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Plasma IL-33 levels and immune activation in HIV-TB coinfection: a cross-sectional study in Yaoundé, Cameroon
INTRODUCTION: HIV-1 and Mtb are characterized by immune activation and unbalances production of cytokines, but the expression of IL33 in HIV/TB coinfection remain understudied. This study aimed to evaluate the level of IL-33 in plasma of HIV and M. tuberculosis (HIV/TB) coinfected patients compared...
Autores principales: | , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The African Field Epidemiology Network
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10683167/ https://www.ncbi.nlm.nih.gov/pubmed/38035159 http://dx.doi.org/10.11604/pamj.2023.46.13.41152 |
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author | Essomba, René Ghislain Mbe, Rostand Munkam Ngogang, Marie Paule Ekono, Claire Bitchong Bitoungui, Valentina Josiane Ngo Seni, Nassif Nguwoh, Philippe Salomon Ateba, Pulchérie Thérèse Kamdem, Severin Donald Nono, Justin Komguep Ambomo, Myriam Sylvie Assoumou, Marie Claire Okomo Mbopi-Kéou, François Xavier |
author_facet | Essomba, René Ghislain Mbe, Rostand Munkam Ngogang, Marie Paule Ekono, Claire Bitchong Bitoungui, Valentina Josiane Ngo Seni, Nassif Nguwoh, Philippe Salomon Ateba, Pulchérie Thérèse Kamdem, Severin Donald Nono, Justin Komguep Ambomo, Myriam Sylvie Assoumou, Marie Claire Okomo Mbopi-Kéou, François Xavier |
author_sort | Essomba, René Ghislain |
collection | PubMed |
description | INTRODUCTION: HIV-1 and Mtb are characterized by immune activation and unbalances production of cytokines, but the expression of IL33 in HIV/TB coinfection remain understudied. This study aimed to evaluate the level of IL-33 in plasma of HIV and M. tuberculosis (HIV/TB) coinfected patients compared to patients with respective mono infections in Yaoundé. METHODS: a cross-sectional study was conducted among patients attending the pneumology service and HIV treatment center of the Yaoundé Jamot Hospital. Plasma samples of 157 HIV/TB coinfected patients (n =26, 50% males and 50% females, mean age 39), HIV-1 monoinfected patients (n = 41, 41% males and 59% females, mean age 35), TB monoinfected patients (n = 48, 56% males and 44% females, mean age 37) and healthy controls (n = 42, 29% males and 71% females, mean age 32) were examined by enzyme-linked immunoassay (ELISA) to detect the levels of IL-33 cytokine. RESULTS: plasma level of IL-33 were higher in HIV/TB coinfected (33.1±30.9 pg/ml) and TB monoinfected individuals (15.1±2.9 pg/ml) compared to healthy controls (14.0±3.4 pg/ml) and could not be detected in most of the HIV-1 monoinfected individuals (12.6±8.7 pg/ml). Interestingly, the increased plasma level of IL-33 in HIV/TB coinfected patients showed a statistically significant difference between healthy controls (33.1±30.9 pg/ml vs 14.0±3.4 pg/ml, P<0.0001) and HIV-1 monoinfected patients (33.1±30.9 pg/ml vs 12.6±8.7 pg/ml, P=0.0002). We further found that IL-33 was higher in patients with high viral load group (40.6±59.7 pg/ml vs 12.6±1.8 pg/ml), P= 0.47) whereas patients under highly active antiretroviral therapy (HAART) showed decreased level of IL-33 concentration as the number of years under ART increased. Our data showed a positive association between plasma IL-33 and viral load in the context of HIV/TB coinfection in our study population with a positive Pearson coefficient of r=0.21. CONCLUSION: this study indicates that plasma level of IL-33 differs among HIV/TB coinfected patients and respective monoinfections patients. The increased level of plasma IL-33 reveals that IL-33 measurement in HIV-1 monoinfected patients may represent an early predictor of development of tuberculosis. |
format | Online Article Text |
id | pubmed-10683167 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | The African Field Epidemiology Network |
record_format | MEDLINE/PubMed |
spelling | pubmed-106831672023-11-30 Plasma IL-33 levels and immune activation in HIV-TB coinfection: a cross-sectional study in Yaoundé, Cameroon Essomba, René Ghislain Mbe, Rostand Munkam Ngogang, Marie Paule Ekono, Claire Bitchong Bitoungui, Valentina Josiane Ngo Seni, Nassif Nguwoh, Philippe Salomon Ateba, Pulchérie Thérèse Kamdem, Severin Donald Nono, Justin Komguep Ambomo, Myriam Sylvie Assoumou, Marie Claire Okomo Mbopi-Kéou, François Xavier Pan Afr Med J Research INTRODUCTION: HIV-1 and Mtb are characterized by immune activation and unbalances production of cytokines, but the expression of IL33 in HIV/TB coinfection remain understudied. This study aimed to evaluate the level of IL-33 in plasma of HIV and M. tuberculosis (HIV/TB) coinfected patients compared to patients with respective mono infections in Yaoundé. METHODS: a cross-sectional study was conducted among patients attending the pneumology service and HIV treatment center of the Yaoundé Jamot Hospital. Plasma samples of 157 HIV/TB coinfected patients (n =26, 50% males and 50% females, mean age 39), HIV-1 monoinfected patients (n = 41, 41% males and 59% females, mean age 35), TB monoinfected patients (n = 48, 56% males and 44% females, mean age 37) and healthy controls (n = 42, 29% males and 71% females, mean age 32) were examined by enzyme-linked immunoassay (ELISA) to detect the levels of IL-33 cytokine. RESULTS: plasma level of IL-33 were higher in HIV/TB coinfected (33.1±30.9 pg/ml) and TB monoinfected individuals (15.1±2.9 pg/ml) compared to healthy controls (14.0±3.4 pg/ml) and could not be detected in most of the HIV-1 monoinfected individuals (12.6±8.7 pg/ml). Interestingly, the increased plasma level of IL-33 in HIV/TB coinfected patients showed a statistically significant difference between healthy controls (33.1±30.9 pg/ml vs 14.0±3.4 pg/ml, P<0.0001) and HIV-1 monoinfected patients (33.1±30.9 pg/ml vs 12.6±8.7 pg/ml, P=0.0002). We further found that IL-33 was higher in patients with high viral load group (40.6±59.7 pg/ml vs 12.6±1.8 pg/ml), P= 0.47) whereas patients under highly active antiretroviral therapy (HAART) showed decreased level of IL-33 concentration as the number of years under ART increased. Our data showed a positive association between plasma IL-33 and viral load in the context of HIV/TB coinfection in our study population with a positive Pearson coefficient of r=0.21. CONCLUSION: this study indicates that plasma level of IL-33 differs among HIV/TB coinfected patients and respective monoinfections patients. The increased level of plasma IL-33 reveals that IL-33 measurement in HIV-1 monoinfected patients may represent an early predictor of development of tuberculosis. The African Field Epidemiology Network 2023-09-11 /pmc/articles/PMC10683167/ /pubmed/38035159 http://dx.doi.org/10.11604/pamj.2023.46.13.41152 Text en Copyright: René Ghislain Essomba et al. https://creativecommons.org/licenses/by/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution International 4.0 License (https://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. |
spellingShingle | Research Essomba, René Ghislain Mbe, Rostand Munkam Ngogang, Marie Paule Ekono, Claire Bitchong Bitoungui, Valentina Josiane Ngo Seni, Nassif Nguwoh, Philippe Salomon Ateba, Pulchérie Thérèse Kamdem, Severin Donald Nono, Justin Komguep Ambomo, Myriam Sylvie Assoumou, Marie Claire Okomo Mbopi-Kéou, François Xavier Plasma IL-33 levels and immune activation in HIV-TB coinfection: a cross-sectional study in Yaoundé, Cameroon |
title | Plasma IL-33 levels and immune activation in HIV-TB coinfection: a cross-sectional study in Yaoundé, Cameroon |
title_full | Plasma IL-33 levels and immune activation in HIV-TB coinfection: a cross-sectional study in Yaoundé, Cameroon |
title_fullStr | Plasma IL-33 levels and immune activation in HIV-TB coinfection: a cross-sectional study in Yaoundé, Cameroon |
title_full_unstemmed | Plasma IL-33 levels and immune activation in HIV-TB coinfection: a cross-sectional study in Yaoundé, Cameroon |
title_short | Plasma IL-33 levels and immune activation in HIV-TB coinfection: a cross-sectional study in Yaoundé, Cameroon |
title_sort | plasma il-33 levels and immune activation in hiv-tb coinfection: a cross-sectional study in yaoundé, cameroon |
topic | Research |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10683167/ https://www.ncbi.nlm.nih.gov/pubmed/38035159 http://dx.doi.org/10.11604/pamj.2023.46.13.41152 |
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