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Phenotype plasticity and altered sensitivity to chemotherapeutic agents in aggressive prostate cancer cells

In 2023, approximately 288,300 new diagnoses of prostate cancer will occur, with 34,700 disease-related deaths. Death from prostate cancer is associated with metastasis, enabled by progression of tumor phenotypes and successful extracapsular extension to reach Batson’s venous plexus, a specific rout...

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Autores principales: Paxson, Allan I., Chang, Loren H., Gard, Jaime M. C., Harryman, William L., Nelson, Colin S., Salmon, Stella B., Marr, Kendra D., Wachsmuth, Leah M., Ramanathan, Anita, Ran, Jing, Kapoor, Abhijeet, Marugan, Juan J., Henderson, Mark J., Sanchez, Tino W., Cress, Anne E.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10690371/
https://www.ncbi.nlm.nih.gov/pubmed/38046670
http://dx.doi.org/10.3389/fcell.2023.1285372
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author Paxson, Allan I.
Chang, Loren H.
Gard, Jaime M. C.
Harryman, William L.
Nelson, Colin S.
Salmon, Stella B.
Marr, Kendra D.
Wachsmuth, Leah M.
Ramanathan, Anita
Ran, Jing
Kapoor, Abhijeet
Marugan, Juan J.
Henderson, Mark J.
Sanchez, Tino W.
Cress, Anne E.
author_facet Paxson, Allan I.
Chang, Loren H.
Gard, Jaime M. C.
Harryman, William L.
Nelson, Colin S.
Salmon, Stella B.
Marr, Kendra D.
Wachsmuth, Leah M.
Ramanathan, Anita
Ran, Jing
Kapoor, Abhijeet
Marugan, Juan J.
Henderson, Mark J.
Sanchez, Tino W.
Cress, Anne E.
author_sort Paxson, Allan I.
collection PubMed
description In 2023, approximately 288,300 new diagnoses of prostate cancer will occur, with 34,700 disease-related deaths. Death from prostate cancer is associated with metastasis, enabled by progression of tumor phenotypes and successful extracapsular extension to reach Batson’s venous plexus, a specific route to the spine and brain. Using a mouse-human tumor xenograft model, we isolated an aggressive muscle invasive cell population of prostate cancer, called DU145(J7) with a distinct biophysical phenotype, elevated histone H3K27, and increased matrix metalloproteinase 14 expression as compared to the non-aggressive parent cell population called DU145(WT). Our goal was to determine the sensitivities to known chemotherapeutic agents of the aggressive cells as compared to the parent population. High-throughput screening was performed with 5,578 compounds, comprising of approved and investigational drugs for oncology. Eleven compounds were selected for additional testing, which revealed that vorinostat, 5-azacitidine, and fimepinostat (epigenetic inhibitors) showed 2.6-to-7.5-fold increases in lethality for the aggressive prostate cancer cell population as compared to the parent, as judged by the concentration of drug to inhibit 50% cell growth (IC(50)). On the other hand, the DU145(J7) cells were 2.2-to-4.0-fold resistant to mitoxantrone, daunorubicin, and gimatecan (topoisomerase inhibitors) as compared to DU145(WT). No differences in sensitivities between cell populations were found for docetaxel or pirarubicin. The increased sensitivity of DU145(J7) prostate cancer cells to chromatin modifying agents suggests a therapeutic vulnerability occurs after tumor cells invade into and through muscle. Future work will determine which epigenetic modifiers and what combinations will be most effective to eradicate early aggressive tumor populations.
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spelling pubmed-106903712023-12-02 Phenotype plasticity and altered sensitivity to chemotherapeutic agents in aggressive prostate cancer cells Paxson, Allan I. Chang, Loren H. Gard, Jaime M. C. Harryman, William L. Nelson, Colin S. Salmon, Stella B. Marr, Kendra D. Wachsmuth, Leah M. Ramanathan, Anita Ran, Jing Kapoor, Abhijeet Marugan, Juan J. Henderson, Mark J. Sanchez, Tino W. Cress, Anne E. Front Cell Dev Biol Cell and Developmental Biology In 2023, approximately 288,300 new diagnoses of prostate cancer will occur, with 34,700 disease-related deaths. Death from prostate cancer is associated with metastasis, enabled by progression of tumor phenotypes and successful extracapsular extension to reach Batson’s venous plexus, a specific route to the spine and brain. Using a mouse-human tumor xenograft model, we isolated an aggressive muscle invasive cell population of prostate cancer, called DU145(J7) with a distinct biophysical phenotype, elevated histone H3K27, and increased matrix metalloproteinase 14 expression as compared to the non-aggressive parent cell population called DU145(WT). Our goal was to determine the sensitivities to known chemotherapeutic agents of the aggressive cells as compared to the parent population. High-throughput screening was performed with 5,578 compounds, comprising of approved and investigational drugs for oncology. Eleven compounds were selected for additional testing, which revealed that vorinostat, 5-azacitidine, and fimepinostat (epigenetic inhibitors) showed 2.6-to-7.5-fold increases in lethality for the aggressive prostate cancer cell population as compared to the parent, as judged by the concentration of drug to inhibit 50% cell growth (IC(50)). On the other hand, the DU145(J7) cells were 2.2-to-4.0-fold resistant to mitoxantrone, daunorubicin, and gimatecan (topoisomerase inhibitors) as compared to DU145(WT). No differences in sensitivities between cell populations were found for docetaxel or pirarubicin. The increased sensitivity of DU145(J7) prostate cancer cells to chromatin modifying agents suggests a therapeutic vulnerability occurs after tumor cells invade into and through muscle. Future work will determine which epigenetic modifiers and what combinations will be most effective to eradicate early aggressive tumor populations. Frontiers Media S.A. 2023-11-16 /pmc/articles/PMC10690371/ /pubmed/38046670 http://dx.doi.org/10.3389/fcell.2023.1285372 Text en Copyright © 2023 Paxson, Chang, Gard, Harryman, Nelson, Salmon, Marr, Wachsmuth, Ramanathan, Ran, Kapoor, Marugan, Henderson, Sanchez and Cress. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Cell and Developmental Biology
Paxson, Allan I.
Chang, Loren H.
Gard, Jaime M. C.
Harryman, William L.
Nelson, Colin S.
Salmon, Stella B.
Marr, Kendra D.
Wachsmuth, Leah M.
Ramanathan, Anita
Ran, Jing
Kapoor, Abhijeet
Marugan, Juan J.
Henderson, Mark J.
Sanchez, Tino W.
Cress, Anne E.
Phenotype plasticity and altered sensitivity to chemotherapeutic agents in aggressive prostate cancer cells
title Phenotype plasticity and altered sensitivity to chemotherapeutic agents in aggressive prostate cancer cells
title_full Phenotype plasticity and altered sensitivity to chemotherapeutic agents in aggressive prostate cancer cells
title_fullStr Phenotype plasticity and altered sensitivity to chemotherapeutic agents in aggressive prostate cancer cells
title_full_unstemmed Phenotype plasticity and altered sensitivity to chemotherapeutic agents in aggressive prostate cancer cells
title_short Phenotype plasticity and altered sensitivity to chemotherapeutic agents in aggressive prostate cancer cells
title_sort phenotype plasticity and altered sensitivity to chemotherapeutic agents in aggressive prostate cancer cells
topic Cell and Developmental Biology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10690371/
https://www.ncbi.nlm.nih.gov/pubmed/38046670
http://dx.doi.org/10.3389/fcell.2023.1285372
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