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Histological characteristics of hair follicles at different hair cycle and in vitro modeling of hair follicle-associated cells of yak (Bos grunniens)

To adapt to the extreme conditions of plateau environments, yaks have evolved thick hair, making them an ideal model for investigating the mechanisms involved in hair growth. We can gain valuable insights into how hair follicles develop and their cyclic growth in challenging environments by studying...

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Autores principales: Liao, Bo, Cui, Yan, Yu, Sijiu, He, Junfeng, Yang, Xue, Zou, Shengnan, Li, Sijie, Zhao, Pengfei, Xu, Hongwei, Long, Min, Wang, Xiaoyan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Frontiers Media S.A. 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10691264/
https://www.ncbi.nlm.nih.gov/pubmed/38046572
http://dx.doi.org/10.3389/fvets.2023.1277586
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author Liao, Bo
Cui, Yan
Yu, Sijiu
He, Junfeng
Yang, Xue
Zou, Shengnan
Li, Sijie
Zhao, Pengfei
Xu, Hongwei
Long, Min
Wang, Xiaoyan
author_facet Liao, Bo
Cui, Yan
Yu, Sijiu
He, Junfeng
Yang, Xue
Zou, Shengnan
Li, Sijie
Zhao, Pengfei
Xu, Hongwei
Long, Min
Wang, Xiaoyan
author_sort Liao, Bo
collection PubMed
description To adapt to the extreme conditions of plateau environments, yaks have evolved thick hair, making them an ideal model for investigating the mechanisms involved in hair growth. We can gain valuable insights into how hair follicles develop and their cyclic growth in challenging environments by studying yaks. However, the lack of essential data on yak hair follicle histology and the absence of in vitro cell models for hair follicles serve as a limitation to such research objectives. In this study, we investigated the structure of skin tissue during different hair follicle cycles using the yak model. Additionally, we successfully established in vitro models of hair follicle-associated cells derived from yak skin, including dermal papilla cells (DPCs), preadipocytes, and fibroblasts. We optimized the microdissection technique for DPCs culture by simplifying the procedure and reducing the time required. Furthermore, we improved the methodology used to differentiate yak preadipocytes into mature adipocytes, thus increasing the differentiation efficiency. The introduction of yak as a natural model provides valuable research resources for exploring the mechanisms of hair growth and contributes to a deeper understanding of hair follicle biology and the development of regenerative medicine strategies.
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spelling pubmed-106912642023-12-02 Histological characteristics of hair follicles at different hair cycle and in vitro modeling of hair follicle-associated cells of yak (Bos grunniens) Liao, Bo Cui, Yan Yu, Sijiu He, Junfeng Yang, Xue Zou, Shengnan Li, Sijie Zhao, Pengfei Xu, Hongwei Long, Min Wang, Xiaoyan Front Vet Sci Veterinary Science To adapt to the extreme conditions of plateau environments, yaks have evolved thick hair, making them an ideal model for investigating the mechanisms involved in hair growth. We can gain valuable insights into how hair follicles develop and their cyclic growth in challenging environments by studying yaks. However, the lack of essential data on yak hair follicle histology and the absence of in vitro cell models for hair follicles serve as a limitation to such research objectives. In this study, we investigated the structure of skin tissue during different hair follicle cycles using the yak model. Additionally, we successfully established in vitro models of hair follicle-associated cells derived from yak skin, including dermal papilla cells (DPCs), preadipocytes, and fibroblasts. We optimized the microdissection technique for DPCs culture by simplifying the procedure and reducing the time required. Furthermore, we improved the methodology used to differentiate yak preadipocytes into mature adipocytes, thus increasing the differentiation efficiency. The introduction of yak as a natural model provides valuable research resources for exploring the mechanisms of hair growth and contributes to a deeper understanding of hair follicle biology and the development of regenerative medicine strategies. Frontiers Media S.A. 2023-11-17 /pmc/articles/PMC10691264/ /pubmed/38046572 http://dx.doi.org/10.3389/fvets.2023.1277586 Text en Copyright © 2023 Liao, Cui, Yu, He, Yang, Zou, Li, Zhao, Xu, Long and Wang. https://creativecommons.org/licenses/by/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
spellingShingle Veterinary Science
Liao, Bo
Cui, Yan
Yu, Sijiu
He, Junfeng
Yang, Xue
Zou, Shengnan
Li, Sijie
Zhao, Pengfei
Xu, Hongwei
Long, Min
Wang, Xiaoyan
Histological characteristics of hair follicles at different hair cycle and in vitro modeling of hair follicle-associated cells of yak (Bos grunniens)
title Histological characteristics of hair follicles at different hair cycle and in vitro modeling of hair follicle-associated cells of yak (Bos grunniens)
title_full Histological characteristics of hair follicles at different hair cycle and in vitro modeling of hair follicle-associated cells of yak (Bos grunniens)
title_fullStr Histological characteristics of hair follicles at different hair cycle and in vitro modeling of hair follicle-associated cells of yak (Bos grunniens)
title_full_unstemmed Histological characteristics of hair follicles at different hair cycle and in vitro modeling of hair follicle-associated cells of yak (Bos grunniens)
title_short Histological characteristics of hair follicles at different hair cycle and in vitro modeling of hair follicle-associated cells of yak (Bos grunniens)
title_sort histological characteristics of hair follicles at different hair cycle and in vitro modeling of hair follicle-associated cells of yak (bos grunniens)
topic Veterinary Science
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10691264/
https://www.ncbi.nlm.nih.gov/pubmed/38046572
http://dx.doi.org/10.3389/fvets.2023.1277586
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