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miR-460b-5p promotes proliferation and differentiation of chicken myoblasts and targets RBM19 gene
The meat production of broilers is crucial to economic benefits of broiler industries, while the slaughter performance of broilers is directly determined by skeletal muscle development. Hence, the broiler breeding for growth traits shows a great importance. As a kind of small noncoding RNA, microRNA...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10685028/ https://www.ncbi.nlm.nih.gov/pubmed/37980764 http://dx.doi.org/10.1016/j.psj.2023.103231 |
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author | Zhou, Kai-Zhi Wu, Peng-Fei Ling, Xuan-Ze Zhang, Jin Wang, Qi-Fan Zhang, Xin-Chao Xue, Qian Zhang, Tao Han, Wei Zhang, Gen-Xi |
author_facet | Zhou, Kai-Zhi Wu, Peng-Fei Ling, Xuan-Ze Zhang, Jin Wang, Qi-Fan Zhang, Xin-Chao Xue, Qian Zhang, Tao Han, Wei Zhang, Gen-Xi |
author_sort | Zhou, Kai-Zhi |
collection | PubMed |
description | The meat production of broilers is crucial to economic benefits of broiler industries, while the slaughter performance of broilers is directly determined by skeletal muscle development. Hence, the broiler breeding for growth traits shows a great importance. As a kind of small noncoding RNA, microRNA (miRNA) can regulate the expression of multiple genes and perform a wide range of regulation in organisms. Currently, more and more studies have confirmed that miRNAs are closely associated with skeletal muscle development of chickens. Based on our previous miR-seq analysis (accession number: PRJNA668199), miR-460b-5p was screened as one of the key miRNAs probably involved in the growth regulation of chickens. However, the regulatory effect of miR-460b-5p on the development of chicken skeletal muscles is still unclear. Therefore, miR-460b-5p was further used for functional validation at the cellular level in this study. The expression pattern of miR-460b-5p was investigated in proliferation and differentiation stages of chicken primary myoblasts. It was showed that the expression level of miR-460b-5p gradually decreased from the proliferation stage (GM 50%) to the lowest at 24 h of differentiation. As differentiation proceeded, miR-460b-5p expression increased significantly, reaching the highest and stabilizing at 72 h and 96 h of differentiation. Through mRNA quantitative analysis of proliferation marker genes, CCK-8 and Edu assays, miR-460b-5p was found to significantly facilitate the transition of myoblasts from G1 to S phase and promote chicken myoblast proliferation. mRNA and protein quantitative analysis of differentiation marker genes, as well as the indirect immunofluorescence results of myotubes, revealed that miR-460b-5p significantly stimulated myotube development and promote chicken myoblast differentiation. In addition, the target relationship was validated for miR-460b-5p according to the dual-luciferase reporter assay and mRNA quantitative analysis, which indicates that miR-460b-5p was able to regulate RBM19 expression by specifically binding to the 3′ UTR of RBM19. In summary, miR-460b-5p has positive regulatory effects on the proliferation and differentiation of chicken myoblasts, and RBM19 is a target gene of miR-460b-5p. |
format | Online Article Text |
id | pubmed-10685028 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Elsevier |
record_format | MEDLINE/PubMed |
spelling | pubmed-106850282023-11-30 miR-460b-5p promotes proliferation and differentiation of chicken myoblasts and targets RBM19 gene Zhou, Kai-Zhi Wu, Peng-Fei Ling, Xuan-Ze Zhang, Jin Wang, Qi-Fan Zhang, Xin-Chao Xue, Qian Zhang, Tao Han, Wei Zhang, Gen-Xi Poult Sci GENETICS AND MOLECULAR BIOLOGY The meat production of broilers is crucial to economic benefits of broiler industries, while the slaughter performance of broilers is directly determined by skeletal muscle development. Hence, the broiler breeding for growth traits shows a great importance. As a kind of small noncoding RNA, microRNA (miRNA) can regulate the expression of multiple genes and perform a wide range of regulation in organisms. Currently, more and more studies have confirmed that miRNAs are closely associated with skeletal muscle development of chickens. Based on our previous miR-seq analysis (accession number: PRJNA668199), miR-460b-5p was screened as one of the key miRNAs probably involved in the growth regulation of chickens. However, the regulatory effect of miR-460b-5p on the development of chicken skeletal muscles is still unclear. Therefore, miR-460b-5p was further used for functional validation at the cellular level in this study. The expression pattern of miR-460b-5p was investigated in proliferation and differentiation stages of chicken primary myoblasts. It was showed that the expression level of miR-460b-5p gradually decreased from the proliferation stage (GM 50%) to the lowest at 24 h of differentiation. As differentiation proceeded, miR-460b-5p expression increased significantly, reaching the highest and stabilizing at 72 h and 96 h of differentiation. Through mRNA quantitative analysis of proliferation marker genes, CCK-8 and Edu assays, miR-460b-5p was found to significantly facilitate the transition of myoblasts from G1 to S phase and promote chicken myoblast proliferation. mRNA and protein quantitative analysis of differentiation marker genes, as well as the indirect immunofluorescence results of myotubes, revealed that miR-460b-5p significantly stimulated myotube development and promote chicken myoblast differentiation. In addition, the target relationship was validated for miR-460b-5p according to the dual-luciferase reporter assay and mRNA quantitative analysis, which indicates that miR-460b-5p was able to regulate RBM19 expression by specifically binding to the 3′ UTR of RBM19. In summary, miR-460b-5p has positive regulatory effects on the proliferation and differentiation of chicken myoblasts, and RBM19 is a target gene of miR-460b-5p. Elsevier 2023-10-24 /pmc/articles/PMC10685028/ /pubmed/37980764 http://dx.doi.org/10.1016/j.psj.2023.103231 Text en © 2023 The Authors https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
spellingShingle | GENETICS AND MOLECULAR BIOLOGY Zhou, Kai-Zhi Wu, Peng-Fei Ling, Xuan-Ze Zhang, Jin Wang, Qi-Fan Zhang, Xin-Chao Xue, Qian Zhang, Tao Han, Wei Zhang, Gen-Xi miR-460b-5p promotes proliferation and differentiation of chicken myoblasts and targets RBM19 gene |
title | miR-460b-5p promotes proliferation and differentiation of chicken myoblasts and targets RBM19 gene |
title_full | miR-460b-5p promotes proliferation and differentiation of chicken myoblasts and targets RBM19 gene |
title_fullStr | miR-460b-5p promotes proliferation and differentiation of chicken myoblasts and targets RBM19 gene |
title_full_unstemmed | miR-460b-5p promotes proliferation and differentiation of chicken myoblasts and targets RBM19 gene |
title_short | miR-460b-5p promotes proliferation and differentiation of chicken myoblasts and targets RBM19 gene |
title_sort | mir-460b-5p promotes proliferation and differentiation of chicken myoblasts and targets rbm19 gene |
topic | GENETICS AND MOLECULAR BIOLOGY |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10685028/ https://www.ncbi.nlm.nih.gov/pubmed/37980764 http://dx.doi.org/10.1016/j.psj.2023.103231 |
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