Protocol for CRISPR-Cas9-mediated genome editing to study spermatogenesis in Caenorhabditis elegans

Gene silencing by P-element-induced wimpy testis-interacting RNAs is a mechanism to maintain genome integrity in germ cells. Here, we present a protocol for knockin or knockout editing of male germline genome mediated by CRISPR-Cas9 technology in Caenorhabditis elegans. We describe steps for constru...

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Detalles Bibliográficos
Autores principales: Wang, Peng, Cao, Zheng, Wang, Qiushi, Ma, Xiaojing, Wang, Ning, Chen, Lianwan, Zhao, Yanmei, Miao, Long
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10684875/
https://www.ncbi.nlm.nih.gov/pubmed/37967017
http://dx.doi.org/10.1016/j.xpro.2023.102720

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