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Sex differences in brain transcriptomes of juvenile Cynomolgus macaques

Background: Behavioral, social, and physical characteristics are posited to distinguish the sexes, yet research on transcription-level sexual differences in the brain is limited. Here, we investigated sexually divergent brain transcriptomics in prepubertal cynomolgus macaques, a commonly used surrog...

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Autores principales: Kabbej, Nadia, Ashby, Frederick J., Riva, Alberto, Gamlin, Paul D., Mandel, Ronald J., Kunta, Aishwarya, Rouse, Courtney J., Heldermon, Coy D.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Journal Experts 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10690328/
https://www.ncbi.nlm.nih.gov/pubmed/38045237
http://dx.doi.org/10.21203/rs.3.rs-3422091/v1
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author Kabbej, Nadia
Ashby, Frederick J.
Riva, Alberto
Gamlin, Paul D.
Mandel, Ronald J.
Kunta, Aishwarya
Rouse, Courtney J.
Heldermon, Coy D.
author_facet Kabbej, Nadia
Ashby, Frederick J.
Riva, Alberto
Gamlin, Paul D.
Mandel, Ronald J.
Kunta, Aishwarya
Rouse, Courtney J.
Heldermon, Coy D.
author_sort Kabbej, Nadia
collection PubMed
description Background: Behavioral, social, and physical characteristics are posited to distinguish the sexes, yet research on transcription-level sexual differences in the brain is limited. Here, we investigated sexually divergent brain transcriptomics in prepubertal cynomolgus macaques, a commonly used surrogate species to humans. Methods: A transcriptomic profile using RNA sequencing was generated for the temporal lobe, ventral midbrain, and cerebellum of 3 female and 3 male cynomolgus macaques previously treated with an Adeno-associated virus vector mix. Statistical analyses to determine differentially expressed protein-coding genes in all three lobes were conducted using DeSeq2 with a false discovery rate corrected P value of .05. Results: We identified target genes in the temporal lobe, ventral midbrain, and cerebellum with functions in translation, immunity, behavior, and neurological disorders that exhibited statistically significant sexually divergent expression. Conclusions: We provide potential mechanistic insights to the epidemiological differences observed between the sexes with regards to mental health and infectious diseases, such as COVID19. Our results provide pre-pubertal information on sexual differences in non-human primate brain transcriptomics and may provide insight to health disparities between the biological sexes in humans.
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spelling pubmed-106903282023-12-02 Sex differences in brain transcriptomes of juvenile Cynomolgus macaques Kabbej, Nadia Ashby, Frederick J. Riva, Alberto Gamlin, Paul D. Mandel, Ronald J. Kunta, Aishwarya Rouse, Courtney J. Heldermon, Coy D. Res Sq Article Background: Behavioral, social, and physical characteristics are posited to distinguish the sexes, yet research on transcription-level sexual differences in the brain is limited. Here, we investigated sexually divergent brain transcriptomics in prepubertal cynomolgus macaques, a commonly used surrogate species to humans. Methods: A transcriptomic profile using RNA sequencing was generated for the temporal lobe, ventral midbrain, and cerebellum of 3 female and 3 male cynomolgus macaques previously treated with an Adeno-associated virus vector mix. Statistical analyses to determine differentially expressed protein-coding genes in all three lobes were conducted using DeSeq2 with a false discovery rate corrected P value of .05. Results: We identified target genes in the temporal lobe, ventral midbrain, and cerebellum with functions in translation, immunity, behavior, and neurological disorders that exhibited statistically significant sexually divergent expression. Conclusions: We provide potential mechanistic insights to the epidemiological differences observed between the sexes with regards to mental health and infectious diseases, such as COVID19. Our results provide pre-pubertal information on sexual differences in non-human primate brain transcriptomics and may provide insight to health disparities between the biological sexes in humans. American Journal Experts 2023-11-20 /pmc/articles/PMC10690328/ /pubmed/38045237 http://dx.doi.org/10.21203/rs.3.rs-3422091/v1 Text en https://creativecommons.org/licenses/by/4.0/This work is licensed under a Creative Commons Attribution 4.0 International License (https://creativecommons.org/licenses/by/4.0/) , which allows reusers to distribute, remix, adapt, and build upon the material in any medium or format, so long as attribution is given to the creator. The license allows for commercial use.
spellingShingle Article
Kabbej, Nadia
Ashby, Frederick J.
Riva, Alberto
Gamlin, Paul D.
Mandel, Ronald J.
Kunta, Aishwarya
Rouse, Courtney J.
Heldermon, Coy D.
Sex differences in brain transcriptomes of juvenile Cynomolgus macaques
title Sex differences in brain transcriptomes of juvenile Cynomolgus macaques
title_full Sex differences in brain transcriptomes of juvenile Cynomolgus macaques
title_fullStr Sex differences in brain transcriptomes of juvenile Cynomolgus macaques
title_full_unstemmed Sex differences in brain transcriptomes of juvenile Cynomolgus macaques
title_short Sex differences in brain transcriptomes of juvenile Cynomolgus macaques
title_sort sex differences in brain transcriptomes of juvenile cynomolgus macaques
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10690328/
https://www.ncbi.nlm.nih.gov/pubmed/38045237
http://dx.doi.org/10.21203/rs.3.rs-3422091/v1
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