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Microbial interaction-induced siderophore dynamics lead to phenotypic differentiation of Staphylococcus aureus

This study investigated the impact of microbial interactions on siderophore dynamics and phenotypic differentiation of Staphylococcus aureus under iron-deficient conditions. Optimization of media demonstrated that the glycerol alanine salts medium was best suited for analyzing the dynamics of sidero...

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Autores principales: Rajapitamahuni, Soundarya, Lyou, Eun Sun, Kang, Bo Ram, Lee, Tae Kwon
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10690949/
https://www.ncbi.nlm.nih.gov/pubmed/38045757
http://dx.doi.org/10.3389/fcimb.2023.1277176
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author Rajapitamahuni, Soundarya
Lyou, Eun Sun
Kang, Bo Ram
Lee, Tae Kwon
author_facet Rajapitamahuni, Soundarya
Lyou, Eun Sun
Kang, Bo Ram
Lee, Tae Kwon
author_sort Rajapitamahuni, Soundarya
collection PubMed
description This study investigated the impact of microbial interactions on siderophore dynamics and phenotypic differentiation of Staphylococcus aureus under iron-deficient conditions. Optimization of media demonstrated that the glycerol alanine salts medium was best suited for analyzing the dynamics of siderophore production because of its stable production of diverse siderophore types. The effects of pH and iron concentration on siderophore yield revealed a maximum yield at neutral pH and low iron concentration (10 µg). Microbial interaction studies have highlighted variations in siderophore production when different strains (Staphylococcus epidermidis, Pseudomonas aeruginosa, and Escherichia coli) are co-cultured with S. aureus. Co-culture of S. aureus with P. aeruginosa eliminated siderophore production in S. aureus, while co-culture of S. aureus with E. coli and S. epidermidis produced one or two siderophores, respectively. Raman spectroscopy revealed that microbial interactions and siderophore dynamics play a crucial role in directing the phenotypic differentiation of S. aureus, especially under iron-deficient conditions. Our results suggest that microbial interactions profoundly influence siderophore dynamics and phenotypic differentiation and that the study of these interactions could provide valuable insights for understanding microbial survival strategies in iron-limited environments.
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spelling pubmed-106909492023-12-02 Microbial interaction-induced siderophore dynamics lead to phenotypic differentiation of Staphylococcus aureus Rajapitamahuni, Soundarya Lyou, Eun Sun Kang, Bo Ram Lee, Tae Kwon Front Cell Infect Microbiol Cellular and Infection Microbiology This study investigated the impact of microbial interactions on siderophore dynamics and phenotypic differentiation of Staphylococcus aureus under iron-deficient conditions. Optimization of media demonstrated that the glycerol alanine salts medium was best suited for analyzing the dynamics of siderophore production because of its stable production of diverse siderophore types. The effects of pH and iron concentration on siderophore yield revealed a maximum yield at neutral pH and low iron concentration (10 µg). Microbial interaction studies have highlighted variations in siderophore production when different strains (Staphylococcus epidermidis, Pseudomonas aeruginosa, and Escherichia coli) are co-cultured with S. aureus. Co-culture of S. aureus with P. aeruginosa eliminated siderophore production in S. aureus, while co-culture of S. aureus with E. coli and S. epidermidis produced one or two siderophores, respectively. Raman spectroscopy revealed that microbial interactions and siderophore dynamics play a crucial role in directing the phenotypic differentiation of S. aureus, especially under iron-deficient conditions. Our results suggest that microbial interactions profoundly influence siderophore dynamics and phenotypic differentiation and that the study of these interactions could provide valuable insights for understanding microbial survival strategies in iron-limited environments. Frontiers Media S.A. 2023-11-17 /pmc/articles/PMC10690949/ /pubmed/38045757 http://dx.doi.org/10.3389/fcimb.2023.1277176 Text en Copyright © 2023 Rajapitamahuni, Lyou, Kang and Lee https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Cellular and Infection Microbiology
Rajapitamahuni, Soundarya
Lyou, Eun Sun
Kang, Bo Ram
Lee, Tae Kwon
Microbial interaction-induced siderophore dynamics lead to phenotypic differentiation of Staphylococcus aureus
title Microbial interaction-induced siderophore dynamics lead to phenotypic differentiation of Staphylococcus aureus
title_full Microbial interaction-induced siderophore dynamics lead to phenotypic differentiation of Staphylococcus aureus
title_fullStr Microbial interaction-induced siderophore dynamics lead to phenotypic differentiation of Staphylococcus aureus
title_full_unstemmed Microbial interaction-induced siderophore dynamics lead to phenotypic differentiation of Staphylococcus aureus
title_short Microbial interaction-induced siderophore dynamics lead to phenotypic differentiation of Staphylococcus aureus
title_sort microbial interaction-induced siderophore dynamics lead to phenotypic differentiation of staphylococcus aureus
topic Cellular and Infection Microbiology
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10690949/
https://www.ncbi.nlm.nih.gov/pubmed/38045757
http://dx.doi.org/10.3389/fcimb.2023.1277176
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