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Effects of Blue Light on Puberty and Ovary in Female Rats

OBJECTIVE: This study was designed to examine the effect of blue light exposure and exposure time on puberty in an animal model. METHODS: Eighteen 21-day-old female Sprague Dawley rats were divided into three equal groups which were: control group (CG); blue light-6 hours (BL-6); and blue light-12 h...

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Autores principales: Kılınç Uğurlu, Aylin, Bideci, Aysun, Demirel, Mürşide Ayşe, Take Kaplanoğlu, Gülnur, Dayanır, Duygu, Gülbahar, Özlem, Deveci Bulut, Tuba Saadet, Döğer, Esra, Çamurdan, Mahmut Orhun
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Galenos Publishing 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10683537/
https://www.ncbi.nlm.nih.gov/pubmed/37212628
http://dx.doi.org/10.4274/jcrpe.galenos.2023.2022-12-1
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author Kılınç Uğurlu, Aylin
Bideci, Aysun
Demirel, Mürşide Ayşe
Take Kaplanoğlu, Gülnur
Dayanır, Duygu
Gülbahar, Özlem
Deveci Bulut, Tuba Saadet
Döğer, Esra
Çamurdan, Mahmut Orhun
author_facet Kılınç Uğurlu, Aylin
Bideci, Aysun
Demirel, Mürşide Ayşe
Take Kaplanoğlu, Gülnur
Dayanır, Duygu
Gülbahar, Özlem
Deveci Bulut, Tuba Saadet
Döğer, Esra
Çamurdan, Mahmut Orhun
author_sort Kılınç Uğurlu, Aylin
collection PubMed
description OBJECTIVE: This study was designed to examine the effect of blue light exposure and exposure time on puberty in an animal model. METHODS: Eighteen 21-day-old female Sprague Dawley rats were divided into three equal groups which were: control group (CG); blue light-6 hours (BL-6); and blue light-12 hours (BL-12). CG rats were maintained with 12/12-hour light-dark cycles. The animals in BL-6 and BL-12 were exposed to blue light of wavelength 450-470 nm and intensity of 0.03 uW/cm(2) for 6 and 12 hours, respectively. Exposure to blue light continued until the first signs of puberty. Serum follicle stimulating hormone (FSH), luteinizing hormone (LH), estradiol, testosterone, dehydroepiandrosterone sulfate (DHEA-S), leptin and melatonin were measured. Subsequently the ovaries and uterus were examined histomorphologically. RESULTS: The median day of puberty start was 38, 32 and 30 for the CG, BL-6, and BL-12 groups, respectively (p=0.001). FSH, testosterone, DHEA-S, and leptin concentrations of all groups were similar. However, LH and estradiol concentrations in BL-6 were higher compared to CG (p=0.02). There was a negative correlation between blue light exposure, exposure time, and melatonin concentrations (r=-0.537, p=0.048). Ovarian tissue was compatible with puberty in all groups. As blue light exposure time increased, capillary dilatation and edema in the ovarian tissue increased. Prolonged exposure was associated with polycystic ovary-like (PCO) morphological changes and apoptosis in granulosa cells. CONCLUSION: These results suggest that exposure to blue light and the duration of exposure induced earlier puberty in female rats. As the duration of blue light exposure increased, PCO-like inflammation, and apoptosis were detected in the ovaries.
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spelling pubmed-106835372023-12-01 Effects of Blue Light on Puberty and Ovary in Female Rats Kılınç Uğurlu, Aylin Bideci, Aysun Demirel, Mürşide Ayşe Take Kaplanoğlu, Gülnur Dayanır, Duygu Gülbahar, Özlem Deveci Bulut, Tuba Saadet Döğer, Esra Çamurdan, Mahmut Orhun J Clin Res Pediatr Endocrinol Original Article OBJECTIVE: This study was designed to examine the effect of blue light exposure and exposure time on puberty in an animal model. METHODS: Eighteen 21-day-old female Sprague Dawley rats were divided into three equal groups which were: control group (CG); blue light-6 hours (BL-6); and blue light-12 hours (BL-12). CG rats were maintained with 12/12-hour light-dark cycles. The animals in BL-6 and BL-12 were exposed to blue light of wavelength 450-470 nm and intensity of 0.03 uW/cm(2) for 6 and 12 hours, respectively. Exposure to blue light continued until the first signs of puberty. Serum follicle stimulating hormone (FSH), luteinizing hormone (LH), estradiol, testosterone, dehydroepiandrosterone sulfate (DHEA-S), leptin and melatonin were measured. Subsequently the ovaries and uterus were examined histomorphologically. RESULTS: The median day of puberty start was 38, 32 and 30 for the CG, BL-6, and BL-12 groups, respectively (p=0.001). FSH, testosterone, DHEA-S, and leptin concentrations of all groups were similar. However, LH and estradiol concentrations in BL-6 were higher compared to CG (p=0.02). There was a negative correlation between blue light exposure, exposure time, and melatonin concentrations (r=-0.537, p=0.048). Ovarian tissue was compatible with puberty in all groups. As blue light exposure time increased, capillary dilatation and edema in the ovarian tissue increased. Prolonged exposure was associated with polycystic ovary-like (PCO) morphological changes and apoptosis in granulosa cells. CONCLUSION: These results suggest that exposure to blue light and the duration of exposure induced earlier puberty in female rats. As the duration of blue light exposure increased, PCO-like inflammation, and apoptosis were detected in the ovaries. Galenos Publishing 2023-12 2023-11-22 /pmc/articles/PMC10683537/ /pubmed/37212628 http://dx.doi.org/10.4274/jcrpe.galenos.2023.2022-12-1 Text en ©Copyright 2023 by Turkish Pediatric Endocrinology and Diabetes Society | The Journal of Clinical Research in Pediatric Endocrinology published by Galenos Publishing House. https://creativecommons.org/licenses/by-nc-nd/4.0/Licensed under a Creative Commons Attribution-NonCommercial-NoDerivatives 4.0 (CC BY-NC-ND) International License.
spellingShingle Original Article
Kılınç Uğurlu, Aylin
Bideci, Aysun
Demirel, Mürşide Ayşe
Take Kaplanoğlu, Gülnur
Dayanır, Duygu
Gülbahar, Özlem
Deveci Bulut, Tuba Saadet
Döğer, Esra
Çamurdan, Mahmut Orhun
Effects of Blue Light on Puberty and Ovary in Female Rats
title Effects of Blue Light on Puberty and Ovary in Female Rats
title_full Effects of Blue Light on Puberty and Ovary in Female Rats
title_fullStr Effects of Blue Light on Puberty and Ovary in Female Rats
title_full_unstemmed Effects of Blue Light on Puberty and Ovary in Female Rats
title_short Effects of Blue Light on Puberty and Ovary in Female Rats
title_sort effects of blue light on puberty and ovary in female rats
topic Original Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10683537/
https://www.ncbi.nlm.nih.gov/pubmed/37212628
http://dx.doi.org/10.4274/jcrpe.galenos.2023.2022-12-1
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