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Tetraploid embryo aggregation produces high-quality blastocysts with an increased trophectoderm in pigs
Tetraploid complementation is an ideal method for demonstrating the differentiation potential of pluripotent stem cells. In this study, we selected the most efficient tetraploid production method for porcine embryos and investigated whether tetraploid blastomere aggregation could enhance the quality...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Frontiers Media S.A.
2023
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10687364/ https://www.ncbi.nlm.nih.gov/pubmed/38033873 http://dx.doi.org/10.3389/fcell.2023.1239448 |
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author | Lee, Joohyeong Cai, Lian Kim, Mirae Choi, Hyerin Oh, Dongjin Jawad, Ali Lee, Eunsong Hyun, Sang-Hwan |
author_facet | Lee, Joohyeong Cai, Lian Kim, Mirae Choi, Hyerin Oh, Dongjin Jawad, Ali Lee, Eunsong Hyun, Sang-Hwan |
author_sort | Lee, Joohyeong |
collection | PubMed |
description | Tetraploid complementation is an ideal method for demonstrating the differentiation potential of pluripotent stem cells. In this study, we selected the most efficient tetraploid production method for porcine embryos and investigated whether tetraploid blastomere aggregation could enhance the quality of tetraploid embryos. Three methods were investigated to produce tetraploid embryos: First, tetraploid embryos were produced using electro-fusion of two-cell stage parthenogenetic blastomere (FUTP). Second, somatic cell was injected into the mature oocyte and fused to produce tetraploid embryos. Third, oocytes were matured with Cytochalasin B (CB) for the late 22 h of in vitro maturation to inhibit the first polar body (PB1). Following that, non-PB1 oocytes were treated with CB for 4 h after parthenogenetic activation. There was no significant difference in the blastocyst development rate and tetraploid production rate of the embryos produced through the three methods. However, FUTP-derived blastocysts had a significantly lower percentage of apoptotic cells compared to other methods. The developmental competence of embryos, expression of trophectoderm cell marker genes, and distribution of YAP1 protein were investigated in tetraploid embryos produced using the FUTP method. The FUTP method most effectively prevented apoptosis during porcine tetraploid embryo formation. Tetraploid aggregation-derived blastocysts have a high proportion of trophectoderm with increased expression of the CDX2 mRNA and high YAP1 intensity. High-quality blastocysts derived from a tetraploid embryo aggregation can serve as suitable source material for testing the differentiation potential of pluripotent stem cells for blastocyst complementation in pigs. |
format | Online Article Text |
id | pubmed-10687364 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-106873642023-11-30 Tetraploid embryo aggregation produces high-quality blastocysts with an increased trophectoderm in pigs Lee, Joohyeong Cai, Lian Kim, Mirae Choi, Hyerin Oh, Dongjin Jawad, Ali Lee, Eunsong Hyun, Sang-Hwan Front Cell Dev Biol Cell and Developmental Biology Tetraploid complementation is an ideal method for demonstrating the differentiation potential of pluripotent stem cells. In this study, we selected the most efficient tetraploid production method for porcine embryos and investigated whether tetraploid blastomere aggregation could enhance the quality of tetraploid embryos. Three methods were investigated to produce tetraploid embryos: First, tetraploid embryos were produced using electro-fusion of two-cell stage parthenogenetic blastomere (FUTP). Second, somatic cell was injected into the mature oocyte and fused to produce tetraploid embryos. Third, oocytes were matured with Cytochalasin B (CB) for the late 22 h of in vitro maturation to inhibit the first polar body (PB1). Following that, non-PB1 oocytes were treated with CB for 4 h after parthenogenetic activation. There was no significant difference in the blastocyst development rate and tetraploid production rate of the embryos produced through the three methods. However, FUTP-derived blastocysts had a significantly lower percentage of apoptotic cells compared to other methods. The developmental competence of embryos, expression of trophectoderm cell marker genes, and distribution of YAP1 protein were investigated in tetraploid embryos produced using the FUTP method. The FUTP method most effectively prevented apoptosis during porcine tetraploid embryo formation. Tetraploid aggregation-derived blastocysts have a high proportion of trophectoderm with increased expression of the CDX2 mRNA and high YAP1 intensity. High-quality blastocysts derived from a tetraploid embryo aggregation can serve as suitable source material for testing the differentiation potential of pluripotent stem cells for blastocyst complementation in pigs. Frontiers Media S.A. 2023-11-16 /pmc/articles/PMC10687364/ /pubmed/38033873 http://dx.doi.org/10.3389/fcell.2023.1239448 Text en Copyright © 2023 Lee, Cai, Kim, Choi, Oh, Jawad, Lee and Hyun. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Cell and Developmental Biology Lee, Joohyeong Cai, Lian Kim, Mirae Choi, Hyerin Oh, Dongjin Jawad, Ali Lee, Eunsong Hyun, Sang-Hwan Tetraploid embryo aggregation produces high-quality blastocysts with an increased trophectoderm in pigs |
title | Tetraploid embryo aggregation produces high-quality blastocysts with an increased trophectoderm in pigs |
title_full | Tetraploid embryo aggregation produces high-quality blastocysts with an increased trophectoderm in pigs |
title_fullStr | Tetraploid embryo aggregation produces high-quality blastocysts with an increased trophectoderm in pigs |
title_full_unstemmed | Tetraploid embryo aggregation produces high-quality blastocysts with an increased trophectoderm in pigs |
title_short | Tetraploid embryo aggregation produces high-quality blastocysts with an increased trophectoderm in pigs |
title_sort | tetraploid embryo aggregation produces high-quality blastocysts with an increased trophectoderm in pigs |
topic | Cell and Developmental Biology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10687364/ https://www.ncbi.nlm.nih.gov/pubmed/38033873 http://dx.doi.org/10.3389/fcell.2023.1239448 |
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