Cargando…

Inflammatory Bowel Disease Through the Lens of Single-cell RNA-seq Technologies

The intestinal mucosa represents a unique environment where the coordinated function of diverse epithelial, mesenchymal, and immune cells maintains a physiologically balanced environment in the presence of gut microbiota. The intestinal mucosa plays a central role in the pathogenesis of inflammatory...

Descripción completa

Detalles Bibliográficos
Autores principales: Corridoni, Daniele, Chapman, Thomas, Antanaviciute, Agne, Satsangi, Jack, Simmons, Alison
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10686606/
https://www.ncbi.nlm.nih.gov/pubmed/32386055
http://dx.doi.org/10.1093/ibd/izaa089
_version_ 1785151810600697856
author Corridoni, Daniele
Chapman, Thomas
Antanaviciute, Agne
Satsangi, Jack
Simmons, Alison
author_facet Corridoni, Daniele
Chapman, Thomas
Antanaviciute, Agne
Satsangi, Jack
Simmons, Alison
author_sort Corridoni, Daniele
collection PubMed
description The intestinal mucosa represents a unique environment where the coordinated function of diverse epithelial, mesenchymal, and immune cells maintains a physiologically balanced environment in the presence of gut microbiota. The intestinal mucosa plays a central role in the pathogenesis of inflammatory bowel disease (IBD), yet the molecular and cellular composition of this diverse environment is poorly understood. However, the recent advent of multimodal single-cell technologies, including single-cell RNA sequencing (scRNA-seq), now provides an opportunity to accurately map the tissue architecture, characterize rare cell types that were previously overlooked, and define function at a single-cell level. In this review, we summarize key advances in single-cell technology and provide an overview of important aspects of computational analysis. We describe emerging data in the field of IBD and discuss how the characterization of novel intestinal mucosa cell populations is reshaping our understanding of this complex disease. We conclude by considering the potential clinical applications, including the definition of novel drug targets and the opportunity for personalization of care in this exciting new era of precision medicine.
format Online
Article
Text
id pubmed-10686606
institution National Center for Biotechnology Information
language English
publishDate 2020
publisher Oxford University Press
record_format MEDLINE/PubMed
spelling pubmed-106866062023-11-30 Inflammatory Bowel Disease Through the Lens of Single-cell RNA-seq Technologies Corridoni, Daniele Chapman, Thomas Antanaviciute, Agne Satsangi, Jack Simmons, Alison Inflamm Bowel Dis Basic Science Review The intestinal mucosa represents a unique environment where the coordinated function of diverse epithelial, mesenchymal, and immune cells maintains a physiologically balanced environment in the presence of gut microbiota. The intestinal mucosa plays a central role in the pathogenesis of inflammatory bowel disease (IBD), yet the molecular and cellular composition of this diverse environment is poorly understood. However, the recent advent of multimodal single-cell technologies, including single-cell RNA sequencing (scRNA-seq), now provides an opportunity to accurately map the tissue architecture, characterize rare cell types that were previously overlooked, and define function at a single-cell level. In this review, we summarize key advances in single-cell technology and provide an overview of important aspects of computational analysis. We describe emerging data in the field of IBD and discuss how the characterization of novel intestinal mucosa cell populations is reshaping our understanding of this complex disease. We conclude by considering the potential clinical applications, including the definition of novel drug targets and the opportunity for personalization of care in this exciting new era of precision medicine. Oxford University Press 2020-05-09 /pmc/articles/PMC10686606/ /pubmed/32386055 http://dx.doi.org/10.1093/ibd/izaa089 Text en © 2020 Crohn’s & Colitis Foundation. Published by Oxford University Press on behalf of Crohn’s & Colitis Foundation. https://creativecommons.org/licenses/by/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Basic Science Review
Corridoni, Daniele
Chapman, Thomas
Antanaviciute, Agne
Satsangi, Jack
Simmons, Alison
Inflammatory Bowel Disease Through the Lens of Single-cell RNA-seq Technologies
title Inflammatory Bowel Disease Through the Lens of Single-cell RNA-seq Technologies
title_full Inflammatory Bowel Disease Through the Lens of Single-cell RNA-seq Technologies
title_fullStr Inflammatory Bowel Disease Through the Lens of Single-cell RNA-seq Technologies
title_full_unstemmed Inflammatory Bowel Disease Through the Lens of Single-cell RNA-seq Technologies
title_short Inflammatory Bowel Disease Through the Lens of Single-cell RNA-seq Technologies
title_sort inflammatory bowel disease through the lens of single-cell rna-seq technologies
topic Basic Science Review
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10686606/
https://www.ncbi.nlm.nih.gov/pubmed/32386055
http://dx.doi.org/10.1093/ibd/izaa089
work_keys_str_mv AT corridonidaniele inflammatoryboweldiseasethroughthelensofsinglecellrnaseqtechnologies
AT chapmanthomas inflammatoryboweldiseasethroughthelensofsinglecellrnaseqtechnologies
AT antanaviciuteagne inflammatoryboweldiseasethroughthelensofsinglecellrnaseqtechnologies
AT satsangijack inflammatoryboweldiseasethroughthelensofsinglecellrnaseqtechnologies
AT simmonsalison inflammatoryboweldiseasethroughthelensofsinglecellrnaseqtechnologies