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Quantitative tools in microbial and chemical risk assessment

The EU‐FORA programme ‘Quantitative tools in microbial and chemical risk assessment’ was dedicated to training on predictive microbiology fundamentals, implementation of different modelling strategies, design of experiments and software tools such as MATLAB, GInaFiT and DMFit. The fellow performed M...

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Detalles Bibliográficos
Autores principales: Stratev, Deyan, Valdramidis, Vasilis P
Formato: Online Artículo Texto
Lenguaje:English
Publicado: John Wiley and Sons Inc. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10687750/
https://www.ncbi.nlm.nih.gov/pubmed/38047128
http://dx.doi.org/10.2903/j.efsa.2023.e211016
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author Stratev, Deyan
Valdramidis, Vasilis P
author_facet Stratev, Deyan
Valdramidis, Vasilis P
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description The EU‐FORA programme ‘Quantitative tools in microbial and chemical risk assessment’ was dedicated to training on predictive microbiology fundamentals, implementation of different modelling strategies, design of experiments and software tools such as MATLAB, GInaFiT and DMFit. The fellow performed MATLAB training on maximum specific growth rate (μ(max)) determination according to the Ratkowsky model. GInaFiT training on different models for bacterial inactivation and DMFit training on growth parameters of Vibrio parahaemolyticus were also carried out. Optical density measurements of V. parahaemolyticus bacterial cultures were performed. The obtained kinetics of optical density measurements were used to estimate μ(max). Hereafter, Minimum inhibitory concentrations and non‐inhibitory concentrations of aminoglycoside antibiotics were estimated based on the quantification of the fractional areas of the optical density vs time. It can be concluded that the results of the quantitative characterisation of V. parahaemolyticus are reliable and can be used for exposure assessments. Also, the turbidimetric assay can be applied for successful estimation of minimum inhibitory concentrations and non‐inhibitory concentrations.
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spelling pubmed-106877502023-12-01 Quantitative tools in microbial and chemical risk assessment Stratev, Deyan Valdramidis, Vasilis P EFSA J Eu‐fora Series 6 The EU‐FORA programme ‘Quantitative tools in microbial and chemical risk assessment’ was dedicated to training on predictive microbiology fundamentals, implementation of different modelling strategies, design of experiments and software tools such as MATLAB, GInaFiT and DMFit. The fellow performed MATLAB training on maximum specific growth rate (μ(max)) determination according to the Ratkowsky model. GInaFiT training on different models for bacterial inactivation and DMFit training on growth parameters of Vibrio parahaemolyticus were also carried out. Optical density measurements of V. parahaemolyticus bacterial cultures were performed. The obtained kinetics of optical density measurements were used to estimate μ(max). Hereafter, Minimum inhibitory concentrations and non‐inhibitory concentrations of aminoglycoside antibiotics were estimated based on the quantification of the fractional areas of the optical density vs time. It can be concluded that the results of the quantitative characterisation of V. parahaemolyticus are reliable and can be used for exposure assessments. Also, the turbidimetric assay can be applied for successful estimation of minimum inhibitory concentrations and non‐inhibitory concentrations. John Wiley and Sons Inc. 2023-11-30 /pmc/articles/PMC10687750/ /pubmed/38047128 http://dx.doi.org/10.2903/j.efsa.2023.e211016 Text en © 2023 European Food Safety Authority. EFSA Journal published by Wiley‐VCH GmbH on behalf of European Food Safety Authority. https://creativecommons.org/licenses/by-nd/4.0/This is an open access article under the terms of the http://creativecommons.org/licenses/by-nd/4.0/ (https://creativecommons.org/licenses/by-nd/4.0/) License, which permits use and distribution in any medium, provided the original work is properly cited and no modifications or adaptations are made.
spellingShingle Eu‐fora Series 6
Stratev, Deyan
Valdramidis, Vasilis P
Quantitative tools in microbial and chemical risk assessment
title Quantitative tools in microbial and chemical risk assessment
title_full Quantitative tools in microbial and chemical risk assessment
title_fullStr Quantitative tools in microbial and chemical risk assessment
title_full_unstemmed Quantitative tools in microbial and chemical risk assessment
title_short Quantitative tools in microbial and chemical risk assessment
title_sort quantitative tools in microbial and chemical risk assessment
topic Eu‐fora Series 6
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10687750/
https://www.ncbi.nlm.nih.gov/pubmed/38047128
http://dx.doi.org/10.2903/j.efsa.2023.e211016
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