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High-dimensional mass cytometry reveals systemic and local immune signatures in necrotizing enterocolitis
OBJECTIVE: Patients with necrotizing enterocolitis display severe gastrointestinal complications of prematurity, but the mechanism driving this clinical profile remains unknown. We used mass cytometry time-of-flight to characterize and compare immune cell populations in the blood and intestine tissu...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2023
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10690805/ https://www.ncbi.nlm.nih.gov/pubmed/38045686 http://dx.doi.org/10.3389/fimmu.2023.1292987 |
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author | Liu, Yufeng Zhou, Jialiang Chen, Baozhu Liu, Xiao Cai, Yao Liu, Wei Hao, Hu Li, Sitao |
author_facet | Liu, Yufeng Zhou, Jialiang Chen, Baozhu Liu, Xiao Cai, Yao Liu, Wei Hao, Hu Li, Sitao |
author_sort | Liu, Yufeng |
collection | PubMed |
description | OBJECTIVE: Patients with necrotizing enterocolitis display severe gastrointestinal complications of prematurity, but the mechanism driving this clinical profile remains unknown. We used mass cytometry time-of-flight to characterize and compare immune cell populations in the blood and intestine tissue from patients with and without (controls) necrotizing enterocolitis at single-cell resolution. METHODS: We completed a deep mapping of the immune system of the peripheral blood mononuclear cells and intestinal mucosa tissue using mass cytometry to evaluate immune cell types, which revealed global immune dysregulation characteristics underlying necrotizing enterocolitis. RESULTS: Compared with controls, natural killer cells display signs of heightened activation and increased cytotoxic potential in the peripheral blood and mucosa of patients with necrotizing enterocolitis. Furthermore, CD4(+) T effector memory cells, non-classical monocytes, active dendritic cells, and neutrophils were specifically enriched in the mucosa, suggesting trafficking from the periphery to areas of inflammation. Moreover, we mapped the systemic and local distinct immune signatures suggesting patterns of cell localization in necrotizing enterocolitis. CONCLUSION: We used mass cytometry time-of-flight technology to identify immune cell populations specific to the peripheral blood and intestinal mucosa tissue from patients with necrotizing enterocolitis and controls. This information might be used to develop precise diagnosis and therapies that target specific cell populations in patients with necrotizing enterocolitis. |
format | Online Article Text |
id | pubmed-10690805 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-106908052023-12-02 High-dimensional mass cytometry reveals systemic and local immune signatures in necrotizing enterocolitis Liu, Yufeng Zhou, Jialiang Chen, Baozhu Liu, Xiao Cai, Yao Liu, Wei Hao, Hu Li, Sitao Front Immunol Immunology OBJECTIVE: Patients with necrotizing enterocolitis display severe gastrointestinal complications of prematurity, but the mechanism driving this clinical profile remains unknown. We used mass cytometry time-of-flight to characterize and compare immune cell populations in the blood and intestine tissue from patients with and without (controls) necrotizing enterocolitis at single-cell resolution. METHODS: We completed a deep mapping of the immune system of the peripheral blood mononuclear cells and intestinal mucosa tissue using mass cytometry to evaluate immune cell types, which revealed global immune dysregulation characteristics underlying necrotizing enterocolitis. RESULTS: Compared with controls, natural killer cells display signs of heightened activation and increased cytotoxic potential in the peripheral blood and mucosa of patients with necrotizing enterocolitis. Furthermore, CD4(+) T effector memory cells, non-classical monocytes, active dendritic cells, and neutrophils were specifically enriched in the mucosa, suggesting trafficking from the periphery to areas of inflammation. Moreover, we mapped the systemic and local distinct immune signatures suggesting patterns of cell localization in necrotizing enterocolitis. CONCLUSION: We used mass cytometry time-of-flight technology to identify immune cell populations specific to the peripheral blood and intestinal mucosa tissue from patients with necrotizing enterocolitis and controls. This information might be used to develop precise diagnosis and therapies that target specific cell populations in patients with necrotizing enterocolitis. Frontiers Media S.A. 2023-11-17 /pmc/articles/PMC10690805/ /pubmed/38045686 http://dx.doi.org/10.3389/fimmu.2023.1292987 Text en Copyright © 2023 Liu, Zhou, Chen, Liu, Cai, Liu, Hao and Li https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Immunology Liu, Yufeng Zhou, Jialiang Chen, Baozhu Liu, Xiao Cai, Yao Liu, Wei Hao, Hu Li, Sitao High-dimensional mass cytometry reveals systemic and local immune signatures in necrotizing enterocolitis |
title | High-dimensional mass cytometry reveals systemic and local immune signatures in necrotizing enterocolitis |
title_full | High-dimensional mass cytometry reveals systemic and local immune signatures in necrotizing enterocolitis |
title_fullStr | High-dimensional mass cytometry reveals systemic and local immune signatures in necrotizing enterocolitis |
title_full_unstemmed | High-dimensional mass cytometry reveals systemic and local immune signatures in necrotizing enterocolitis |
title_short | High-dimensional mass cytometry reveals systemic and local immune signatures in necrotizing enterocolitis |
title_sort | high-dimensional mass cytometry reveals systemic and local immune signatures in necrotizing enterocolitis |
topic | Immunology |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10690805/ https://www.ncbi.nlm.nih.gov/pubmed/38045686 http://dx.doi.org/10.3389/fimmu.2023.1292987 |
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