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Molecular survey and phylogenetic analysis of Bartonella sp., Coxiella sp., and hemoplamas in pudu (Pudu puda) from Chile: first report of Bartonella henselae in a wild ungulate species
INTRODUCTION: Recent evidence shows a high diversity of infectious agents in wildlife that represent a threat to human, domestic, and wild animal health. In Chile, wild populations of the most common cervid species, pudu (Pudu puda), have been reported as hosts for novel pathogens such as Mycoplasma...
Autores principales: | , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2023
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10687462/ https://www.ncbi.nlm.nih.gov/pubmed/38033637 http://dx.doi.org/10.3389/fvets.2023.1161093 |
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author | Hidalgo-Hermoso, Ezequiel Sepúlveda-García, Paulina Cabello, Javier Celis, Sebastian Valencia, Carola Ortiz, Carolina Kemec, Ignacio Moreira-Arce, Dario Orsola, Miguel Canales, Nivia Garnham, Antonio Vera, Frank Muller, Ananda |
author_facet | Hidalgo-Hermoso, Ezequiel Sepúlveda-García, Paulina Cabello, Javier Celis, Sebastian Valencia, Carola Ortiz, Carolina Kemec, Ignacio Moreira-Arce, Dario Orsola, Miguel Canales, Nivia Garnham, Antonio Vera, Frank Muller, Ananda |
author_sort | Hidalgo-Hermoso, Ezequiel |
collection | PubMed |
description | INTRODUCTION: Recent evidence shows a high diversity of infectious agents in wildlife that represent a threat to human, domestic, and wild animal health. In Chile, wild populations of the most common cervid species, pudu (Pudu puda), have been reported as hosts for novel pathogens such as Mycoplasma ovis-like and a novel ecotype of Anaplasma phagocytophilum. A better understanding of the epidemiology of this group and other intracellular bacteria that might have cervids as hosts would enlighten their population relevance. This study aimed to determine the occurrence and genetic diversity of Bartonella spp., hemotropic mycoplasmas, and Coxiella burnetii in pudus from Chile. METHODS: The DNA was extracted from the blood samples of 69 wild free-ranging and 30 captive pudus from Chile. A combination of real-time (nouG gene for Bartonella and IS1111 element for C. burnetii) and conventional PCR (16S rRNA for hemotropic Mycoplasma spp. and rpoB, gltA, and ITS for Bartonella spp.) was used for pathogen screening and molecular characterization. RESULTS: DNA of Bartonella spp. was detected in 10.1% [95% CI (5.2–18.2%)] samples, hemotropic Mycoplasma spp. in 1.7% [95% CI (0.08–10.1%)], and C. burnetii in 1.0% [95% CI (0.05–6.3%)] samples. Two sequenced samples were identified as Mycoplasma ovis-like, and one free-ranging pudu was positive for C. burnetii. While one captive and two free-ranging pudus were positive for Bartonella henselae, one wild pudu was co-positive for B. henselae and Bartonella sp., similar to Bartonellae identified in ruminants. DISCUSSION: To the best of our knowledge, this is the first report of B. henselae in wild ungulate species, and C. burnetii and Bartonella spp. in wild ungulate species in South America. Further research will be necessary to evaluate the potential role of pudu as reservoirs of infection and identify the sources for disease transmission among humans and wild and domestic animals. |
format | Online Article Text |
id | pubmed-10687462 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-106874622023-11-30 Molecular survey and phylogenetic analysis of Bartonella sp., Coxiella sp., and hemoplamas in pudu (Pudu puda) from Chile: first report of Bartonella henselae in a wild ungulate species Hidalgo-Hermoso, Ezequiel Sepúlveda-García, Paulina Cabello, Javier Celis, Sebastian Valencia, Carola Ortiz, Carolina Kemec, Ignacio Moreira-Arce, Dario Orsola, Miguel Canales, Nivia Garnham, Antonio Vera, Frank Muller, Ananda Front Vet Sci Veterinary Science INTRODUCTION: Recent evidence shows a high diversity of infectious agents in wildlife that represent a threat to human, domestic, and wild animal health. In Chile, wild populations of the most common cervid species, pudu (Pudu puda), have been reported as hosts for novel pathogens such as Mycoplasma ovis-like and a novel ecotype of Anaplasma phagocytophilum. A better understanding of the epidemiology of this group and other intracellular bacteria that might have cervids as hosts would enlighten their population relevance. This study aimed to determine the occurrence and genetic diversity of Bartonella spp., hemotropic mycoplasmas, and Coxiella burnetii in pudus from Chile. METHODS: The DNA was extracted from the blood samples of 69 wild free-ranging and 30 captive pudus from Chile. A combination of real-time (nouG gene for Bartonella and IS1111 element for C. burnetii) and conventional PCR (16S rRNA for hemotropic Mycoplasma spp. and rpoB, gltA, and ITS for Bartonella spp.) was used for pathogen screening and molecular characterization. RESULTS: DNA of Bartonella spp. was detected in 10.1% [95% CI (5.2–18.2%)] samples, hemotropic Mycoplasma spp. in 1.7% [95% CI (0.08–10.1%)], and C. burnetii in 1.0% [95% CI (0.05–6.3%)] samples. Two sequenced samples were identified as Mycoplasma ovis-like, and one free-ranging pudu was positive for C. burnetii. While one captive and two free-ranging pudus were positive for Bartonella henselae, one wild pudu was co-positive for B. henselae and Bartonella sp., similar to Bartonellae identified in ruminants. DISCUSSION: To the best of our knowledge, this is the first report of B. henselae in wild ungulate species, and C. burnetii and Bartonella spp. in wild ungulate species in South America. Further research will be necessary to evaluate the potential role of pudu as reservoirs of infection and identify the sources for disease transmission among humans and wild and domestic animals. Frontiers Media S.A. 2023-11-16 /pmc/articles/PMC10687462/ /pubmed/38033637 http://dx.doi.org/10.3389/fvets.2023.1161093 Text en Copyright © 2023 Hidalgo-Hermoso, Sepúlveda-García, Cabello, Celis, Valencia, Ortiz, Kemec, Moreira-Arce, Orsola, Canales, Garnham, Vera and Muller. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Veterinary Science Hidalgo-Hermoso, Ezequiel Sepúlveda-García, Paulina Cabello, Javier Celis, Sebastian Valencia, Carola Ortiz, Carolina Kemec, Ignacio Moreira-Arce, Dario Orsola, Miguel Canales, Nivia Garnham, Antonio Vera, Frank Muller, Ananda Molecular survey and phylogenetic analysis of Bartonella sp., Coxiella sp., and hemoplamas in pudu (Pudu puda) from Chile: first report of Bartonella henselae in a wild ungulate species |
title | Molecular survey and phylogenetic analysis of Bartonella sp., Coxiella sp., and hemoplamas in pudu (Pudu puda) from Chile: first report of Bartonella henselae in a wild ungulate species |
title_full | Molecular survey and phylogenetic analysis of Bartonella sp., Coxiella sp., and hemoplamas in pudu (Pudu puda) from Chile: first report of Bartonella henselae in a wild ungulate species |
title_fullStr | Molecular survey and phylogenetic analysis of Bartonella sp., Coxiella sp., and hemoplamas in pudu (Pudu puda) from Chile: first report of Bartonella henselae in a wild ungulate species |
title_full_unstemmed | Molecular survey and phylogenetic analysis of Bartonella sp., Coxiella sp., and hemoplamas in pudu (Pudu puda) from Chile: first report of Bartonella henselae in a wild ungulate species |
title_short | Molecular survey and phylogenetic analysis of Bartonella sp., Coxiella sp., and hemoplamas in pudu (Pudu puda) from Chile: first report of Bartonella henselae in a wild ungulate species |
title_sort | molecular survey and phylogenetic analysis of bartonella sp., coxiella sp., and hemoplamas in pudu (pudu puda) from chile: first report of bartonella henselae in a wild ungulate species |
topic | Veterinary Science |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10687462/ https://www.ncbi.nlm.nih.gov/pubmed/38033637 http://dx.doi.org/10.3389/fvets.2023.1161093 |
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