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Immunohistochemical expression of E-Cadherin and Cyclin D1 in different grades of oral squamous cell carcinoma
BACKGROUND: Oral squamous cell carcinoma (OSCC) is the most common oral malignancy, representing up to 80–90% of all malignant neoplasms of the oral cavity. It results from the multistep accumulation of heterogeneous genetic changes. Important risk factors for OSCC include the use of tobacco or bete...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Wolters Kluwer - Medknow
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10683894/ https://www.ncbi.nlm.nih.gov/pubmed/38033971 http://dx.doi.org/10.4103/jomfp.jomfp_257_23 |
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author | Dar, Mohammad S. Abbas, Rezhat Shah, Zeenat Latoo, Suheel H. Gowhar, Owais |
author_facet | Dar, Mohammad S. Abbas, Rezhat Shah, Zeenat Latoo, Suheel H. Gowhar, Owais |
author_sort | Dar, Mohammad S. |
collection | PubMed |
description | BACKGROUND: Oral squamous cell carcinoma (OSCC) is the most common oral malignancy, representing up to 80–90% of all malignant neoplasms of the oral cavity. It results from the multistep accumulation of heterogeneous genetic changes. Important risk factors for OSCC include the use of tobacco or betel quid chewing, alcohol consumption, human papillomavirus and poor nutrition. E-Cadherin as a tumour suppressor gene sets a threshold for Wnt/β-catenin signalling. When expression of E-Cadherin is lost, potentiation of Wnt signalling pathway occurs leading to loss of cell–cell adhesion. The cyclin D1 gene (CCND1) located on chromosome 11q13 encodes a nuclear protein that is the regulatory subunit of Cdk-4 and Cdk-6. Cyclin D1 plays a major role in cell cycle transition from G1 to S phase by contributing to inactivation of the retinoblastoma gene product, and overexpression of CCND1 has been reported in 35–40% cases of OSCC. AIM: Considering this, we decided to evaluate and compare the expression of CE-Cadherin and Cyclin D1 in different grades of OSCC. MATERIALS AND METHODS: A retrospective study was carried out on 60 formalin-fixed paraffin embedded tissue blocks comprising of 20 cases of well-differentiated OSCC, 20 cases of moderately differentiated OSCC and 20 cases of poorly differentiated OSCC. Diagnosed (using H and E), with oral mucosa taken as control. RESULTS: There was downregulation of E-Cadherin and overexpression of Cyclin D1 in increasing grades of OSCC and the difference was statistically significant. E-Cadherin was localised to membranous and shifted to cytoplasm as the grade worsened. Cyclin D1 was localised to nuclei of cells and the expression was seen more at the peripheral portions of tumour islands depicting the proliferative activity of tumour front. CONCLUSION: The study revealed a good prognostic role of both E-Cadherin and Cyclin D1 in OSCC. The markers can be used for prognostic as well as therapeutic purposes. |
format | Online Article Text |
id | pubmed-10683894 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Wolters Kluwer - Medknow |
record_format | MEDLINE/PubMed |
spelling | pubmed-106838942023-11-30 Immunohistochemical expression of E-Cadherin and Cyclin D1 in different grades of oral squamous cell carcinoma Dar, Mohammad S. Abbas, Rezhat Shah, Zeenat Latoo, Suheel H. Gowhar, Owais J Oral Maxillofac Pathol Original Article BACKGROUND: Oral squamous cell carcinoma (OSCC) is the most common oral malignancy, representing up to 80–90% of all malignant neoplasms of the oral cavity. It results from the multistep accumulation of heterogeneous genetic changes. Important risk factors for OSCC include the use of tobacco or betel quid chewing, alcohol consumption, human papillomavirus and poor nutrition. E-Cadherin as a tumour suppressor gene sets a threshold for Wnt/β-catenin signalling. When expression of E-Cadherin is lost, potentiation of Wnt signalling pathway occurs leading to loss of cell–cell adhesion. The cyclin D1 gene (CCND1) located on chromosome 11q13 encodes a nuclear protein that is the regulatory subunit of Cdk-4 and Cdk-6. Cyclin D1 plays a major role in cell cycle transition from G1 to S phase by contributing to inactivation of the retinoblastoma gene product, and overexpression of CCND1 has been reported in 35–40% cases of OSCC. AIM: Considering this, we decided to evaluate and compare the expression of CE-Cadherin and Cyclin D1 in different grades of OSCC. MATERIALS AND METHODS: A retrospective study was carried out on 60 formalin-fixed paraffin embedded tissue blocks comprising of 20 cases of well-differentiated OSCC, 20 cases of moderately differentiated OSCC and 20 cases of poorly differentiated OSCC. Diagnosed (using H and E), with oral mucosa taken as control. RESULTS: There was downregulation of E-Cadherin and overexpression of Cyclin D1 in increasing grades of OSCC and the difference was statistically significant. E-Cadherin was localised to membranous and shifted to cytoplasm as the grade worsened. Cyclin D1 was localised to nuclei of cells and the expression was seen more at the peripheral portions of tumour islands depicting the proliferative activity of tumour front. CONCLUSION: The study revealed a good prognostic role of both E-Cadherin and Cyclin D1 in OSCC. The markers can be used for prognostic as well as therapeutic purposes. Wolters Kluwer - Medknow 2023 2023-09-12 /pmc/articles/PMC10683894/ /pubmed/38033971 http://dx.doi.org/10.4103/jomfp.jomfp_257_23 Text en Copyright: © 2023 Journal of Oral and Maxillofacial Pathology https://creativecommons.org/licenses/by-nc-sa/4.0/This is an open access journal, and articles are distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 4.0 License, which allows others to remix, tweak, and build upon the work non-commercially, as long as appropriate credit is given and the new creations are licensed under the identical terms. |
spellingShingle | Original Article Dar, Mohammad S. Abbas, Rezhat Shah, Zeenat Latoo, Suheel H. Gowhar, Owais Immunohistochemical expression of E-Cadherin and Cyclin D1 in different grades of oral squamous cell carcinoma |
title | Immunohistochemical expression of E-Cadherin and Cyclin D1 in different grades of oral squamous cell carcinoma |
title_full | Immunohistochemical expression of E-Cadherin and Cyclin D1 in different grades of oral squamous cell carcinoma |
title_fullStr | Immunohistochemical expression of E-Cadherin and Cyclin D1 in different grades of oral squamous cell carcinoma |
title_full_unstemmed | Immunohistochemical expression of E-Cadherin and Cyclin D1 in different grades of oral squamous cell carcinoma |
title_short | Immunohistochemical expression of E-Cadherin and Cyclin D1 in different grades of oral squamous cell carcinoma |
title_sort | immunohistochemical expression of e-cadherin and cyclin d1 in different grades of oral squamous cell carcinoma |
topic | Original Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10683894/ https://www.ncbi.nlm.nih.gov/pubmed/38033971 http://dx.doi.org/10.4103/jomfp.jomfp_257_23 |
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