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Massive and efficient encapsulation of single cells in monodisperse droplets and collagen–alginate microgels using a microfluidic device

Single-cell manipulation is the key foundation of life exploration at individual cell resolution. Constructing easy-to-use, high-throughput, and biomimetic manipulative tools for efficient single-cell operation is quite necessary. In this study, a facile and efficient encapsulation of single cells r...

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Autores principales: Liu, Dan, Xuanyuan, Tingting, Liu, Xufang, Fu, Wenzhu, Liu, Wenming
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10684782/
https://www.ncbi.nlm.nih.gov/pubmed/38033813
http://dx.doi.org/10.3389/fbioe.2023.1281375
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author Liu, Dan
Xuanyuan, Tingting
Liu, Xufang
Fu, Wenzhu
Liu, Wenming
author_facet Liu, Dan
Xuanyuan, Tingting
Liu, Xufang
Fu, Wenzhu
Liu, Wenming
author_sort Liu, Dan
collection PubMed
description Single-cell manipulation is the key foundation of life exploration at individual cell resolution. Constructing easy-to-use, high-throughput, and biomimetic manipulative tools for efficient single-cell operation is quite necessary. In this study, a facile and efficient encapsulation of single cells relying on the massive and controllable production of droplets and collagen–alginate microgels using a microfluidic device is presented. High monodispersity and geometric homogeneity of both droplet and microgel generation were experimentally demonstrated based on the well-investigated microfluidic fabricating procedure. The reliability of the microfluidic platform for controllable, high-throughput, and improved single-cell encapsulation in monodisperse droplets and microgels was also confirmed. A single-cell encapsulation rate of up to 33.6% was achieved based on the established microfluidic operation. The introduction of stromal material in droplets/microgels for encapsulation provided single cells an in vivo simulated microenvironment. The single-cell operation achievement offers a methodological approach for developing simple and miniaturized devices to perform single-cell manipulation and analysis in a high-throughput and microenvironment-biomimetic manner. We believe that it holds great potential for applications in precision medicine, cell microengineering, drug discovery, and biosensing.
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spelling pubmed-106847822023-11-30 Massive and efficient encapsulation of single cells in monodisperse droplets and collagen–alginate microgels using a microfluidic device Liu, Dan Xuanyuan, Tingting Liu, Xufang Fu, Wenzhu Liu, Wenming Front Bioeng Biotechnol Bioengineering and Biotechnology Single-cell manipulation is the key foundation of life exploration at individual cell resolution. Constructing easy-to-use, high-throughput, and biomimetic manipulative tools for efficient single-cell operation is quite necessary. In this study, a facile and efficient encapsulation of single cells relying on the massive and controllable production of droplets and collagen–alginate microgels using a microfluidic device is presented. High monodispersity and geometric homogeneity of both droplet and microgel generation were experimentally demonstrated based on the well-investigated microfluidic fabricating procedure. The reliability of the microfluidic platform for controllable, high-throughput, and improved single-cell encapsulation in monodisperse droplets and microgels was also confirmed. A single-cell encapsulation rate of up to 33.6% was achieved based on the established microfluidic operation. The introduction of stromal material in droplets/microgels for encapsulation provided single cells an in vivo simulated microenvironment. The single-cell operation achievement offers a methodological approach for developing simple and miniaturized devices to perform single-cell manipulation and analysis in a high-throughput and microenvironment-biomimetic manner. We believe that it holds great potential for applications in precision medicine, cell microengineering, drug discovery, and biosensing. Frontiers Media S.A. 2023-11-15 /pmc/articles/PMC10684782/ /pubmed/38033813 http://dx.doi.org/10.3389/fbioe.2023.1281375 Text en Copyright © 2023 Liu, Xuanyuan, Liu, Fu and Liu. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Bioengineering and Biotechnology
Liu, Dan
Xuanyuan, Tingting
Liu, Xufang
Fu, Wenzhu
Liu, Wenming
Massive and efficient encapsulation of single cells in monodisperse droplets and collagen–alginate microgels using a microfluidic device
title Massive and efficient encapsulation of single cells in monodisperse droplets and collagen–alginate microgels using a microfluidic device
title_full Massive and efficient encapsulation of single cells in monodisperse droplets and collagen–alginate microgels using a microfluidic device
title_fullStr Massive and efficient encapsulation of single cells in monodisperse droplets and collagen–alginate microgels using a microfluidic device
title_full_unstemmed Massive and efficient encapsulation of single cells in monodisperse droplets and collagen–alginate microgels using a microfluidic device
title_short Massive and efficient encapsulation of single cells in monodisperse droplets and collagen–alginate microgels using a microfluidic device
title_sort massive and efficient encapsulation of single cells in monodisperse droplets and collagen–alginate microgels using a microfluidic device
topic Bioengineering and Biotechnology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10684782/
https://www.ncbi.nlm.nih.gov/pubmed/38033813
http://dx.doi.org/10.3389/fbioe.2023.1281375
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