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Zein-Induced Polyelectrolyte Complexes for Encapsulating Triterpenoid Phytochemicals

[Image: see text] The hydrophobicity and aggregation of zein, a biopolymer, limit its application as an effective drug delivery carrier. Here, we developed a zein-induced polyelectrolyte (ZiP) complex and investigated its efficiency in delivering 1% hydrolyzed ginseng saponin, a compound K-rich frac...

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Autores principales: Kim, Yong-Jin, Lee, Eun-Soo, Choi, Joonho, Park, SeungHan, Chae, Byungguen, Kim, Eunmi
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Chemical Society 2023
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10687950/
https://www.ncbi.nlm.nih.gov/pubmed/38046302
http://dx.doi.org/10.1021/acsomega.3c05157
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author Kim, Yong-Jin
Lee, Eun-Soo
Choi, Joonho
Park, SeungHan
Chae, Byungguen
Kim, Eunmi
author_facet Kim, Yong-Jin
Lee, Eun-Soo
Choi, Joonho
Park, SeungHan
Chae, Byungguen
Kim, Eunmi
author_sort Kim, Yong-Jin
collection PubMed
description [Image: see text] The hydrophobicity and aggregation of zein, a biopolymer, limit its application as an effective drug delivery carrier. Here, we developed a zein-induced polyelectrolyte (ZiP) complex and investigated its efficiency in delivering 1% hydrolyzed ginseng saponin, a compound K-rich fraction derived from the root of Panax ginseng. The ZiP complex was formulated by incorporating the self-assembled amphiphilic prolamin zein into the aqueous phase. The physical properties, encapsulation efficiency, and stability of the encapsulation system at room temperature (25 °C) and 45 °C were assessed. The effects of different ratios of zein, pullulan, and pectin on the formation of the ZiP complex, the encapsulation stability, and the cellular efficacy of ZiP complexes were also assessed. The ZiP complex was surface-modified with hydrophilic pullulan and pectin polysaccharides in a mass ratio of 1:2:0.2 through electrostatic interactions. The primary hydrophilic modification of the ZiP complex was formed by the adsorption of pullulan, which enhanced the encapsulation stability. The outermost hydrophilic layer comprised the gelling polysaccharide pectin, which further improved the stability of the macro-sized oil-encapsulated complex, reaching sizes over 50 μm. The size of the ZiP complex increased when the concentration of pectin or the total content of the ZiP complex increased to 2:4:0.2. Compound K was successfully encapsulated with a particle size of 294.8 nm and an encapsulation efficiency of 99.6%. The ZiP complex demonstrated stability at high temperatures and long-term stability of the encapsulated saponin over 24 weeks. These results revealed the potency of ZiP complexes that enhance the in vivo absorption of phytochemicals as effective drug delivery carriers that can overcome the limitations in industrial formulation development as a delivery system.
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spelling pubmed-106879502023-12-01 Zein-Induced Polyelectrolyte Complexes for Encapsulating Triterpenoid Phytochemicals Kim, Yong-Jin Lee, Eun-Soo Choi, Joonho Park, SeungHan Chae, Byungguen Kim, Eunmi ACS Omega [Image: see text] The hydrophobicity and aggregation of zein, a biopolymer, limit its application as an effective drug delivery carrier. Here, we developed a zein-induced polyelectrolyte (ZiP) complex and investigated its efficiency in delivering 1% hydrolyzed ginseng saponin, a compound K-rich fraction derived from the root of Panax ginseng. The ZiP complex was formulated by incorporating the self-assembled amphiphilic prolamin zein into the aqueous phase. The physical properties, encapsulation efficiency, and stability of the encapsulation system at room temperature (25 °C) and 45 °C were assessed. The effects of different ratios of zein, pullulan, and pectin on the formation of the ZiP complex, the encapsulation stability, and the cellular efficacy of ZiP complexes were also assessed. The ZiP complex was surface-modified with hydrophilic pullulan and pectin polysaccharides in a mass ratio of 1:2:0.2 through electrostatic interactions. The primary hydrophilic modification of the ZiP complex was formed by the adsorption of pullulan, which enhanced the encapsulation stability. The outermost hydrophilic layer comprised the gelling polysaccharide pectin, which further improved the stability of the macro-sized oil-encapsulated complex, reaching sizes over 50 μm. The size of the ZiP complex increased when the concentration of pectin or the total content of the ZiP complex increased to 2:4:0.2. Compound K was successfully encapsulated with a particle size of 294.8 nm and an encapsulation efficiency of 99.6%. The ZiP complex demonstrated stability at high temperatures and long-term stability of the encapsulated saponin over 24 weeks. These results revealed the potency of ZiP complexes that enhance the in vivo absorption of phytochemicals as effective drug delivery carriers that can overcome the limitations in industrial formulation development as a delivery system. American Chemical Society 2023-11-13 /pmc/articles/PMC10687950/ /pubmed/38046302 http://dx.doi.org/10.1021/acsomega.3c05157 Text en © 2023 The Authors. Published by American Chemical Society https://creativecommons.org/licenses/by-nc-nd/4.0/Permits non-commercial access and re-use, provided that author attribution and integrity are maintained; but does not permit creation of adaptations or other derivative works (https://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Kim, Yong-Jin
Lee, Eun-Soo
Choi, Joonho
Park, SeungHan
Chae, Byungguen
Kim, Eunmi
Zein-Induced Polyelectrolyte Complexes for Encapsulating Triterpenoid Phytochemicals
title Zein-Induced Polyelectrolyte Complexes for Encapsulating Triterpenoid Phytochemicals
title_full Zein-Induced Polyelectrolyte Complexes for Encapsulating Triterpenoid Phytochemicals
title_fullStr Zein-Induced Polyelectrolyte Complexes for Encapsulating Triterpenoid Phytochemicals
title_full_unstemmed Zein-Induced Polyelectrolyte Complexes for Encapsulating Triterpenoid Phytochemicals
title_short Zein-Induced Polyelectrolyte Complexes for Encapsulating Triterpenoid Phytochemicals
title_sort zein-induced polyelectrolyte complexes for encapsulating triterpenoid phytochemicals
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10687950/
https://www.ncbi.nlm.nih.gov/pubmed/38046302
http://dx.doi.org/10.1021/acsomega.3c05157
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