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Differential expression of mechanotransduction complex genes in auditory/vestibular hair cells in zebrafish

Ciliated sensory cells such as photo- and olfactory receptors employ multiple types of opsins or hundreds of unique olfactory G-protein coupled receptors to respond to various wavelengths of light or odorants. With respect to hearing and balance, the mechanotransduction machinery involves fewer vari...

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Autores principales: Smith, Eliot T., Sun, Peng, Yu, Shengyang Kevin, Raible, David W., Nicolson, Teresa
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10682102/
https://www.ncbi.nlm.nih.gov/pubmed/38035267
http://dx.doi.org/10.3389/fnmol.2023.1274822
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author Smith, Eliot T.
Sun, Peng
Yu, Shengyang Kevin
Raible, David W.
Nicolson, Teresa
author_facet Smith, Eliot T.
Sun, Peng
Yu, Shengyang Kevin
Raible, David W.
Nicolson, Teresa
author_sort Smith, Eliot T.
collection PubMed
description Ciliated sensory cells such as photo- and olfactory receptors employ multiple types of opsins or hundreds of unique olfactory G-protein coupled receptors to respond to various wavelengths of light or odorants. With respect to hearing and balance, the mechanotransduction machinery involves fewer variants; however, emerging evidence suggests that specialization occurs at the molecular level. To address how the mechanotransduction complex varies in the inner ear, we characterized the expression of paralogous genes that encode components required for mechanotransduction in zebrafish hair cells using RNA-FISH and bioinformatic analysis. Our data indicate striking zonal differences in the expression of two components of the mechanotransduction complex which are known to physically interact, the transmembrane channel-like 1 and 2 (tmc1/2) family members and the calcium and integrin binding 2 and 3 (cib2/3) paralogues. tmc1, tmc2b, and cib3 are largely expressed in peripheral or extrastriolar hair cells, whereas tmc2a and cib2 are enriched in central or striolar hair cells. In addition, a gene implicated in deaf-blindness, ush1c, is highly enriched in a subset of extrastriolar hair cells. These results indicate that specific combinations of these components may optimize responses to mechanical stimuli in subtypes of sensory receptors within the inner ear.
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spelling pubmed-106821022023-11-30 Differential expression of mechanotransduction complex genes in auditory/vestibular hair cells in zebrafish Smith, Eliot T. Sun, Peng Yu, Shengyang Kevin Raible, David W. Nicolson, Teresa Front Mol Neurosci Molecular Neuroscience Ciliated sensory cells such as photo- and olfactory receptors employ multiple types of opsins or hundreds of unique olfactory G-protein coupled receptors to respond to various wavelengths of light or odorants. With respect to hearing and balance, the mechanotransduction machinery involves fewer variants; however, emerging evidence suggests that specialization occurs at the molecular level. To address how the mechanotransduction complex varies in the inner ear, we characterized the expression of paralogous genes that encode components required for mechanotransduction in zebrafish hair cells using RNA-FISH and bioinformatic analysis. Our data indicate striking zonal differences in the expression of two components of the mechanotransduction complex which are known to physically interact, the transmembrane channel-like 1 and 2 (tmc1/2) family members and the calcium and integrin binding 2 and 3 (cib2/3) paralogues. tmc1, tmc2b, and cib3 are largely expressed in peripheral or extrastriolar hair cells, whereas tmc2a and cib2 are enriched in central or striolar hair cells. In addition, a gene implicated in deaf-blindness, ush1c, is highly enriched in a subset of extrastriolar hair cells. These results indicate that specific combinations of these components may optimize responses to mechanical stimuli in subtypes of sensory receptors within the inner ear. Frontiers Media S.A. 2023-11-14 /pmc/articles/PMC10682102/ /pubmed/38035267 http://dx.doi.org/10.3389/fnmol.2023.1274822 Text en Copyright © 2023 Smith, Sun, Yu, Raible and Nicolson. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Molecular Neuroscience
Smith, Eliot T.
Sun, Peng
Yu, Shengyang Kevin
Raible, David W.
Nicolson, Teresa
Differential expression of mechanotransduction complex genes in auditory/vestibular hair cells in zebrafish
title Differential expression of mechanotransduction complex genes in auditory/vestibular hair cells in zebrafish
title_full Differential expression of mechanotransduction complex genes in auditory/vestibular hair cells in zebrafish
title_fullStr Differential expression of mechanotransduction complex genes in auditory/vestibular hair cells in zebrafish
title_full_unstemmed Differential expression of mechanotransduction complex genes in auditory/vestibular hair cells in zebrafish
title_short Differential expression of mechanotransduction complex genes in auditory/vestibular hair cells in zebrafish
title_sort differential expression of mechanotransduction complex genes in auditory/vestibular hair cells in zebrafish
topic Molecular Neuroscience
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10682102/
https://www.ncbi.nlm.nih.gov/pubmed/38035267
http://dx.doi.org/10.3389/fnmol.2023.1274822
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