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Cold stress induces differential gene expression of retained homeologs in Camelina sativa cv Suneson

Camelina sativa (L.) Crantz, a member of the Brassicaceae, has potential as a biofuel feedstock which is attributable to the production of fatty acids in its seeds, its fast growth cycle, and low input requirements. While a genome assembly is available for camelina, it was generated from short seque...

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Autores principales: Fang, Chao, Hamilton, John P., Vaillancourt, Brieanne, Wang, Yi-Wen, Wood, Joshua C., Deans, Natalie C., Scroggs, Taylor, Carlton, Lemor, Mailloux, Kathrine, Douches, David S., Nadakuduti, Satya Swathi, Jiang, Jiming, Buell, C. Robin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10687638/
https://www.ncbi.nlm.nih.gov/pubmed/38034564
http://dx.doi.org/10.3389/fpls.2023.1271625
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author Fang, Chao
Hamilton, John P.
Vaillancourt, Brieanne
Wang, Yi-Wen
Wood, Joshua C.
Deans, Natalie C.
Scroggs, Taylor
Carlton, Lemor
Mailloux, Kathrine
Douches, David S.
Nadakuduti, Satya Swathi
Jiang, Jiming
Buell, C. Robin
author_facet Fang, Chao
Hamilton, John P.
Vaillancourt, Brieanne
Wang, Yi-Wen
Wood, Joshua C.
Deans, Natalie C.
Scroggs, Taylor
Carlton, Lemor
Mailloux, Kathrine
Douches, David S.
Nadakuduti, Satya Swathi
Jiang, Jiming
Buell, C. Robin
author_sort Fang, Chao
collection PubMed
description Camelina sativa (L.) Crantz, a member of the Brassicaceae, has potential as a biofuel feedstock which is attributable to the production of fatty acids in its seeds, its fast growth cycle, and low input requirements. While a genome assembly is available for camelina, it was generated from short sequence reads and is thus highly fragmented in nature. Using long read sequences, we generated a chromosome-scale, highly contiguous genome assembly (644,491,969 bp) for the spring biotype cultivar ‘Suneson’ with an N50 contig length of 12,031,512 bp and a scaffold N50 length of 32,184,682 bp. Annotation of protein-coding genes revealed 91,877 genes that encode 133,355 gene models. We identified a total of 4,467 genes that were significantly up-regulated under cold stress which were enriched in gene ontology terms associated with “response to cold” and “response to abiotic stress”. Coexpression analyses revealed multiple coexpression modules that were enriched in genes differentially expressed following cold stress that had putative functions involved in stress adaptation, specifically within the plastid. With access to a highly contiguous genome assembly, comparative analyses with Arabidopsis thaliana revealed 23,625 A. thaliana genes syntenic with 45,453 Suneson genes. Of these, 24,960 Suneson genes were syntenic to 8,320 A. thaliana genes reflecting a 3 camelina homeolog to 1 Arabidopsis gene relationship and retention of all three homeologs. Some of the retained triplicated homeologs showed conserved gene expression patterns under control and cold-stressed conditions whereas other triplicated homeologs displayed diverged expression patterns revealing sub- and neo-functionalization of the homeologs at the transcription level. Access to the chromosome-scale assembly of Suneson will enable both basic and applied research efforts in the improvement of camelina as a sustainable biofuel feedstock.
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spelling pubmed-106876382023-11-30 Cold stress induces differential gene expression of retained homeologs in Camelina sativa cv Suneson Fang, Chao Hamilton, John P. Vaillancourt, Brieanne Wang, Yi-Wen Wood, Joshua C. Deans, Natalie C. Scroggs, Taylor Carlton, Lemor Mailloux, Kathrine Douches, David S. Nadakuduti, Satya Swathi Jiang, Jiming Buell, C. Robin Front Plant Sci Plant Science Camelina sativa (L.) Crantz, a member of the Brassicaceae, has potential as a biofuel feedstock which is attributable to the production of fatty acids in its seeds, its fast growth cycle, and low input requirements. While a genome assembly is available for camelina, it was generated from short sequence reads and is thus highly fragmented in nature. Using long read sequences, we generated a chromosome-scale, highly contiguous genome assembly (644,491,969 bp) for the spring biotype cultivar ‘Suneson’ with an N50 contig length of 12,031,512 bp and a scaffold N50 length of 32,184,682 bp. Annotation of protein-coding genes revealed 91,877 genes that encode 133,355 gene models. We identified a total of 4,467 genes that were significantly up-regulated under cold stress which were enriched in gene ontology terms associated with “response to cold” and “response to abiotic stress”. Coexpression analyses revealed multiple coexpression modules that were enriched in genes differentially expressed following cold stress that had putative functions involved in stress adaptation, specifically within the plastid. With access to a highly contiguous genome assembly, comparative analyses with Arabidopsis thaliana revealed 23,625 A. thaliana genes syntenic with 45,453 Suneson genes. Of these, 24,960 Suneson genes were syntenic to 8,320 A. thaliana genes reflecting a 3 camelina homeolog to 1 Arabidopsis gene relationship and retention of all three homeologs. Some of the retained triplicated homeologs showed conserved gene expression patterns under control and cold-stressed conditions whereas other triplicated homeologs displayed diverged expression patterns revealing sub- and neo-functionalization of the homeologs at the transcription level. Access to the chromosome-scale assembly of Suneson will enable both basic and applied research efforts in the improvement of camelina as a sustainable biofuel feedstock. Frontiers Media S.A. 2023-11-16 /pmc/articles/PMC10687638/ /pubmed/38034564 http://dx.doi.org/10.3389/fpls.2023.1271625 Text en Copyright © 2023 Fang, Hamilton, Vaillancourt, Wang, Wood, Deans, Scroggs, Carlton, Mailloux, Douches, Nadakuduti, Jiang and Buell https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Plant Science
Fang, Chao
Hamilton, John P.
Vaillancourt, Brieanne
Wang, Yi-Wen
Wood, Joshua C.
Deans, Natalie C.
Scroggs, Taylor
Carlton, Lemor
Mailloux, Kathrine
Douches, David S.
Nadakuduti, Satya Swathi
Jiang, Jiming
Buell, C. Robin
Cold stress induces differential gene expression of retained homeologs in Camelina sativa cv Suneson
title Cold stress induces differential gene expression of retained homeologs in Camelina sativa cv Suneson
title_full Cold stress induces differential gene expression of retained homeologs in Camelina sativa cv Suneson
title_fullStr Cold stress induces differential gene expression of retained homeologs in Camelina sativa cv Suneson
title_full_unstemmed Cold stress induces differential gene expression of retained homeologs in Camelina sativa cv Suneson
title_short Cold stress induces differential gene expression of retained homeologs in Camelina sativa cv Suneson
title_sort cold stress induces differential gene expression of retained homeologs in camelina sativa cv suneson
topic Plant Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10687638/
https://www.ncbi.nlm.nih.gov/pubmed/38034564
http://dx.doi.org/10.3389/fpls.2023.1271625
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