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Chromosome-level genome assembly of the flower thrips Frankliniella intonsa
As an economically important insect pest, the flower thrips Frankliniella intonsa (Trybom) causes great damage to host plants by directly feeding and indirectly transmitting various pathogenic viruses. The lack of a well-assembled genomic resource has hindered our understanding of the genetic basis...
Autores principales: | , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10689740/ https://www.ncbi.nlm.nih.gov/pubmed/38036569 http://dx.doi.org/10.1038/s41597-023-02770-3 |
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author | Zhang, Zhijun Bao, Jiandong Chen, Qizhang He, Jianyun Li, Xiaowei Zhang, Jiahui Liu, Zhixing Wu, Yixuan Li, Xuesheng Wang, Yunsheng Lu, Yaobin |
author_facet | Zhang, Zhijun Bao, Jiandong Chen, Qizhang He, Jianyun Li, Xiaowei Zhang, Jiahui Liu, Zhixing Wu, Yixuan Li, Xuesheng Wang, Yunsheng Lu, Yaobin |
author_sort | Zhang, Zhijun |
collection | PubMed |
description | As an economically important insect pest, the flower thrips Frankliniella intonsa (Trybom) causes great damage to host plants by directly feeding and indirectly transmitting various pathogenic viruses. The lack of a well-assembled genomic resource has hindered our understanding of the genetic basis and evolution of F. intonsa. In this study, we used Oxford Nanopore Technology (ONT) long reads and High-through chromosome conformation capture (Hi-C) linked reads to construct a high-quality reference genome assembly of F. intonsa, with a total size of 225.5 Mb and a contig N50 of 3.37 Mb. By performing the Hi-C analysis, we anchored 91.68% of the contigs into 15 pseudochromosomes. Genomic annotation uncovered 17,581 protein-coding genes and identified 20.09% of the sequences as repeat elements. BUSCO analysis estimated over 98% of genome completeness. Our study is at the first time to report the chromosome-scale genome for the species of the genus Frankliniella. It provides a valuable genomic resource for further biological research and pest management of the thrips. |
format | Online Article Text |
id | pubmed-10689740 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-106897402023-12-02 Chromosome-level genome assembly of the flower thrips Frankliniella intonsa Zhang, Zhijun Bao, Jiandong Chen, Qizhang He, Jianyun Li, Xiaowei Zhang, Jiahui Liu, Zhixing Wu, Yixuan Li, Xuesheng Wang, Yunsheng Lu, Yaobin Sci Data Data Descriptor As an economically important insect pest, the flower thrips Frankliniella intonsa (Trybom) causes great damage to host plants by directly feeding and indirectly transmitting various pathogenic viruses. The lack of a well-assembled genomic resource has hindered our understanding of the genetic basis and evolution of F. intonsa. In this study, we used Oxford Nanopore Technology (ONT) long reads and High-through chromosome conformation capture (Hi-C) linked reads to construct a high-quality reference genome assembly of F. intonsa, with a total size of 225.5 Mb and a contig N50 of 3.37 Mb. By performing the Hi-C analysis, we anchored 91.68% of the contigs into 15 pseudochromosomes. Genomic annotation uncovered 17,581 protein-coding genes and identified 20.09% of the sequences as repeat elements. BUSCO analysis estimated over 98% of genome completeness. Our study is at the first time to report the chromosome-scale genome for the species of the genus Frankliniella. It provides a valuable genomic resource for further biological research and pest management of the thrips. Nature Publishing Group UK 2023-11-30 /pmc/articles/PMC10689740/ /pubmed/38036569 http://dx.doi.org/10.1038/s41597-023-02770-3 Text en © The Author(s) 2023 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Data Descriptor Zhang, Zhijun Bao, Jiandong Chen, Qizhang He, Jianyun Li, Xiaowei Zhang, Jiahui Liu, Zhixing Wu, Yixuan Li, Xuesheng Wang, Yunsheng Lu, Yaobin Chromosome-level genome assembly of the flower thrips Frankliniella intonsa |
title | Chromosome-level genome assembly of the flower thrips Frankliniella intonsa |
title_full | Chromosome-level genome assembly of the flower thrips Frankliniella intonsa |
title_fullStr | Chromosome-level genome assembly of the flower thrips Frankliniella intonsa |
title_full_unstemmed | Chromosome-level genome assembly of the flower thrips Frankliniella intonsa |
title_short | Chromosome-level genome assembly of the flower thrips Frankliniella intonsa |
title_sort | chromosome-level genome assembly of the flower thrips frankliniella intonsa |
topic | Data Descriptor |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10689740/ https://www.ncbi.nlm.nih.gov/pubmed/38036569 http://dx.doi.org/10.1038/s41597-023-02770-3 |
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