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Changes of gut microbiota and short chain fatty acids in patients with Peutz–Jeghers syndrome

Peutz–Jeghers Syndromeis a rare autosomal dominant genetic disease characterized by gastrointestinal hamartomatous polyps and skin and mucous membrane pigmentation. The pathogenesis of PJS remains unclear; however, it may be associated with mutations in the STK11 gene, and there is currently no effe...

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Autores principales: Zhou, An, Tang, Bo, Xie, Yuhong, Li, Shengpeng, Xiao, Xu, Wu, Lingyi, Tu, Dianji, Wang, Sumin, Feng, Yunxuan, Feng, Xiaojie, Lai, Yi, Ning, Shoubin, Yang, Shiming
Formato: Online Artículo Texto
Lenguaje:English
Publicado: BioMed Central 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10688050/
https://www.ncbi.nlm.nih.gov/pubmed/38036954
http://dx.doi.org/10.1186/s12866-023-03132-0
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author Zhou, An
Tang, Bo
Xie, Yuhong
Li, Shengpeng
Xiao, Xu
Wu, Lingyi
Tu, Dianji
Wang, Sumin
Feng, Yunxuan
Feng, Xiaojie
Lai, Yi
Ning, Shoubin
Yang, Shiming
author_facet Zhou, An
Tang, Bo
Xie, Yuhong
Li, Shengpeng
Xiao, Xu
Wu, Lingyi
Tu, Dianji
Wang, Sumin
Feng, Yunxuan
Feng, Xiaojie
Lai, Yi
Ning, Shoubin
Yang, Shiming
author_sort Zhou, An
collection PubMed
description Peutz–Jeghers Syndromeis a rare autosomal dominant genetic disease characterized by gastrointestinal hamartomatous polyps and skin and mucous membrane pigmentation. The pathogenesis of PJS remains unclear; however, it may be associated with mutations in the STK11 gene, and there is currently no effective treatment available. The gut microbiota plays an important role in maintaining intestinal homeostasis in the human body, and an increasing number of studies have reported a relationship between gut microbiota and human health and disease. However, relatively few studies have been conducted on the gut microbiota characteristics of patients with PJS. In this study, we analyzed the characteristics of the gut microbiota of 79 patients with PJS using 16 S sequencing and measured the levels of short-chain fatty acids in the intestines. The results showed dysbiosis in the gut microbiota of patients with PJS, and decreased synthesis of short-chain fatty acids. Bacteroides was positively correlated with maximum polyp length, while Agathobacter was negatively correlated with age of onset. In addition, acetic acid, propionic acid, and butyric acid were positively correlated with the age of onset but negatively correlated with the number of polyps. Furthermore, the butyric acid level was negatively correlated with the frequency of endoscopic surgeries. In contrast, we compared the gut microbiota of STK11-positive and STK11-negative patients with PJS for the first time, but 16 S sequencing analysis revealed no significant differences. Finally, we established a random forest prediction model based on the gut microbiota characteristics of patients to provide a basis for the targeted diagnosis and treatment of PJS in the future. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12866-023-03132-0.
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spelling pubmed-106880502023-11-30 Changes of gut microbiota and short chain fatty acids in patients with Peutz–Jeghers syndrome Zhou, An Tang, Bo Xie, Yuhong Li, Shengpeng Xiao, Xu Wu, Lingyi Tu, Dianji Wang, Sumin Feng, Yunxuan Feng, Xiaojie Lai, Yi Ning, Shoubin Yang, Shiming BMC Microbiol Research Peutz–Jeghers Syndromeis a rare autosomal dominant genetic disease characterized by gastrointestinal hamartomatous polyps and skin and mucous membrane pigmentation. The pathogenesis of PJS remains unclear; however, it may be associated with mutations in the STK11 gene, and there is currently no effective treatment available. The gut microbiota plays an important role in maintaining intestinal homeostasis in the human body, and an increasing number of studies have reported a relationship between gut microbiota and human health and disease. However, relatively few studies have been conducted on the gut microbiota characteristics of patients with PJS. In this study, we analyzed the characteristics of the gut microbiota of 79 patients with PJS using 16 S sequencing and measured the levels of short-chain fatty acids in the intestines. The results showed dysbiosis in the gut microbiota of patients with PJS, and decreased synthesis of short-chain fatty acids. Bacteroides was positively correlated with maximum polyp length, while Agathobacter was negatively correlated with age of onset. In addition, acetic acid, propionic acid, and butyric acid were positively correlated with the age of onset but negatively correlated with the number of polyps. Furthermore, the butyric acid level was negatively correlated with the frequency of endoscopic surgeries. In contrast, we compared the gut microbiota of STK11-positive and STK11-negative patients with PJS for the first time, but 16 S sequencing analysis revealed no significant differences. Finally, we established a random forest prediction model based on the gut microbiota characteristics of patients to provide a basis for the targeted diagnosis and treatment of PJS in the future. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1186/s12866-023-03132-0. BioMed Central 2023-11-30 /pmc/articles/PMC10688050/ /pubmed/38036954 http://dx.doi.org/10.1186/s12866-023-03132-0 Text en © The Author(s) 2023 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/ (https://creativecommons.org/publicdomain/zero/1.0/) ) applies to the data made available in this article, unless otherwise stated in a credit line to the data.
spellingShingle Research
Zhou, An
Tang, Bo
Xie, Yuhong
Li, Shengpeng
Xiao, Xu
Wu, Lingyi
Tu, Dianji
Wang, Sumin
Feng, Yunxuan
Feng, Xiaojie
Lai, Yi
Ning, Shoubin
Yang, Shiming
Changes of gut microbiota and short chain fatty acids in patients with Peutz–Jeghers syndrome
title Changes of gut microbiota and short chain fatty acids in patients with Peutz–Jeghers syndrome
title_full Changes of gut microbiota and short chain fatty acids in patients with Peutz–Jeghers syndrome
title_fullStr Changes of gut microbiota and short chain fatty acids in patients with Peutz–Jeghers syndrome
title_full_unstemmed Changes of gut microbiota and short chain fatty acids in patients with Peutz–Jeghers syndrome
title_short Changes of gut microbiota and short chain fatty acids in patients with Peutz–Jeghers syndrome
title_sort changes of gut microbiota and short chain fatty acids in patients with peutz–jeghers syndrome
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10688050/
https://www.ncbi.nlm.nih.gov/pubmed/38036954
http://dx.doi.org/10.1186/s12866-023-03132-0
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