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Quantitative mass spectrometric analysis of hepatocellular carcinoma biomarker alpha-fetoprotein
Serum alpha-fetoprotein (AFP) has been used as a marker for the diagnosis of hepatocellular carcinoma (HCC) and its core fucosylation is associated with the early stage of HCC. However, current methods for the detection of AFP with core fucose are not highly accurate for early diagnosis. In this stu...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
RSC
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10685801/ https://www.ncbi.nlm.nih.gov/pubmed/38033722 http://dx.doi.org/10.1039/d3cb00069a |
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author | Chen, Chen-Chun Huang, Han-Wen Chen, Bo-Rui Wong, Chi-Huey |
author_facet | Chen, Chen-Chun Huang, Han-Wen Chen, Bo-Rui Wong, Chi-Huey |
author_sort | Chen, Chen-Chun |
collection | PubMed |
description | Serum alpha-fetoprotein (AFP) has been used as a marker for the diagnosis of hepatocellular carcinoma (HCC) and its core fucosylation is associated with the early stage of HCC. However, current methods for the detection of AFP with core fucose are not highly accurate for early diagnosis. In this study, we established an enzyme-assisted mass spectrometric method for the quantitative analysis of AFP/core fucose with high specificity and sensitivity. We employed endoglycosidase treatment of AFP to improve the biomarker analysis. The accuracy and precision are within the US FDA-suggested value, and a good linearity (r(2) = 0.9930) and a detection limit of 15.6 ng mL(−1) can be achieved. |
format | Online Article Text |
id | pubmed-10685801 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | RSC |
record_format | MEDLINE/PubMed |
spelling | pubmed-106858012023-11-30 Quantitative mass spectrometric analysis of hepatocellular carcinoma biomarker alpha-fetoprotein Chen, Chen-Chun Huang, Han-Wen Chen, Bo-Rui Wong, Chi-Huey RSC Chem Biol Chemistry Serum alpha-fetoprotein (AFP) has been used as a marker for the diagnosis of hepatocellular carcinoma (HCC) and its core fucosylation is associated with the early stage of HCC. However, current methods for the detection of AFP with core fucose are not highly accurate for early diagnosis. In this study, we established an enzyme-assisted mass spectrometric method for the quantitative analysis of AFP/core fucose with high specificity and sensitivity. We employed endoglycosidase treatment of AFP to improve the biomarker analysis. The accuracy and precision are within the US FDA-suggested value, and a good linearity (r(2) = 0.9930) and a detection limit of 15.6 ng mL(−1) can be achieved. RSC 2023-10-09 /pmc/articles/PMC10685801/ /pubmed/38033722 http://dx.doi.org/10.1039/d3cb00069a Text en This journal is © The Royal Society of Chemistry https://creativecommons.org/licenses/by-nc/3.0/ |
spellingShingle | Chemistry Chen, Chen-Chun Huang, Han-Wen Chen, Bo-Rui Wong, Chi-Huey Quantitative mass spectrometric analysis of hepatocellular carcinoma biomarker alpha-fetoprotein |
title | Quantitative mass spectrometric analysis of hepatocellular carcinoma biomarker alpha-fetoprotein |
title_full | Quantitative mass spectrometric analysis of hepatocellular carcinoma biomarker alpha-fetoprotein |
title_fullStr | Quantitative mass spectrometric analysis of hepatocellular carcinoma biomarker alpha-fetoprotein |
title_full_unstemmed | Quantitative mass spectrometric analysis of hepatocellular carcinoma biomarker alpha-fetoprotein |
title_short | Quantitative mass spectrometric analysis of hepatocellular carcinoma biomarker alpha-fetoprotein |
title_sort | quantitative mass spectrometric analysis of hepatocellular carcinoma biomarker alpha-fetoprotein |
topic | Chemistry |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10685801/ https://www.ncbi.nlm.nih.gov/pubmed/38033722 http://dx.doi.org/10.1039/d3cb00069a |
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