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Biomimetic nanoplatform with anti-inflammation and neuroprotective effects for repairing spinal cord injury in mice

Regeneration in the therapeutics of spinal cord injury (SCI) remains a challenge caused by the hyperinflammation microenvironment. Nanomaterials-based treatment strategies for diseases with excellent therapeutic efficacy are actively pursued. Here, we develop biodegradable poly (lactic-co-glycolic a...

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Detalles Bibliográficos
Autores principales: Yin, Xuechen, Lin, Sen, Xiong, Ying, Zhang, Peng, Mei, Xifan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10689280/
https://www.ncbi.nlm.nih.gov/pubmed/38046275
http://dx.doi.org/10.1016/j.mtbio.2023.100836
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author Yin, Xuechen
Lin, Sen
Xiong, Ying
Zhang, Peng
Mei, Xifan
author_facet Yin, Xuechen
Lin, Sen
Xiong, Ying
Zhang, Peng
Mei, Xifan
author_sort Yin, Xuechen
collection PubMed
description Regeneration in the therapeutics of spinal cord injury (SCI) remains a challenge caused by the hyperinflammation microenvironment. Nanomaterials-based treatment strategies for diseases with excellent therapeutic efficacy are actively pursued. Here, we develop biodegradable poly (lactic-co-glycolic acid) nanoparticles (PLGA) obtained by loading celastrol (pCel) for SCI thrapy. Cel, as an antioxidant drug, facilitated reactive oxygen species (ROS) scavenging, and decreased the generation of pro-inflammatory cytokines. To facilitate its administration, pCel is formulated into microspheres by oil-in-water (O/W) emulsion/solvent evaporation technique. The constructed pCel can induced polarization of macrophages and obviously improved lipopolysaccharide (LPS) and interferon-γ (IFN-γ)-induced mitochondrial dysfunction, and increased neurite length in PC12 cells and primary neurons. In vivo experiments revealed that pCel regulated the phenotypic polarization of macrophages, prevented the release of pro-inflammatory cytokines, promoted myelin regeneration and inhibited scar tissue formation, and further improve motor function. These findings indicated that the neuroprotective effect of this artificial biodegradable nanoplatform is benefit for the therapy of SCI. This research opens an exciting perspective for the application of SCI treatment and supports the clinical significance of pCel.
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spelling pubmed-106892802023-12-02 Biomimetic nanoplatform with anti-inflammation and neuroprotective effects for repairing spinal cord injury in mice Yin, Xuechen Lin, Sen Xiong, Ying Zhang, Peng Mei, Xifan Mater Today Bio Full Length Article Regeneration in the therapeutics of spinal cord injury (SCI) remains a challenge caused by the hyperinflammation microenvironment. Nanomaterials-based treatment strategies for diseases with excellent therapeutic efficacy are actively pursued. Here, we develop biodegradable poly (lactic-co-glycolic acid) nanoparticles (PLGA) obtained by loading celastrol (pCel) for SCI thrapy. Cel, as an antioxidant drug, facilitated reactive oxygen species (ROS) scavenging, and decreased the generation of pro-inflammatory cytokines. To facilitate its administration, pCel is formulated into microspheres by oil-in-water (O/W) emulsion/solvent evaporation technique. The constructed pCel can induced polarization of macrophages and obviously improved lipopolysaccharide (LPS) and interferon-γ (IFN-γ)-induced mitochondrial dysfunction, and increased neurite length in PC12 cells and primary neurons. In vivo experiments revealed that pCel regulated the phenotypic polarization of macrophages, prevented the release of pro-inflammatory cytokines, promoted myelin regeneration and inhibited scar tissue formation, and further improve motor function. These findings indicated that the neuroprotective effect of this artificial biodegradable nanoplatform is benefit for the therapy of SCI. This research opens an exciting perspective for the application of SCI treatment and supports the clinical significance of pCel. Elsevier 2023-11-10 /pmc/articles/PMC10689280/ /pubmed/38046275 http://dx.doi.org/10.1016/j.mtbio.2023.100836 Text en © 2023 The Author(s) https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Full Length Article
Yin, Xuechen
Lin, Sen
Xiong, Ying
Zhang, Peng
Mei, Xifan
Biomimetic nanoplatform with anti-inflammation and neuroprotective effects for repairing spinal cord injury in mice
title Biomimetic nanoplatform with anti-inflammation and neuroprotective effects for repairing spinal cord injury in mice
title_full Biomimetic nanoplatform with anti-inflammation and neuroprotective effects for repairing spinal cord injury in mice
title_fullStr Biomimetic nanoplatform with anti-inflammation and neuroprotective effects for repairing spinal cord injury in mice
title_full_unstemmed Biomimetic nanoplatform with anti-inflammation and neuroprotective effects for repairing spinal cord injury in mice
title_short Biomimetic nanoplatform with anti-inflammation and neuroprotective effects for repairing spinal cord injury in mice
title_sort biomimetic nanoplatform with anti-inflammation and neuroprotective effects for repairing spinal cord injury in mice
topic Full Length Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10689280/
https://www.ncbi.nlm.nih.gov/pubmed/38046275
http://dx.doi.org/10.1016/j.mtbio.2023.100836
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