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Chromosome-level changes and genome elimination by manipulation of CENH3 in carrot (Daucus carota)

Hybrid cultivars are valuable in many crop species due to their high yield, uniformity, and other desirable traits. Doubled haploids, which have two identical sets of chromosomes, are valuable for hybrid breeding because they can be produced in one generation, in comparison to the multigenerational...

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Autores principales: Meyer, Chandler M., Goldman, Irwin L., Krysan, Patrick J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10684906/
https://www.ncbi.nlm.nih.gov/pubmed/38034555
http://dx.doi.org/10.3389/fpls.2023.1294551
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author Meyer, Chandler M.
Goldman, Irwin L.
Krysan, Patrick J.
author_facet Meyer, Chandler M.
Goldman, Irwin L.
Krysan, Patrick J.
author_sort Meyer, Chandler M.
collection PubMed
description Hybrid cultivars are valuable in many crop species due to their high yield, uniformity, and other desirable traits. Doubled haploids, which have two identical sets of chromosomes, are valuable for hybrid breeding because they can be produced in one generation, in comparison to the multigenerational process typically used to produce inbred parents for hybrid production. One method to produce haploid plants is manipulation of centromeric histone H3 (CENH3). This method of producing haploids has so far been successful in Arabidopsis, maize (Zea mays), and wheat (Triticum aestivum). Here we describe modification of CENH3 in carrot (Daucus carota) to test for the ability of these modifications to induce uniparental genome elimination, which is the basis for haploid induction. Base editing was used to make cenh3 mutant plants with amino acid substitutions in the region of CENH3 encoding the histone fold domain. These cenh3 mutant plants were then outcrossed with CENH3 wild-type plants. Using PCR-based genotyping assays, we identified two candidates for genome elimination. One candidate was classified as a putative aneuploid plant in which chromosome 7 is in a single copy state. The other candidate was characterized as a putative tetraploid that was likely haploid during its genesis. Our results suggest that this putative tetraploid inherited all of its chromosomes from the CENH3 wild-type parent and that the genome of the cenh3 mutant plant was lost. This study provides evidence that modification of CENH3 in carrot has the potential to induce genome elimination and ploidy changes in carrot.
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spelling pubmed-106849062023-11-30 Chromosome-level changes and genome elimination by manipulation of CENH3 in carrot (Daucus carota) Meyer, Chandler M. Goldman, Irwin L. Krysan, Patrick J. Front Plant Sci Plant Science Hybrid cultivars are valuable in many crop species due to their high yield, uniformity, and other desirable traits. Doubled haploids, which have two identical sets of chromosomes, are valuable for hybrid breeding because they can be produced in one generation, in comparison to the multigenerational process typically used to produce inbred parents for hybrid production. One method to produce haploid plants is manipulation of centromeric histone H3 (CENH3). This method of producing haploids has so far been successful in Arabidopsis, maize (Zea mays), and wheat (Triticum aestivum). Here we describe modification of CENH3 in carrot (Daucus carota) to test for the ability of these modifications to induce uniparental genome elimination, which is the basis for haploid induction. Base editing was used to make cenh3 mutant plants with amino acid substitutions in the region of CENH3 encoding the histone fold domain. These cenh3 mutant plants were then outcrossed with CENH3 wild-type plants. Using PCR-based genotyping assays, we identified two candidates for genome elimination. One candidate was classified as a putative aneuploid plant in which chromosome 7 is in a single copy state. The other candidate was characterized as a putative tetraploid that was likely haploid during its genesis. Our results suggest that this putative tetraploid inherited all of its chromosomes from the CENH3 wild-type parent and that the genome of the cenh3 mutant plant was lost. This study provides evidence that modification of CENH3 in carrot has the potential to induce genome elimination and ploidy changes in carrot. Frontiers Media S.A. 2023-11-15 /pmc/articles/PMC10684906/ /pubmed/38034555 http://dx.doi.org/10.3389/fpls.2023.1294551 Text en Copyright © 2023 Meyer, Goldman and Krysan https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Plant Science
Meyer, Chandler M.
Goldman, Irwin L.
Krysan, Patrick J.
Chromosome-level changes and genome elimination by manipulation of CENH3 in carrot (Daucus carota)
title Chromosome-level changes and genome elimination by manipulation of CENH3 in carrot (Daucus carota)
title_full Chromosome-level changes and genome elimination by manipulation of CENH3 in carrot (Daucus carota)
title_fullStr Chromosome-level changes and genome elimination by manipulation of CENH3 in carrot (Daucus carota)
title_full_unstemmed Chromosome-level changes and genome elimination by manipulation of CENH3 in carrot (Daucus carota)
title_short Chromosome-level changes and genome elimination by manipulation of CENH3 in carrot (Daucus carota)
title_sort chromosome-level changes and genome elimination by manipulation of cenh3 in carrot (daucus carota)
topic Plant Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10684906/
https://www.ncbi.nlm.nih.gov/pubmed/38034555
http://dx.doi.org/10.3389/fpls.2023.1294551
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