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Detection of mecA positive staphylococcal species in a wastewater treatment plant in South Africa

We investigated the prevalence of antibiotic resistant staphylococci and detection of resistant, virulence, and Spa genes in a South African wastewater treatment plant. Species identified were Staphylococcus aureus, S. lentus, S. arlettae, S. cohnii, S. haemolyticus, S. nepalensis, S. sciuri (now Ma...

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Autores principales: Oladipo, Adegboyega Oyedele, Oladipo, Oluwatosin Gbemisola, Bezuidenhout, Carlos Cornelius
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer Berlin Heidelberg 2023
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Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10682275/
https://www.ncbi.nlm.nih.gov/pubmed/37864690
http://dx.doi.org/10.1007/s11356-023-30319-9
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author Oladipo, Adegboyega Oyedele
Oladipo, Oluwatosin Gbemisola
Bezuidenhout, Carlos Cornelius
author_facet Oladipo, Adegboyega Oyedele
Oladipo, Oluwatosin Gbemisola
Bezuidenhout, Carlos Cornelius
author_sort Oladipo, Adegboyega Oyedele
collection PubMed
description We investigated the prevalence of antibiotic resistant staphylococci and detection of resistant, virulence, and Spa genes in a South African wastewater treatment plant. Species identified were Staphylococcus aureus, S. lentus, S. arlettae, S. cohnii, S. haemolyticus, S. nepalensis, S. sciuri (now Mammaliicoccus sciuri), and S. xylosus. Isolates showed high resistance to methicillin (91%), ampicillin (89%), ciprofloxacin (86%), amoxycillin (80%), ceftazidime (74%), and cloxacillin (71%). Multiple antibiotic resistance (MAR) index for the isolates exceeded 0.2 (0.50–0.70). Among the isolates, 77% were mecA-positive. All S. aureus strains were positive for nuc and 7 Spa gene types. The present study highlights possibility of treated wastewaters being potential reservoir for antibiotic-resistant staphylococci. This is a cause for concern as wastewater effluents are decanted into environmental waters and these are, in many cases, used for various purposes including recreation (full contact), religious (full body submersion), and drinking water for some rural communities and water for livestock.
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spelling pubmed-106822752023-11-30 Detection of mecA positive staphylococcal species in a wastewater treatment plant in South Africa Oladipo, Adegboyega Oyedele Oladipo, Oluwatosin Gbemisola Bezuidenhout, Carlos Cornelius Environ Sci Pollut Res Int Research Article We investigated the prevalence of antibiotic resistant staphylococci and detection of resistant, virulence, and Spa genes in a South African wastewater treatment plant. Species identified were Staphylococcus aureus, S. lentus, S. arlettae, S. cohnii, S. haemolyticus, S. nepalensis, S. sciuri (now Mammaliicoccus sciuri), and S. xylosus. Isolates showed high resistance to methicillin (91%), ampicillin (89%), ciprofloxacin (86%), amoxycillin (80%), ceftazidime (74%), and cloxacillin (71%). Multiple antibiotic resistance (MAR) index for the isolates exceeded 0.2 (0.50–0.70). Among the isolates, 77% were mecA-positive. All S. aureus strains were positive for nuc and 7 Spa gene types. The present study highlights possibility of treated wastewaters being potential reservoir for antibiotic-resistant staphylococci. This is a cause for concern as wastewater effluents are decanted into environmental waters and these are, in many cases, used for various purposes including recreation (full contact), religious (full body submersion), and drinking water for some rural communities and water for livestock. Springer Berlin Heidelberg 2023-10-21 2023 /pmc/articles/PMC10682275/ /pubmed/37864690 http://dx.doi.org/10.1007/s11356-023-30319-9 Text en © The Author(s) 2023 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Research Article
Oladipo, Adegboyega Oyedele
Oladipo, Oluwatosin Gbemisola
Bezuidenhout, Carlos Cornelius
Detection of mecA positive staphylococcal species in a wastewater treatment plant in South Africa
title Detection of mecA positive staphylococcal species in a wastewater treatment plant in South Africa
title_full Detection of mecA positive staphylococcal species in a wastewater treatment plant in South Africa
title_fullStr Detection of mecA positive staphylococcal species in a wastewater treatment plant in South Africa
title_full_unstemmed Detection of mecA positive staphylococcal species in a wastewater treatment plant in South Africa
title_short Detection of mecA positive staphylococcal species in a wastewater treatment plant in South Africa
title_sort detection of meca positive staphylococcal species in a wastewater treatment plant in south africa
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10682275/
https://www.ncbi.nlm.nih.gov/pubmed/37864690
http://dx.doi.org/10.1007/s11356-023-30319-9
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