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Carboxyl nanodiamonds inhibit melanoma tumor metastases by blocking cellular motility and invasiveness

Carboxyl nanodiamond (cND) nanoparticles are actively internalized by B16F10 melanoma cells in culture. Treatment of B16F10 tumor cells with cNDs in vitro inhibited their ability to (i) migrate and invade through porous membranes in a transwell culture system, (ii) secrete matrix metalloproteinases...

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Autores principales: Behera, Sushreesangita P, Tyagi, Witty, Saxena, Rajiv K
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10683945/
https://www.ncbi.nlm.nih.gov/pubmed/38034091
http://dx.doi.org/10.1093/pnasnexus/pgad359
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author Behera, Sushreesangita P
Tyagi, Witty
Saxena, Rajiv K
author_facet Behera, Sushreesangita P
Tyagi, Witty
Saxena, Rajiv K
author_sort Behera, Sushreesangita P
collection PubMed
description Carboxyl nanodiamond (cND) nanoparticles are actively internalized by B16F10 melanoma cells in culture. Treatment of B16F10 tumor cells with cNDs in vitro inhibited their ability to (i) migrate and invade through porous membranes in a transwell culture system, (ii) secrete matrix metalloproteinases (MMPs) MMP-2 and MMP-9, and (iii) express selected epithelial–mesenchymal transition markers critical for cell migration and invasion. Administration of luciferase-transfected B16F10-Luc2 melanoma cells resulted in a rapid growth of the tumor and its metastasis to different organs that could be monitored by in vivo bioluminescence imaging as well as by ex vivo BLI of the mouse organs. After tumor cells were administered intravenously in C57Bl/6 mice, administration of cNDs (50 μg i.v. every alternate day) resulted in marked suppression of the tumor growth and metastasis in different organs of mice. Subcutaneous administration of B16F10 cells resulted in robust growth of the primary tumor subcutaneously as well as its metastasis to the lungs, liver, spleen, and kidneys. Intravenous treatment with cNDs did not affect the growth of the primary tumor mass but essentially blocked the metastasis of the tumor to different organs. Histological examination of mouse organs indicated that the administration of cNDs by itself was safe and did not cause toxic changes in mouse organs. These results indicate that the cND treatment may have an antimetastatic effect on the spread of B16F10 melanoma tumor cells in mice. Further exploration of cNDs as a possible antimetastatic therapeutic agent is suggested.
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spelling pubmed-106839452023-11-30 Carboxyl nanodiamonds inhibit melanoma tumor metastases by blocking cellular motility and invasiveness Behera, Sushreesangita P Tyagi, Witty Saxena, Rajiv K PNAS Nexus Biological, Health, and Medical Sciences Carboxyl nanodiamond (cND) nanoparticles are actively internalized by B16F10 melanoma cells in culture. Treatment of B16F10 tumor cells with cNDs in vitro inhibited their ability to (i) migrate and invade through porous membranes in a transwell culture system, (ii) secrete matrix metalloproteinases (MMPs) MMP-2 and MMP-9, and (iii) express selected epithelial–mesenchymal transition markers critical for cell migration and invasion. Administration of luciferase-transfected B16F10-Luc2 melanoma cells resulted in a rapid growth of the tumor and its metastasis to different organs that could be monitored by in vivo bioluminescence imaging as well as by ex vivo BLI of the mouse organs. After tumor cells were administered intravenously in C57Bl/6 mice, administration of cNDs (50 μg i.v. every alternate day) resulted in marked suppression of the tumor growth and metastasis in different organs of mice. Subcutaneous administration of B16F10 cells resulted in robust growth of the primary tumor subcutaneously as well as its metastasis to the lungs, liver, spleen, and kidneys. Intravenous treatment with cNDs did not affect the growth of the primary tumor mass but essentially blocked the metastasis of the tumor to different organs. Histological examination of mouse organs indicated that the administration of cNDs by itself was safe and did not cause toxic changes in mouse organs. These results indicate that the cND treatment may have an antimetastatic effect on the spread of B16F10 melanoma tumor cells in mice. Further exploration of cNDs as a possible antimetastatic therapeutic agent is suggested. Oxford University Press 2023-11-28 /pmc/articles/PMC10683945/ /pubmed/38034091 http://dx.doi.org/10.1093/pnasnexus/pgad359 Text en © The Author(s) 2023. Published by Oxford University Press on behalf of National Academy of Sciences. https://creativecommons.org/licenses/by-nc-nd/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs licence (https://creativecommons.org/licenses/by-nc-nd/4.0/), which permits non-commercial reproduction and distribution of the work, in any medium, provided the original work is not altered or transformed in any way, and that the work is properly cited. For commercial re-use, please contact journals.permissions@oup.com
spellingShingle Biological, Health, and Medical Sciences
Behera, Sushreesangita P
Tyagi, Witty
Saxena, Rajiv K
Carboxyl nanodiamonds inhibit melanoma tumor metastases by blocking cellular motility and invasiveness
title Carboxyl nanodiamonds inhibit melanoma tumor metastases by blocking cellular motility and invasiveness
title_full Carboxyl nanodiamonds inhibit melanoma tumor metastases by blocking cellular motility and invasiveness
title_fullStr Carboxyl nanodiamonds inhibit melanoma tumor metastases by blocking cellular motility and invasiveness
title_full_unstemmed Carboxyl nanodiamonds inhibit melanoma tumor metastases by blocking cellular motility and invasiveness
title_short Carboxyl nanodiamonds inhibit melanoma tumor metastases by blocking cellular motility and invasiveness
title_sort carboxyl nanodiamonds inhibit melanoma tumor metastases by blocking cellular motility and invasiveness
topic Biological, Health, and Medical Sciences
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10683945/
https://www.ncbi.nlm.nih.gov/pubmed/38034091
http://dx.doi.org/10.1093/pnasnexus/pgad359
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