Illuminating the mechanism and allosteric behavior of NanoLuc luciferase

NanoLuc, a superior β-barrel fold luciferase, was engineered 10 years ago but the nature of its catalysis remains puzzling. Here experimental and computational techniques are combined, revealing that imidazopyrazinone luciferins bind to an intra-barrel catalytic site but also to an allosteric site s...

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Detalles Bibliográficos
Autores principales: Nemergut, Michal, Pluskal, Daniel, Horackova, Jana, Sustrova, Tereza, Tulis, Jan, Barta, Tomas, Baatallah, Racha, Gagnot, Glwadys, Novakova, Veronika, Majerova, Marika, Sedlackova, Karolina, Marques, Sérgio M., Toul, Martin, Damborsky, Jiri, Prokop, Zbynek, Bednar, David, Janin, Yves L., Marek, Martin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2023
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10687086/
https://www.ncbi.nlm.nih.gov/pubmed/38030625
http://dx.doi.org/10.1038/s41467-023-43403-y

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