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Evaluation of oxidative stress in an experimental model of Crohn's disease treated with hyperbaric oxygen therapy

Introduction: Treatments of Inflammatory Bowel Disease (IBD) are able to control symptoms in most cases, however, a fraction of patients do not improve or have a loss of response to treatments, making it important to explore new therapeutic strategies. Hyperbaric oxygen therapy (HBO) may represent o...

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Autores principales: Nakutis, Fernanda Serafim, Nishitokukado, Iêda, dos Santos, Fabiana Maria, Ortiz-Agostinho, Carmen Lucia, de Alencar, Daniel Teixeira, Achtschin, Cassiana Ganem, Nunes, Valeria Sutti, Leite, André Zonetti Arruda, Sipahi, Aytan Miranda
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hospital das Clinicas da Faculdade de Medicina da Universidade de Sao Paulo 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10685139/
https://www.ncbi.nlm.nih.gov/pubmed/37976650
http://dx.doi.org/10.1016/j.clinsp.2023.100305
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author Nakutis, Fernanda Serafim
Nishitokukado, Iêda
dos Santos, Fabiana Maria
Ortiz-Agostinho, Carmen Lucia
de Alencar, Daniel Teixeira
Achtschin, Cassiana Ganem
Nunes, Valeria Sutti
Leite, André Zonetti Arruda
Sipahi, Aytan Miranda
author_facet Nakutis, Fernanda Serafim
Nishitokukado, Iêda
dos Santos, Fabiana Maria
Ortiz-Agostinho, Carmen Lucia
de Alencar, Daniel Teixeira
Achtschin, Cassiana Ganem
Nunes, Valeria Sutti
Leite, André Zonetti Arruda
Sipahi, Aytan Miranda
author_sort Nakutis, Fernanda Serafim
collection PubMed
description Introduction: Treatments of Inflammatory Bowel Disease (IBD) are able to control symptoms in most cases, however, a fraction of patients do not improve or have a loss of response to treatments, making it important to explore new therapeutic strategies. Hyperbaric oxygen therapy (HBO) may represent one of them. The aim of this study was to evaluate the effects of HBO therapy in an experimental model of IBD. Methods: Sixty male BALBc mice were divided into six groups. Group 1 was colitis-induced with trinitrobenzene sulfonic acid (TNBS) + ethanol, group 2 received TNBS + ethanol plus HBO, group 3 received only ethanol, group 4 received ethanol plus HBO, group 5 received saline solution, and group 6 received saline solution plus HBO. HBO was performed for four days, subsequently, the mice were evaluated daily. At the end of the study, samples from the intestine were collected for histological analysis as well as for measurement of antioxidant enzymes and cytokine levels. Results: HBO significantly improved the clinical and histological status of the animals. Treatment with HBO increased the activity of the antioxidant enzymes superoxide dismutase (SOD) and glutathione peroxidase (GPx) in all of the groups; moreover, the difference was only significant between the TNBS and TNBS + HBO groups and treatments promoted a reduction in the proinflammatory cytokines IFN-γ, IL-12, IL-17 and TNF-α and increased the anti-inflammatory cytokines IL-4 and IL-10, with no changes in IL-13. Conclusion: HBO effectively treats TNBS-induced colitis by increasing the activity of antioxidant enzymes and modulating cytokine profiles.
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spelling pubmed-106851392023-11-30 Evaluation of oxidative stress in an experimental model of Crohn's disease treated with hyperbaric oxygen therapy Nakutis, Fernanda Serafim Nishitokukado, Iêda dos Santos, Fabiana Maria Ortiz-Agostinho, Carmen Lucia de Alencar, Daniel Teixeira Achtschin, Cassiana Ganem Nunes, Valeria Sutti Leite, André Zonetti Arruda Sipahi, Aytan Miranda Clinics (Sao Paulo) Original Articles Introduction: Treatments of Inflammatory Bowel Disease (IBD) are able to control symptoms in most cases, however, a fraction of patients do not improve or have a loss of response to treatments, making it important to explore new therapeutic strategies. Hyperbaric oxygen therapy (HBO) may represent one of them. The aim of this study was to evaluate the effects of HBO therapy in an experimental model of IBD. Methods: Sixty male BALBc mice were divided into six groups. Group 1 was colitis-induced with trinitrobenzene sulfonic acid (TNBS) + ethanol, group 2 received TNBS + ethanol plus HBO, group 3 received only ethanol, group 4 received ethanol plus HBO, group 5 received saline solution, and group 6 received saline solution plus HBO. HBO was performed for four days, subsequently, the mice were evaluated daily. At the end of the study, samples from the intestine were collected for histological analysis as well as for measurement of antioxidant enzymes and cytokine levels. Results: HBO significantly improved the clinical and histological status of the animals. Treatment with HBO increased the activity of the antioxidant enzymes superoxide dismutase (SOD) and glutathione peroxidase (GPx) in all of the groups; moreover, the difference was only significant between the TNBS and TNBS + HBO groups and treatments promoted a reduction in the proinflammatory cytokines IFN-γ, IL-12, IL-17 and TNF-α and increased the anti-inflammatory cytokines IL-4 and IL-10, with no changes in IL-13. Conclusion: HBO effectively treats TNBS-induced colitis by increasing the activity of antioxidant enzymes and modulating cytokine profiles. Hospital das Clinicas da Faculdade de Medicina da Universidade de Sao Paulo 2023-11-15 /pmc/articles/PMC10685139/ /pubmed/37976650 http://dx.doi.org/10.1016/j.clinsp.2023.100305 Text en © 2023 HCFMUSP. Published by Elsevier España, S.L.U. https://creativecommons.org/licenses/by/4.0/This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Original Articles
Nakutis, Fernanda Serafim
Nishitokukado, Iêda
dos Santos, Fabiana Maria
Ortiz-Agostinho, Carmen Lucia
de Alencar, Daniel Teixeira
Achtschin, Cassiana Ganem
Nunes, Valeria Sutti
Leite, André Zonetti Arruda
Sipahi, Aytan Miranda
Evaluation of oxidative stress in an experimental model of Crohn's disease treated with hyperbaric oxygen therapy
title Evaluation of oxidative stress in an experimental model of Crohn's disease treated with hyperbaric oxygen therapy
title_full Evaluation of oxidative stress in an experimental model of Crohn's disease treated with hyperbaric oxygen therapy
title_fullStr Evaluation of oxidative stress in an experimental model of Crohn's disease treated with hyperbaric oxygen therapy
title_full_unstemmed Evaluation of oxidative stress in an experimental model of Crohn's disease treated with hyperbaric oxygen therapy
title_short Evaluation of oxidative stress in an experimental model of Crohn's disease treated with hyperbaric oxygen therapy
title_sort evaluation of oxidative stress in an experimental model of crohn's disease treated with hyperbaric oxygen therapy
topic Original Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10685139/
https://www.ncbi.nlm.nih.gov/pubmed/37976650
http://dx.doi.org/10.1016/j.clinsp.2023.100305
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