abdominal TB regardless of the reference standard and regardless of the subtype of abdominal TB. Multiplex PCR with multiple target genes may improve diagnostic sensitivity, and stool specimens may also be used for the diagnosis of abdominal TB in addition to tissue and ascites.
format Online
Article
Text
id pubmed-10681219
institution National Center for Biotechnology Information
language English
publishDate 2023
publisher Public Library of Science
record_format MEDLINE/PubMed
spelling pubmed-106812192023-11-27 Meta-analysis of diagnostic accuracy of nucleic acid amplification tests for abdominal tuberculosis Shen, Yanqin Fang, Likui Ye, Bo Yu, Guocan PLoS One Research Article BACKGROUND: Abdominal tuberculosis (TB) is a severe extrapulmonary TB, which can lead to serious complications. Early diagnosis and treatment are very important for the prognosis and the diagnosis of abdominal TB is still difficult. METHODS: We searched PubMed, the Cochrane Library, Embase, China National Knowledge Infrastructure, and the Wanfang database for studies evaluating the diagnostic accuracy of NAATs for abdominal TB until August 2020. Any types of study design with full text were sought and included. The risk of bias was assessed using the Quality Assessment of Diagnostic Accuracy Studies tool. Subgroup analysis, meta-regression analysis and sensitivity analysis were used to explore the sources of heterogeneity. Stata version 15.0 with the midas command packages was used to carry out meta-analyses. RESULTS: We included a total of 78 independent studies from 53 articles; 64 with CRS as the reference standard, and 14 with culture as the reference standard. The pooled sensitivity, specificity, and the areas under summary receiver operating characteristic (SROC) curves (AUC) were 58% (51%–64%; I(2) = 87%), 99% (97%–99%; I(2) = 81%), and 0.92 (0.89–0.94) compared with CRS, respectively. The pooled sensitivity, specificity, and the AUC values of the SROC were 80% (66%–90%; I(2) = 56%), 96% (92%–98%; I(2) = 84%), and 0.97 (0.95–0.98) compared with culture, respectively. The heterogeneity of sensitivity and specificity was significant. CONCLUSIONS: NAATs had excellent efficacy in the diagnosis of abdominal TB regardless of the reference standard and regardless of the subtype of abdominal TB. Multiplex PCR with multiple target genes may improve diagnostic sensitivity, and stool specimens may also be used for the diagnosis of abdominal TB in addition to tissue and ascites. Public Library of Science 2023-11-27 /pmc/articles/PMC10681219/ /pubmed/38011098 http://dx.doi.org/10.1371/journal.pone.0289336 Text en © 2023 Shen et al https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Research Article
Shen, Yanqin
Fang, Likui
Ye, Bo
Yu, Guocan
Meta-analysis of diagnostic accuracy of nucleic acid amplification tests for abdominal tuberculosis
title Meta-analysis of diagnostic accuracy of nucleic acid amplification tests for abdominal tuberculosis
title_full Meta-analysis of diagnostic accuracy of nucleic acid amplification tests for abdominal tuberculosis
title_fullStr Meta-analysis of diagnostic accuracy of nucleic acid amplification tests for abdominal tuberculosis
title_full_unstemmed Meta-analysis of diagnostic accuracy of nucleic acid amplification tests for abdominal tuberculosis
title_short Meta-analysis of diagnostic accuracy of nucleic acid amplification tests for abdominal tuberculosis
title_sort meta-analysis of diagnostic accuracy of nucleic acid amplification tests for abdominal tuberculosis
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10681219/
https://www.ncbi.nlm.nih.gov/pubmed/38011098
http://dx.doi.org/10.1371/journal.pone.0289336
work_keys_str_mv AT shenyanqin metaanalysisofdiagnosticaccuracyofnucleicacidamplificationtestsforabdominaltuberculosis
AT fanglikui metaanalysisofdiagnosticaccuracyofnucleicacidamplificationtestsforabdominaltuberculosis
AT yebo metaanalysisofdiagnosticaccuracyofnucleicacidamplificationtestsforabdominaltuberculosis
AT yuguocan metaanalysisofdiagnosticaccuracyofnucleicacidamplificationtestsforabdominaltuberculosis