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Methods for studying mammalian aquaporin biology

Aquaporins (AQPs), transmembrane water-conducting channels, have earned a great deal of scrutiny for their critical physiological roles in healthy and disease cell states, especially in the biomedical field. Numerous methods have been implemented to elucidate the involvement of AQP-mediated water tr...

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Detalles Bibliográficos
Autores principales: Banerjee, Shohini, Smith, Ian M, Hengen, Autumn C, Stroka, Kimberly M
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10689382/
https://www.ncbi.nlm.nih.gov/pubmed/38046463
http://dx.doi.org/10.1093/biomethods/bpad031
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author Banerjee, Shohini
Smith, Ian M
Hengen, Autumn C
Stroka, Kimberly M
author_facet Banerjee, Shohini
Smith, Ian M
Hengen, Autumn C
Stroka, Kimberly M
author_sort Banerjee, Shohini
collection PubMed
description Aquaporins (AQPs), transmembrane water-conducting channels, have earned a great deal of scrutiny for their critical physiological roles in healthy and disease cell states, especially in the biomedical field. Numerous methods have been implemented to elucidate the involvement of AQP-mediated water transport and downstream signaling activation in eliciting whole cell, tissue, and organ functional responses. To modulate these responses, other methods have been employed to investigate AQP druggability. This review discusses standard in vitro, in vivo, and in silico methods for studying AQPs, especially for biomedical and mammalian cell biology applications. We also propose some new techniques and approaches for future AQP research to address current gaps in methodology.
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spelling pubmed-106893822023-12-02 Methods for studying mammalian aquaporin biology Banerjee, Shohini Smith, Ian M Hengen, Autumn C Stroka, Kimberly M Biol Methods Protoc Review Aquaporins (AQPs), transmembrane water-conducting channels, have earned a great deal of scrutiny for their critical physiological roles in healthy and disease cell states, especially in the biomedical field. Numerous methods have been implemented to elucidate the involvement of AQP-mediated water transport and downstream signaling activation in eliciting whole cell, tissue, and organ functional responses. To modulate these responses, other methods have been employed to investigate AQP druggability. This review discusses standard in vitro, in vivo, and in silico methods for studying AQPs, especially for biomedical and mammalian cell biology applications. We also propose some new techniques and approaches for future AQP research to address current gaps in methodology. Oxford University Press 2023-11-11 /pmc/articles/PMC10689382/ /pubmed/38046463 http://dx.doi.org/10.1093/biomethods/bpad031 Text en © The Author(s) 2023. Published by Oxford University Press. https://creativecommons.org/licenses/by-nc/4.0/This is an Open Access article distributed under the terms of the Creative Commons Attribution-NonCommercial License (https://creativecommons.org/licenses/by-nc/4.0/), which permits non-commercial re-use, distribution, and reproduction in any medium, provided the original work is properly cited. For commercial re-use, please contact journals.permissions@oup.com
spellingShingle Review
Banerjee, Shohini
Smith, Ian M
Hengen, Autumn C
Stroka, Kimberly M
Methods for studying mammalian aquaporin biology
title Methods for studying mammalian aquaporin biology
title_full Methods for studying mammalian aquaporin biology
title_fullStr Methods for studying mammalian aquaporin biology
title_full_unstemmed Methods for studying mammalian aquaporin biology
title_short Methods for studying mammalian aquaporin biology
title_sort methods for studying mammalian aquaporin biology
topic Review
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10689382/
https://www.ncbi.nlm.nih.gov/pubmed/38046463
http://dx.doi.org/10.1093/biomethods/bpad031
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