Protocol for performing consecutive bone marrow transplants in mice to study the role of marrow niche in supporting hematopoiesis

Hematopoietic stem and progenitor cells depend on bone marrow (BM) stromal cells for survival. Here, we present a protocol for performing three consecutive BM transplants in mice to study the role of BM niche in supporting hematopoiesis. We describe steps for transplanting cells to condition the mar...

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Detalles Bibliográficos
Autores principales: Gopal, Aparna, Ibrahim, Rawa, Karsan, Aly
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Protocol
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10684876/
https://www.ncbi.nlm.nih.gov/pubmed/37967015
http://dx.doi.org/10.1016/j.xpro.2023.102719
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author Gopal, Aparna
Ibrahim, Rawa
Karsan, Aly
author_facet Gopal, Aparna
Ibrahim, Rawa
Karsan, Aly
author_sort Gopal, Aparna
collection PubMed
description Hematopoietic stem and progenitor cells depend on bone marrow (BM) stromal cells for survival. Here, we present a protocol for performing three consecutive BM transplants in mice to study the role of BM niche in supporting hematopoiesis. We describe steps for transplanting cells to condition the marrow of the recipient mice and transplanting wild-type cells to examine the effect of the conditioned marrow in supporting hematopoiesis. We then detail procedures for transplanting into wild-type recipients to measure bone marrow chimerism. For complete details on the use and execution of this protocol, please refer to Gopal et al. (2022).(1)
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spelling pubmed-106848762023-11-30 Protocol for performing consecutive bone marrow transplants in mice to study the role of marrow niche in supporting hematopoiesis Gopal, Aparna Ibrahim, Rawa Karsan, Aly STAR Protoc Protocol Hematopoietic stem and progenitor cells depend on bone marrow (BM) stromal cells for survival. Here, we present a protocol for performing three consecutive BM transplants in mice to study the role of BM niche in supporting hematopoiesis. We describe steps for transplanting cells to condition the marrow of the recipient mice and transplanting wild-type cells to examine the effect of the conditioned marrow in supporting hematopoiesis. We then detail procedures for transplanting into wild-type recipients to measure bone marrow chimerism. For complete details on the use and execution of this protocol, please refer to Gopal et al. (2022).(1) Elsevier 2023-11-17 /pmc/articles/PMC10684876/ /pubmed/37967015 http://dx.doi.org/10.1016/j.xpro.2023.102719 Text en © 2023 The Authors. https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Protocol
Gopal, Aparna
Ibrahim, Rawa
Karsan, Aly
Protocol for performing consecutive bone marrow transplants in mice to study the role of marrow niche in supporting hematopoiesis
title Protocol for performing consecutive bone marrow transplants in mice to study the role of marrow niche in supporting hematopoiesis
title_full Protocol for performing consecutive bone marrow transplants in mice to study the role of marrow niche in supporting hematopoiesis
title_fullStr Protocol for performing consecutive bone marrow transplants in mice to study the role of marrow niche in supporting hematopoiesis
title_full_unstemmed Protocol for performing consecutive bone marrow transplants in mice to study the role of marrow niche in supporting hematopoiesis
title_short Protocol for performing consecutive bone marrow transplants in mice to study the role of marrow niche in supporting hematopoiesis
title_sort protocol for performing consecutive bone marrow transplants in mice to study the role of marrow niche in supporting hematopoiesis
topic Protocol
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10684876/
https://www.ncbi.nlm.nih.gov/pubmed/37967015
http://dx.doi.org/10.1016/j.xpro.2023.102719
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