Cargando…

Emerging Time-Resolved X-Ray Diffraction Approaches for Protein Dynamics

Proteins guide the flows of information, energy, and matter that make life possible by accelerating transport and chemical reactions, by allosterically modulating these reactions, and by forming dynamic supramolecular assemblies. In these roles, conformational change underlies functional transitions...

Descripción completa

Detalles Bibliográficos
Autor principal: Hekstra, Doeke R.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10687665/
https://www.ncbi.nlm.nih.gov/pubmed/37159292
http://dx.doi.org/10.1146/annurev-biophys-111622-091155
_version_ 1785152020511981568
author Hekstra, Doeke R.
author_facet Hekstra, Doeke R.
author_sort Hekstra, Doeke R.
collection PubMed
description Proteins guide the flows of information, energy, and matter that make life possible by accelerating transport and chemical reactions, by allosterically modulating these reactions, and by forming dynamic supramolecular assemblies. In these roles, conformational change underlies functional transitions. Time-resolved X-ray diffraction methods characterize these transitions either by directly triggering sequences of functionally important motions or, more broadly, by capturing the motions of which proteins are capable. To date, most successful have been experiments in which conformational change is triggered in light-dependent proteins. In this review, I emphasize emerging techniques that probe the dynamic basis of function in proteins lacking natively light-dependent transitions and speculate about extensions and further possibilities. In addition, I review how the weaker and more distributed signals in these data push the limits of the capabilities of analytical methods. Taken together, these new methods are beginning to establish a powerful paradigm for the study of the physics of protein function.
format Online
Article
Text
id pubmed-10687665
institution National Center for Biotechnology Information
language English
publishDate 2023
record_format MEDLINE/PubMed
spelling pubmed-106876652023-11-30 Emerging Time-Resolved X-Ray Diffraction Approaches for Protein Dynamics Hekstra, Doeke R. Annu Rev Biophys Article Proteins guide the flows of information, energy, and matter that make life possible by accelerating transport and chemical reactions, by allosterically modulating these reactions, and by forming dynamic supramolecular assemblies. In these roles, conformational change underlies functional transitions. Time-resolved X-ray diffraction methods characterize these transitions either by directly triggering sequences of functionally important motions or, more broadly, by capturing the motions of which proteins are capable. To date, most successful have been experiments in which conformational change is triggered in light-dependent proteins. In this review, I emphasize emerging techniques that probe the dynamic basis of function in proteins lacking natively light-dependent transitions and speculate about extensions and further possibilities. In addition, I review how the weaker and more distributed signals in these data push the limits of the capabilities of analytical methods. Taken together, these new methods are beginning to establish a powerful paradigm for the study of the physics of protein function. 2023-05-09 /pmc/articles/PMC10687665/ /pubmed/37159292 http://dx.doi.org/10.1146/annurev-biophys-111622-091155 Text en https://creativecommons.org/licenses/by/4.0/This work is licensed under a Creative Commons Attribution 4.0 International License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited. See credit lines of images or other third-party material in this article for license information.
spellingShingle Article
Hekstra, Doeke R.
Emerging Time-Resolved X-Ray Diffraction Approaches for Protein Dynamics
title Emerging Time-Resolved X-Ray Diffraction Approaches for Protein Dynamics
title_full Emerging Time-Resolved X-Ray Diffraction Approaches for Protein Dynamics
title_fullStr Emerging Time-Resolved X-Ray Diffraction Approaches for Protein Dynamics
title_full_unstemmed Emerging Time-Resolved X-Ray Diffraction Approaches for Protein Dynamics
title_short Emerging Time-Resolved X-Ray Diffraction Approaches for Protein Dynamics
title_sort emerging time-resolved x-ray diffraction approaches for protein dynamics
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10687665/
https://www.ncbi.nlm.nih.gov/pubmed/37159292
http://dx.doi.org/10.1146/annurev-biophys-111622-091155
work_keys_str_mv AT hekstradoeker emergingtimeresolvedxraydiffractionapproachesforproteindynamics