Cargando…

Saccharomyces cerevisiae additions normalized hemocyte differential genes expression and regulated crayfish (Procambarus clarkii) oxidative damage under cadmium stress

Because China produces the most crayfish in the world, safe solutions must be improved to mitigate the risks of ongoing heavy metal stressors accumulation. This study aimed to use Saccharomyces cerevisiae as a bioremediation agent to counteract the harmful effect of cadmium (Cd) on crayfish (Procamb...

Descripción completa

Detalles Bibliográficos
Autores principales: Yang, Yaru, Li, Shuaidong, Zhu, Yumin, Che, Litao, Wu, Qifan, Bai, Shijun, Shu, Guocheng, Zhao, Xianming, Guo, Peng, Soaud, Salma A., Li, Nianzhen, Deng, Mengling, Li, Jia, El-Sappah, Ahmed H.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10684557/
https://www.ncbi.nlm.nih.gov/pubmed/38016989
http://dx.doi.org/10.1038/s41598-023-47323-1
_version_ 1785151427722608640
author Yang, Yaru
Li, Shuaidong
Zhu, Yumin
Che, Litao
Wu, Qifan
Bai, Shijun
Shu, Guocheng
Zhao, Xianming
Guo, Peng
Soaud, Salma A.
Li, Nianzhen
Deng, Mengling
Li, Jia
El-Sappah, Ahmed H.
author_facet Yang, Yaru
Li, Shuaidong
Zhu, Yumin
Che, Litao
Wu, Qifan
Bai, Shijun
Shu, Guocheng
Zhao, Xianming
Guo, Peng
Soaud, Salma A.
Li, Nianzhen
Deng, Mengling
Li, Jia
El-Sappah, Ahmed H.
author_sort Yang, Yaru
collection PubMed
description Because China produces the most crayfish in the world, safe solutions must be improved to mitigate the risks of ongoing heavy metal stressors accumulation. This study aimed to use Saccharomyces cerevisiae as a bioremediation agent to counteract the harmful effect of cadmium (Cd) on crayfish (Procambarus clarkia). Our study used three concentrations of S. cerevisiae on crayfish feed to assess their Cd toxicity remediation effect by measuring total antioxidant capacity (TAC) and the biomarkers related to oxidative stress like malondialdehyde (MDA), protein carbonyl derivates (PCO), and DNA–protein crosslink (DPC). A graphite furnace atomic absorption spectroscopy device was used to determine Cd contents in crayfish. Furthermore, the mRNA expression levels of lysozyme (LSZ), metallothionein (MT), and prophenoloxidase (proPO) were evaluated before and following the addition of S. cerevisiae. The results indicated that S. cerevisae at 5% supplemented in fundamental feed exhibited the best removal effect, and Cd removal rates at days 4th, 8th, 12th, and 21st were 12, 19, 29.7, and 66.45%, respectively, which were significantly higher than the basal diet of crayfish. The addition of S. cerevisiae increased TAC levels. On the other hand, it decreased MDA, PCO, and DPC, which had risen due to Cd exposure. Furthermore, it increased the expression of proPO, which was reduced by Cd exposure, and decreased the expression of LSZ and MT, acting in the opposite direction of Cd exposure alone. These findings demonstrated that feeding S. cerevisiae effectively reduces the Cd from crayfish and could be used to develop Cd-free crayfish-based foods.
format Online
Article
Text
id pubmed-10684557
institution National Center for Biotechnology Information
language English
publishDate 2023
publisher Nature Publishing Group UK
record_format MEDLINE/PubMed
spelling pubmed-106845572023-11-30 Saccharomyces cerevisiae additions normalized hemocyte differential genes expression and regulated crayfish (Procambarus clarkii) oxidative damage under cadmium stress Yang, Yaru Li, Shuaidong Zhu, Yumin Che, Litao Wu, Qifan Bai, Shijun Shu, Guocheng Zhao, Xianming Guo, Peng Soaud, Salma A. Li, Nianzhen Deng, Mengling Li, Jia El-Sappah, Ahmed H. Sci Rep Article Because China produces the most crayfish in the world, safe solutions must be improved to mitigate the risks of ongoing heavy metal stressors accumulation. This study aimed to use Saccharomyces cerevisiae as a bioremediation agent to counteract the harmful effect of cadmium (Cd) on crayfish (Procambarus clarkia). Our study used three concentrations of S. cerevisiae on crayfish feed to assess their Cd toxicity remediation effect by measuring total antioxidant capacity (TAC) and the biomarkers related to oxidative stress like malondialdehyde (MDA), protein carbonyl derivates (PCO), and DNA–protein crosslink (DPC). A graphite furnace atomic absorption spectroscopy device was used to determine Cd contents in crayfish. Furthermore, the mRNA expression levels of lysozyme (LSZ), metallothionein (MT), and prophenoloxidase (proPO) were evaluated before and following the addition of S. cerevisiae. The results indicated that S. cerevisae at 5% supplemented in fundamental feed exhibited the best removal effect, and Cd removal rates at days 4th, 8th, 12th, and 21st were 12, 19, 29.7, and 66.45%, respectively, which were significantly higher than the basal diet of crayfish. The addition of S. cerevisiae increased TAC levels. On the other hand, it decreased MDA, PCO, and DPC, which had risen due to Cd exposure. Furthermore, it increased the expression of proPO, which was reduced by Cd exposure, and decreased the expression of LSZ and MT, acting in the opposite direction of Cd exposure alone. These findings demonstrated that feeding S. cerevisiae effectively reduces the Cd from crayfish and could be used to develop Cd-free crayfish-based foods. Nature Publishing Group UK 2023-11-28 /pmc/articles/PMC10684557/ /pubmed/38016989 http://dx.doi.org/10.1038/s41598-023-47323-1 Text en © The Author(s) 2023 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Article
Yang, Yaru
Li, Shuaidong
Zhu, Yumin
Che, Litao
Wu, Qifan
Bai, Shijun
Shu, Guocheng
Zhao, Xianming
Guo, Peng
Soaud, Salma A.
Li, Nianzhen
Deng, Mengling
Li, Jia
El-Sappah, Ahmed H.
Saccharomyces cerevisiae additions normalized hemocyte differential genes expression and regulated crayfish (Procambarus clarkii) oxidative damage under cadmium stress
title Saccharomyces cerevisiae additions normalized hemocyte differential genes expression and regulated crayfish (Procambarus clarkii) oxidative damage under cadmium stress
title_full Saccharomyces cerevisiae additions normalized hemocyte differential genes expression and regulated crayfish (Procambarus clarkii) oxidative damage under cadmium stress
title_fullStr Saccharomyces cerevisiae additions normalized hemocyte differential genes expression and regulated crayfish (Procambarus clarkii) oxidative damage under cadmium stress
title_full_unstemmed Saccharomyces cerevisiae additions normalized hemocyte differential genes expression and regulated crayfish (Procambarus clarkii) oxidative damage under cadmium stress
title_short Saccharomyces cerevisiae additions normalized hemocyte differential genes expression and regulated crayfish (Procambarus clarkii) oxidative damage under cadmium stress
title_sort saccharomyces cerevisiae additions normalized hemocyte differential genes expression and regulated crayfish (procambarus clarkii) oxidative damage under cadmium stress
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10684557/
https://www.ncbi.nlm.nih.gov/pubmed/38016989
http://dx.doi.org/10.1038/s41598-023-47323-1
work_keys_str_mv AT yangyaru saccharomycescerevisiaeadditionsnormalizedhemocytedifferentialgenesexpressionandregulatedcrayfishprocambarusclarkiioxidativedamageundercadmiumstress
AT lishuaidong saccharomycescerevisiaeadditionsnormalizedhemocytedifferentialgenesexpressionandregulatedcrayfishprocambarusclarkiioxidativedamageundercadmiumstress
AT zhuyumin saccharomycescerevisiaeadditionsnormalizedhemocytedifferentialgenesexpressionandregulatedcrayfishprocambarusclarkiioxidativedamageundercadmiumstress
AT chelitao saccharomycescerevisiaeadditionsnormalizedhemocytedifferentialgenesexpressionandregulatedcrayfishprocambarusclarkiioxidativedamageundercadmiumstress
AT wuqifan saccharomycescerevisiaeadditionsnormalizedhemocytedifferentialgenesexpressionandregulatedcrayfishprocambarusclarkiioxidativedamageundercadmiumstress
AT baishijun saccharomycescerevisiaeadditionsnormalizedhemocytedifferentialgenesexpressionandregulatedcrayfishprocambarusclarkiioxidativedamageundercadmiumstress
AT shuguocheng saccharomycescerevisiaeadditionsnormalizedhemocytedifferentialgenesexpressionandregulatedcrayfishprocambarusclarkiioxidativedamageundercadmiumstress
AT zhaoxianming saccharomycescerevisiaeadditionsnormalizedhemocytedifferentialgenesexpressionandregulatedcrayfishprocambarusclarkiioxidativedamageundercadmiumstress
AT guopeng saccharomycescerevisiaeadditionsnormalizedhemocytedifferentialgenesexpressionandregulatedcrayfishprocambarusclarkiioxidativedamageundercadmiumstress
AT soaudsalmaa saccharomycescerevisiaeadditionsnormalizedhemocytedifferentialgenesexpressionandregulatedcrayfishprocambarusclarkiioxidativedamageundercadmiumstress
AT linianzhen saccharomycescerevisiaeadditionsnormalizedhemocytedifferentialgenesexpressionandregulatedcrayfishprocambarusclarkiioxidativedamageundercadmiumstress
AT dengmengling saccharomycescerevisiaeadditionsnormalizedhemocytedifferentialgenesexpressionandregulatedcrayfishprocambarusclarkiioxidativedamageundercadmiumstress
AT lijia saccharomycescerevisiaeadditionsnormalizedhemocytedifferentialgenesexpressionandregulatedcrayfishprocambarusclarkiioxidativedamageundercadmiumstress
AT elsappahahmedh saccharomycescerevisiaeadditionsnormalizedhemocytedifferentialgenesexpressionandregulatedcrayfishprocambarusclarkiioxidativedamageundercadmiumstress