Bispecific Antibody Detection Using Antigen-Conjugated Synthetic Nucleic Acid Strands

[Image: see text] We report here the development of two different sensing strategies based on the use of antigen-conjugated nucleic acid strands for the detection of a bispecific antibody against the tumor-related proteins Mucin1 and epidermal growth factor receptor. Both approaches work well in ser...

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Autores principales: Mariottini, Davide, Bracaglia, Sara, Barbero, Luca, Fuchs, Sebastian W., Saal, Christoph, Moniot, Sebastien, Knuehl, Christine, Baranda, Lorena, Ranallo, Simona, Ricci, Francesco
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Chemical Society 2023
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10683503/
https://www.ncbi.nlm.nih.gov/pubmed/37856082
http://dx.doi.org/10.1021/acssensors.3c01717
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author Mariottini, Davide
Bracaglia, Sara
Barbero, Luca
Fuchs, Sebastian W.
Saal, Christoph
Moniot, Sebastien
Knuehl, Christine
Baranda, Lorena
Ranallo, Simona
Ricci, Francesco
author_facet Mariottini, Davide
Bracaglia, Sara
Barbero, Luca
Fuchs, Sebastian W.
Saal, Christoph
Moniot, Sebastien
Knuehl, Christine
Baranda, Lorena
Ranallo, Simona
Ricci, Francesco
author_sort Mariottini, Davide
collection PubMed
description [Image: see text] We report here the development of two different sensing strategies based on the use of antigen-conjugated nucleic acid strands for the detection of a bispecific antibody against the tumor-related proteins Mucin1 and epidermal growth factor receptor. Both approaches work well in serum samples (nanomolar sensitivity), show high specificity against the two monospecific antibodies, and are rapid. The results presented here demonstrate the versatility of DNA-based platforms for the detection of bispecific antibodies and could represent a versatile alternative to other more reagent-intensive and time-consuming analytical approaches.
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spelling pubmed-106835032023-11-30 Bispecific Antibody Detection Using Antigen-Conjugated Synthetic Nucleic Acid Strands Mariottini, Davide Bracaglia, Sara Barbero, Luca Fuchs, Sebastian W. Saal, Christoph Moniot, Sebastien Knuehl, Christine Baranda, Lorena Ranallo, Simona Ricci, Francesco ACS Sens [Image: see text] We report here the development of two different sensing strategies based on the use of antigen-conjugated nucleic acid strands for the detection of a bispecific antibody against the tumor-related proteins Mucin1 and epidermal growth factor receptor. Both approaches work well in serum samples (nanomolar sensitivity), show high specificity against the two monospecific antibodies, and are rapid. The results presented here demonstrate the versatility of DNA-based platforms for the detection of bispecific antibodies and could represent a versatile alternative to other more reagent-intensive and time-consuming analytical approaches. American Chemical Society 2023-10-19 /pmc/articles/PMC10683503/ /pubmed/37856082 http://dx.doi.org/10.1021/acssensors.3c01717 Text en © 2023 The Authors. Published by American Chemical Society https://creativecommons.org/licenses/by/4.0/Permits the broadest form of re-use including for commercial purposes, provided that author attribution and integrity are maintained (https://creativecommons.org/licenses/by/4.0/).
spellingShingle Mariottini, Davide
Bracaglia, Sara
Barbero, Luca
Fuchs, Sebastian W.
Saal, Christoph
Moniot, Sebastien
Knuehl, Christine
Baranda, Lorena
Ranallo, Simona
Ricci, Francesco
Bispecific Antibody Detection Using Antigen-Conjugated Synthetic Nucleic Acid Strands
title Bispecific Antibody Detection Using Antigen-Conjugated Synthetic Nucleic Acid Strands
title_full Bispecific Antibody Detection Using Antigen-Conjugated Synthetic Nucleic Acid Strands
title_fullStr Bispecific Antibody Detection Using Antigen-Conjugated Synthetic Nucleic Acid Strands
title_full_unstemmed Bispecific Antibody Detection Using Antigen-Conjugated Synthetic Nucleic Acid Strands
title_short Bispecific Antibody Detection Using Antigen-Conjugated Synthetic Nucleic Acid Strands
title_sort bispecific antibody detection using antigen-conjugated synthetic nucleic acid strands
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10683503/
https://www.ncbi.nlm.nih.gov/pubmed/37856082
http://dx.doi.org/10.1021/acssensors.3c01717
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