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Lipid profiling reveals Leymus Chinensis root insensitivity to Ca limitation

BACKGROUND: Leymus chinensis (L. chinensis) is a perennial native forage grass widely distributed in the steppe of Inner Mongolia as the dominant species. Calcium (Ca) is an essential mineral element important for plant adaptation to the growth environment. Ca limitation was previously shown to stro...

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Autores principales: Nan, Yang, Dong, Yanbing, Zhang, Lili, Zhang, Lijuan, Qi, Zhi, Luan, Haiye, Yang, Ju
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10687902/
https://www.ncbi.nlm.nih.gov/pubmed/38031030
http://dx.doi.org/10.1186/s12870-023-04627-8
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author Nan, Yang
Dong, Yanbing
Zhang, Lili
Zhang, Lijuan
Qi, Zhi
Luan, Haiye
Yang, Ju
author_facet Nan, Yang
Dong, Yanbing
Zhang, Lili
Zhang, Lijuan
Qi, Zhi
Luan, Haiye
Yang, Ju
author_sort Nan, Yang
collection PubMed
description BACKGROUND: Leymus chinensis (L. chinensis) is a perennial native forage grass widely distributed in the steppe of Inner Mongolia as the dominant species. Calcium (Ca) is an essential mineral element important for plant adaptation to the growth environment. Ca limitation was previously shown to strongly inhibit Arabidopsis (Arabidopsis thaliana) seedling growth and disrupt plasma membrane stability and selectivity, increasing fluid-phase-based endocytosis and contents of all major membrane lipids. RESULTS: In this study, we investigated the significance of Ca for L. chinensis growth and membrane stability relative to Arabidopsis. Our results showed that Ca limitation did not affect L. chinensis seedling growth and endocytosis in roots. Moreover, the plasma membrane maintained high selectivity. The lipid phosphatidylcholine (PC): phosphatidylethanolamine (PE) ratio, an indicator of the membrane stability, was five times higher in L. chinensis than in Arabidopsis. Furthermore, in L. chinensis, Ca limitation did not affect the content of any major lipid types, decreased malondialdehyde (MDA) content, and increased superoxide dismutase (SOD) activity, showing an opposite pattern to that in Arabidopsis. L. chinensis roots accumulated higher contents of PC, phosphatidylinositol (PI), monogalactosyldiacylglycerol (MGDG), phosphatidylglycerol (PG), cardiolipin (CL), digalactosyldiacylglycerol (DGDG), and lysophosphatidylcholine (LPC) but less phosphatidylethanolamine (PE), diacylglycerol (DAG), triacylglycerolv (TAG), phosphatidylserine (PS), lysobisphosphatidic acids (LPAs), lysophosphatidylethanolamine (LPE), and lysophosphatidylserine (LPS) than Arabidopsis roots. Moreover, we detected 31 and 66 unique lipids in L. chinensis and Arabidopsis, respectively. CONCLUSIONS: This study revealed that L. chinensis roots have unique membrane lipid composition that was not sensitive to Ca limitation, which might contribute to the wider natural distribution of this species. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12870-023-04627-8.
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spelling pubmed-106879022023-11-30 Lipid profiling reveals Leymus Chinensis root insensitivity to Ca limitation Nan, Yang Dong, Yanbing Zhang, Lili Zhang, Lijuan Qi, Zhi Luan, Haiye Yang, Ju BMC Plant Biol Research BACKGROUND: Leymus chinensis (L. chinensis) is a perennial native forage grass widely distributed in the steppe of Inner Mongolia as the dominant species. Calcium (Ca) is an essential mineral element important for plant adaptation to the growth environment. Ca limitation was previously shown to strongly inhibit Arabidopsis (Arabidopsis thaliana) seedling growth and disrupt plasma membrane stability and selectivity, increasing fluid-phase-based endocytosis and contents of all major membrane lipids. RESULTS: In this study, we investigated the significance of Ca for L. chinensis growth and membrane stability relative to Arabidopsis. Our results showed that Ca limitation did not affect L. chinensis seedling growth and endocytosis in roots. Moreover, the plasma membrane maintained high selectivity. The lipid phosphatidylcholine (PC): phosphatidylethanolamine (PE) ratio, an indicator of the membrane stability, was five times higher in L. chinensis than in Arabidopsis. Furthermore, in L. chinensis, Ca limitation did not affect the content of any major lipid types, decreased malondialdehyde (MDA) content, and increased superoxide dismutase (SOD) activity, showing an opposite pattern to that in Arabidopsis. L. chinensis roots accumulated higher contents of PC, phosphatidylinositol (PI), monogalactosyldiacylglycerol (MGDG), phosphatidylglycerol (PG), cardiolipin (CL), digalactosyldiacylglycerol (DGDG), and lysophosphatidylcholine (LPC) but less phosphatidylethanolamine (PE), diacylglycerol (DAG), triacylglycerolv (TAG), phosphatidylserine (PS), lysobisphosphatidic acids (LPAs), lysophosphatidylethanolamine (LPE), and lysophosphatidylserine (LPS) than Arabidopsis roots. Moreover, we detected 31 and 66 unique lipids in L. chinensis and Arabidopsis, respectively. CONCLUSIONS: This study revealed that L. chinensis roots have unique membrane lipid composition that was not sensitive to Ca limitation, which might contribute to the wider natural distribution of this species. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12870-023-04627-8. BioMed Central 2023-11-30 /pmc/articles/PMC10687902/ /pubmed/38031030 http://dx.doi.org/10.1186/s12870-023-04627-8 Text en © The Author(s) 2023 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Research
Nan, Yang
Dong, Yanbing
Zhang, Lili
Zhang, Lijuan
Qi, Zhi
Luan, Haiye
Yang, Ju
Lipid profiling reveals Leymus Chinensis root insensitivity to Ca limitation
title Lipid profiling reveals Leymus Chinensis root insensitivity to Ca limitation
title_full Lipid profiling reveals Leymus Chinensis root insensitivity to Ca limitation
title_fullStr Lipid profiling reveals Leymus Chinensis root insensitivity to Ca limitation
title_full_unstemmed Lipid profiling reveals Leymus Chinensis root insensitivity to Ca limitation
title_short Lipid profiling reveals Leymus Chinensis root insensitivity to Ca limitation
title_sort lipid profiling reveals leymus chinensis root insensitivity to ca limitation
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10687902/
https://www.ncbi.nlm.nih.gov/pubmed/38031030
http://dx.doi.org/10.1186/s12870-023-04627-8
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