Lysobacter changpingensis sp. nov., a novel species of the genus Lysobacter isolated from a rhizosphere soil of strawberry in China

In the present work, we characterized in detail strain CM-3-T8(T), which was isolated from the rhizosphere soil of strawberries in Beijing, China, in order to elucidate its taxonomic position. Cells of strain CM-3-T8(T) were Gram-negative, non-spore-forming, aerobic, short rod. Growth occurred at 25–37 °C, pH 5.0–10.0, and in the presence of 0–8% (w/v) NaCl. Phylogenetic analysis based on 16S rRNA gene sequences revealed that strain CM-3-T8(T) formed a stable clade with Lysobacter soli DCY21(T) and Lysobacter panacisoli CJ29(T), with the 16S rRNA gene sequence similarities of 98.91% and 98.50%. The average nucleotide identity and digital DNA-DNA hybridization values between strain SG-8( T) and the two reference type strains listed above were 76.3%, 79.6%, and 34.3%, 27%, respectively. The DNA G + C content was 68.4% (mol/mol). The major cellular fatty acids were comprised of C15:0 iso (36.15%), C17:0 iso (8.40%), and C11:0 iso 3OH (8.28%). The major quinone system was ubiquinone Q-8. The major polar lipids were phosphatidylethanolamine (PE), phosphatidylethanolamine (PME), diphosphatidylglycerol (DPG), and aminophospholipid (APL). On the basis of phenotypic, genotypic, and phylogenetic evidence, strain CM-3-T8(T) (= ACCC 61714( T) = JCM 34576( T)) represents a new species within the genus Lysobacter, for which the name Lysobacter changpingensis sp. nov. is proposed. SUPPLEMENTARY INFORMATION: The online version contains supplementary material available at 10.1007/s12223-023-01058-8.