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Inhibitory effects of polysaccharides from Korean ginseng berries on LPS-induced RAW264.7 macrophages
Polysaccharides isolated from Korean ginseng berries (GBPs) have shown beneficial effects such as immunomodulatory, anti-inflammatory, anti-cancer, and anti-diabetic properties. However, little is known about anti-inflammatory effects of GBPs. Thus, the purpose of this study was to investigate anti-...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Public Library of Science
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10684074/ https://www.ncbi.nlm.nih.gov/pubmed/38015971 http://dx.doi.org/10.1371/journal.pone.0294675 |
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author | Rod-in, Weerawan Surayot, Utoomporn You, SangGuan Park, Woo Jung |
author_facet | Rod-in, Weerawan Surayot, Utoomporn You, SangGuan Park, Woo Jung |
author_sort | Rod-in, Weerawan |
collection | PubMed |
description | Polysaccharides isolated from Korean ginseng berries (GBPs) have shown beneficial effects such as immunomodulatory, anti-inflammatory, anti-cancer, and anti-diabetic properties. However, little is known about anti-inflammatory effects of GBPs. Thus, the purpose of this study was to investigate anti-inflammatory properties of four fractions of GBPs, namely GBP-C, GBP-F1, GBP-F2, and GBP-F3, in macrophages. Their toxicities and effects on NO production in RAW264.7 cells were assessed by culturing cells with various concentrations of GBPs and stimulating cells with LPS. Furthermore, expression levels of inflammatory mediators, cytokines, cell surface molecules, and immune signaling pathways were evaluated in LPS-stimulated macrophages using different fractions of GBPs at 450 μg/mL. These GBPs activated LPS-stimulated RAW264.7 cells to significantly reduce NO production. They suppressed the expression of mRNA and cell surface molecules via MAPK and NF-κB pathways. Collectively, results revealed that all four GBP fractions showed anti-inflammatory effects, with GBP-F1 having a more efficient anti-inflammatory effect than GBP-C, GBP-F2, and GBP-F3. The structure of GBP-F1 mainly consists of 1 → 3)- Araf, 1 → 4)- Glcp, and 1 → 6)–Galp glycosidic linkages. These results demonstrate that GBPs can be employed as alternative natural sources of anti-inflammatory agents. |
format | Online Article Text |
id | pubmed-10684074 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Public Library of Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-106840742023-11-30 Inhibitory effects of polysaccharides from Korean ginseng berries on LPS-induced RAW264.7 macrophages Rod-in, Weerawan Surayot, Utoomporn You, SangGuan Park, Woo Jung PLoS One Research Article Polysaccharides isolated from Korean ginseng berries (GBPs) have shown beneficial effects such as immunomodulatory, anti-inflammatory, anti-cancer, and anti-diabetic properties. However, little is known about anti-inflammatory effects of GBPs. Thus, the purpose of this study was to investigate anti-inflammatory properties of four fractions of GBPs, namely GBP-C, GBP-F1, GBP-F2, and GBP-F3, in macrophages. Their toxicities and effects on NO production in RAW264.7 cells were assessed by culturing cells with various concentrations of GBPs and stimulating cells with LPS. Furthermore, expression levels of inflammatory mediators, cytokines, cell surface molecules, and immune signaling pathways were evaluated in LPS-stimulated macrophages using different fractions of GBPs at 450 μg/mL. These GBPs activated LPS-stimulated RAW264.7 cells to significantly reduce NO production. They suppressed the expression of mRNA and cell surface molecules via MAPK and NF-κB pathways. Collectively, results revealed that all four GBP fractions showed anti-inflammatory effects, with GBP-F1 having a more efficient anti-inflammatory effect than GBP-C, GBP-F2, and GBP-F3. The structure of GBP-F1 mainly consists of 1 → 3)- Araf, 1 → 4)- Glcp, and 1 → 6)–Galp glycosidic linkages. These results demonstrate that GBPs can be employed as alternative natural sources of anti-inflammatory agents. Public Library of Science 2023-11-28 /pmc/articles/PMC10684074/ /pubmed/38015971 http://dx.doi.org/10.1371/journal.pone.0294675 Text en © 2023 Rod-in et al https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. |
spellingShingle | Research Article Rod-in, Weerawan Surayot, Utoomporn You, SangGuan Park, Woo Jung Inhibitory effects of polysaccharides from Korean ginseng berries on LPS-induced RAW264.7 macrophages |
title | Inhibitory effects of polysaccharides from Korean ginseng berries on LPS-induced RAW264.7 macrophages |
title_full | Inhibitory effects of polysaccharides from Korean ginseng berries on LPS-induced RAW264.7 macrophages |
title_fullStr | Inhibitory effects of polysaccharides from Korean ginseng berries on LPS-induced RAW264.7 macrophages |
title_full_unstemmed | Inhibitory effects of polysaccharides from Korean ginseng berries on LPS-induced RAW264.7 macrophages |
title_short | Inhibitory effects of polysaccharides from Korean ginseng berries on LPS-induced RAW264.7 macrophages |
title_sort | inhibitory effects of polysaccharides from korean ginseng berries on lps-induced raw264.7 macrophages |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10684074/ https://www.ncbi.nlm.nih.gov/pubmed/38015971 http://dx.doi.org/10.1371/journal.pone.0294675 |
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