selSeq: A method for the enrichment of non-polyadenylated RNAs including enhancer and long non-coding RNAs for sequencing

Non-polyadenylated RNA includes a large subset of crucial regulators of RNA expression and constitutes a substantial portion of the transcriptome, playing essential roles in gene regulation. For example, enhancer RNAs are long non-coding RNAs that perform enhancer-like functions, are bi-directionall...

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Autores principales: Limberis, Jason D., Nalyvayko, Alina, Ernst, Joel D., Metcalfe, John Z.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Public Library of Science 2023
Materias:
Lab Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10684010/
https://www.ncbi.nlm.nih.gov/pubmed/38015898
http://dx.doi.org/10.1371/journal.pone.0289442
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author Limberis, Jason D.
Nalyvayko, Alina
Ernst, Joel D.
Metcalfe, John Z.
author_facet Limberis, Jason D.
Nalyvayko, Alina
Ernst, Joel D.
Metcalfe, John Z.
author_sort Limberis, Jason D.
collection PubMed
description Non-polyadenylated RNA includes a large subset of crucial regulators of RNA expression and constitutes a substantial portion of the transcriptome, playing essential roles in gene regulation. For example, enhancer RNAs are long non-coding RNAs that perform enhancer-like functions, are bi-directionally transcribed, and usually lack polyA tails. This paper presents a novel method, selSeq, that selectively removes mRNA and pre-mRNA from samples enabling the selective sequencing of crucial regulatory elements, including non-polyadenylated RNAs such as long non-coding RNA, enhancer RNA, and non-canonical mRNA.
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spelling pubmed-106840102023-11-30 selSeq: A method for the enrichment of non-polyadenylated RNAs including enhancer and long non-coding RNAs for sequencing Limberis, Jason D. Nalyvayko, Alina Ernst, Joel D. Metcalfe, John Z. PLoS One Lab Protocol Non-polyadenylated RNA includes a large subset of crucial regulators of RNA expression and constitutes a substantial portion of the transcriptome, playing essential roles in gene regulation. For example, enhancer RNAs are long non-coding RNAs that perform enhancer-like functions, are bi-directionally transcribed, and usually lack polyA tails. This paper presents a novel method, selSeq, that selectively removes mRNA and pre-mRNA from samples enabling the selective sequencing of crucial regulatory elements, including non-polyadenylated RNAs such as long non-coding RNA, enhancer RNA, and non-canonical mRNA. Public Library of Science 2023-11-28 /pmc/articles/PMC10684010/ /pubmed/38015898 http://dx.doi.org/10.1371/journal.pone.0289442 Text en © 2023 Limberis et al https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
spellingShingle Lab Protocol
Limberis, Jason D.
Nalyvayko, Alina
Ernst, Joel D.
Metcalfe, John Z.
selSeq: A method for the enrichment of non-polyadenylated RNAs including enhancer and long non-coding RNAs for sequencing
title selSeq: A method for the enrichment of non-polyadenylated RNAs including enhancer and long non-coding RNAs for sequencing
title_full selSeq: A method for the enrichment of non-polyadenylated RNAs including enhancer and long non-coding RNAs for sequencing
title_fullStr selSeq: A method for the enrichment of non-polyadenylated RNAs including enhancer and long non-coding RNAs for sequencing
title_full_unstemmed selSeq: A method for the enrichment of non-polyadenylated RNAs including enhancer and long non-coding RNAs for sequencing
title_short selSeq: A method for the enrichment of non-polyadenylated RNAs including enhancer and long non-coding RNAs for sequencing
title_sort selseq: a method for the enrichment of non-polyadenylated rnas including enhancer and long non-coding rnas for sequencing
topic Lab Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10684010/
https://www.ncbi.nlm.nih.gov/pubmed/38015898
http://dx.doi.org/10.1371/journal.pone.0289442
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