Cargando…

Chromosome-level genome assembly of the flower thrips Frankliniella intonsa

As an economically important insect pest, the flower thrips Frankliniella intonsa (Trybom) causes great damage to host plants by directly feeding and indirectly transmitting various pathogenic viruses. The lack of a well-assembled genomic resource has hindered our understanding of the genetic basis...

Descripción completa

Detalles Bibliográficos
Autores principales: Zhang, Zhijun, Bao, Jiandong, Chen, Qizhang, He, Jianyun, Li, Xiaowei, Zhang, Jiahui, Liu, Zhixing, Wu, Yixuan, Li, Xuesheng, Wang, Yunsheng, Lu, Yaobin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Nature Publishing Group UK 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10689740/
https://www.ncbi.nlm.nih.gov/pubmed/38036569
http://dx.doi.org/10.1038/s41597-023-02770-3
_version_ 1785152413523509248
author Zhang, Zhijun
Bao, Jiandong
Chen, Qizhang
He, Jianyun
Li, Xiaowei
Zhang, Jiahui
Liu, Zhixing
Wu, Yixuan
Li, Xuesheng
Wang, Yunsheng
Lu, Yaobin
author_facet Zhang, Zhijun
Bao, Jiandong
Chen, Qizhang
He, Jianyun
Li, Xiaowei
Zhang, Jiahui
Liu, Zhixing
Wu, Yixuan
Li, Xuesheng
Wang, Yunsheng
Lu, Yaobin
author_sort Zhang, Zhijun
collection PubMed
description As an economically important insect pest, the flower thrips Frankliniella intonsa (Trybom) causes great damage to host plants by directly feeding and indirectly transmitting various pathogenic viruses. The lack of a well-assembled genomic resource has hindered our understanding of the genetic basis and evolution of F. intonsa. In this study, we used Oxford Nanopore Technology (ONT) long reads and High-through chromosome conformation capture (Hi-C) linked reads to construct a high-quality reference genome assembly of F. intonsa, with a total size of 225.5 Mb and a contig N50 of 3.37 Mb. By performing the Hi-C analysis, we anchored 91.68% of the contigs into 15 pseudochromosomes. Genomic annotation uncovered 17,581 protein-coding genes and identified 20.09% of the sequences as repeat elements. BUSCO analysis estimated over 98% of genome completeness. Our study is at the first time to report the chromosome-scale genome for the species of the genus Frankliniella. It provides a valuable genomic resource for further biological research and pest management of the thrips.
format Online
Article
Text
id pubmed-10689740
institution National Center for Biotechnology Information
language English
publishDate 2023
publisher Nature Publishing Group UK
record_format MEDLINE/PubMed
spelling pubmed-106897402023-12-02 Chromosome-level genome assembly of the flower thrips Frankliniella intonsa Zhang, Zhijun Bao, Jiandong Chen, Qizhang He, Jianyun Li, Xiaowei Zhang, Jiahui Liu, Zhixing Wu, Yixuan Li, Xuesheng Wang, Yunsheng Lu, Yaobin Sci Data Data Descriptor As an economically important insect pest, the flower thrips Frankliniella intonsa (Trybom) causes great damage to host plants by directly feeding and indirectly transmitting various pathogenic viruses. The lack of a well-assembled genomic resource has hindered our understanding of the genetic basis and evolution of F. intonsa. In this study, we used Oxford Nanopore Technology (ONT) long reads and High-through chromosome conformation capture (Hi-C) linked reads to construct a high-quality reference genome assembly of F. intonsa, with a total size of 225.5 Mb and a contig N50 of 3.37 Mb. By performing the Hi-C analysis, we anchored 91.68% of the contigs into 15 pseudochromosomes. Genomic annotation uncovered 17,581 protein-coding genes and identified 20.09% of the sequences as repeat elements. BUSCO analysis estimated over 98% of genome completeness. Our study is at the first time to report the chromosome-scale genome for the species of the genus Frankliniella. It provides a valuable genomic resource for further biological research and pest management of the thrips. Nature Publishing Group UK 2023-11-30 /pmc/articles/PMC10689740/ /pubmed/38036569 http://dx.doi.org/10.1038/s41597-023-02770-3 Text en © The Author(s) 2023 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Data Descriptor
Zhang, Zhijun
Bao, Jiandong
Chen, Qizhang
He, Jianyun
Li, Xiaowei
Zhang, Jiahui
Liu, Zhixing
Wu, Yixuan
Li, Xuesheng
Wang, Yunsheng
Lu, Yaobin
Chromosome-level genome assembly of the flower thrips Frankliniella intonsa
title Chromosome-level genome assembly of the flower thrips Frankliniella intonsa
title_full Chromosome-level genome assembly of the flower thrips Frankliniella intonsa
title_fullStr Chromosome-level genome assembly of the flower thrips Frankliniella intonsa
title_full_unstemmed Chromosome-level genome assembly of the flower thrips Frankliniella intonsa
title_short Chromosome-level genome assembly of the flower thrips Frankliniella intonsa
title_sort chromosome-level genome assembly of the flower thrips frankliniella intonsa
topic Data Descriptor
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10689740/
https://www.ncbi.nlm.nih.gov/pubmed/38036569
http://dx.doi.org/10.1038/s41597-023-02770-3
work_keys_str_mv AT zhangzhijun chromosomelevelgenomeassemblyoftheflowerthripsfrankliniellaintonsa
AT baojiandong chromosomelevelgenomeassemblyoftheflowerthripsfrankliniellaintonsa
AT chenqizhang chromosomelevelgenomeassemblyoftheflowerthripsfrankliniellaintonsa
AT hejianyun chromosomelevelgenomeassemblyoftheflowerthripsfrankliniellaintonsa
AT lixiaowei chromosomelevelgenomeassemblyoftheflowerthripsfrankliniellaintonsa
AT zhangjiahui chromosomelevelgenomeassemblyoftheflowerthripsfrankliniellaintonsa
AT liuzhixing chromosomelevelgenomeassemblyoftheflowerthripsfrankliniellaintonsa
AT wuyixuan chromosomelevelgenomeassemblyoftheflowerthripsfrankliniellaintonsa
AT lixuesheng chromosomelevelgenomeassemblyoftheflowerthripsfrankliniellaintonsa
AT wangyunsheng chromosomelevelgenomeassemblyoftheflowerthripsfrankliniellaintonsa
AT luyaobin chromosomelevelgenomeassemblyoftheflowerthripsfrankliniellaintonsa