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Microbial Upcycling of Waste PET to Adipic Acid
[Image: see text] Microorganisms can be genetically engineered to transform abundant waste feedstocks into value-added small molecules that would otherwise be manufactured from diminishing fossil resources. Herein, we report the first one-pot bio-upcycling of PET plastic waste into the prolific plat...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
American Chemical Society
2023
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10683474/ https://www.ncbi.nlm.nih.gov/pubmed/38033806 http://dx.doi.org/10.1021/acscentsci.3c00414 |
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author | Valenzuela-Ortega, Marcos Suitor, Jack T. White, Mirren F. M. Hinchcliffe, Trevor Wallace, Stephen |
author_facet | Valenzuela-Ortega, Marcos Suitor, Jack T. White, Mirren F. M. Hinchcliffe, Trevor Wallace, Stephen |
author_sort | Valenzuela-Ortega, Marcos |
collection | PubMed |
description | [Image: see text] Microorganisms can be genetically engineered to transform abundant waste feedstocks into value-added small molecules that would otherwise be manufactured from diminishing fossil resources. Herein, we report the first one-pot bio-upcycling of PET plastic waste into the prolific platform petrochemical and nylon precursor adipic acid in the bacterium Escherichia coli. Optimizing heterologous gene expression and enzyme activity enabled increased flux through the de novo pathway, and immobilization of whole cells in alginate hydrogels increased the stability of the rate-limiting enoate reductase BcER. The pathway enzymes were also interfaced with hydrogen gas generated by engineered E. coli DD-2 in combination with a biocompatible Pd catalyst to enable adipic acid synthesis from metabolic cis,cis-muconic acid. Together, these optimizations resulted in a one-pot conversion to adipic acid from terephthalic acid, including terephthalate samples isolated from industrial PET waste and a post-consumer plastic bottle. |
format | Online Article Text |
id | pubmed-10683474 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | American Chemical Society |
record_format | MEDLINE/PubMed |
spelling | pubmed-106834742023-11-30 Microbial Upcycling of Waste PET to Adipic Acid Valenzuela-Ortega, Marcos Suitor, Jack T. White, Mirren F. M. Hinchcliffe, Trevor Wallace, Stephen ACS Cent Sci [Image: see text] Microorganisms can be genetically engineered to transform abundant waste feedstocks into value-added small molecules that would otherwise be manufactured from diminishing fossil resources. Herein, we report the first one-pot bio-upcycling of PET plastic waste into the prolific platform petrochemical and nylon precursor adipic acid in the bacterium Escherichia coli. Optimizing heterologous gene expression and enzyme activity enabled increased flux through the de novo pathway, and immobilization of whole cells in alginate hydrogels increased the stability of the rate-limiting enoate reductase BcER. The pathway enzymes were also interfaced with hydrogen gas generated by engineered E. coli DD-2 in combination with a biocompatible Pd catalyst to enable adipic acid synthesis from metabolic cis,cis-muconic acid. Together, these optimizations resulted in a one-pot conversion to adipic acid from terephthalic acid, including terephthalate samples isolated from industrial PET waste and a post-consumer plastic bottle. American Chemical Society 2023-11-01 /pmc/articles/PMC10683474/ /pubmed/38033806 http://dx.doi.org/10.1021/acscentsci.3c00414 Text en © 2023 The Authors. Published by American Chemical Society https://creativecommons.org/licenses/by/4.0/Permits the broadest form of re-use including for commercial purposes, provided that author attribution and integrity are maintained (https://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Valenzuela-Ortega, Marcos Suitor, Jack T. White, Mirren F. M. Hinchcliffe, Trevor Wallace, Stephen Microbial Upcycling of Waste PET to Adipic Acid |
title | Microbial Upcycling
of Waste PET to Adipic Acid |
title_full | Microbial Upcycling
of Waste PET to Adipic Acid |
title_fullStr | Microbial Upcycling
of Waste PET to Adipic Acid |
title_full_unstemmed | Microbial Upcycling
of Waste PET to Adipic Acid |
title_short | Microbial Upcycling
of Waste PET to Adipic Acid |
title_sort | microbial upcycling
of waste pet to adipic acid |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10683474/ https://www.ncbi.nlm.nih.gov/pubmed/38033806 http://dx.doi.org/10.1021/acscentsci.3c00414 |
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