Cargando…

Conditional and inducible transgene expression in mice through the combinatorial use of Cre-mediated recombination and tetracycline induction

Mostrar otras versiones (1)

Here we describe a triple transgenic mouse system, which combines the tissue specificity of any Cre-transgenic line with the inducibility of the reverse tetracycline transactivator (rtTA)/tetracycline-responsive element (tet-O)-driven transgenes. To ensure reliable rtTA expression in a broad range o...

Descripción completa

Detalles Bibliográficos
Autores principales:	Belteki, Gusztav, Haigh, Jody, Kabacs, Nikolett, Haigh, Katharina, Sison, Karen, Costantini, Frank, Whitsett, Jeff, Quaggin, Susan E., Nagy, Andras
Formato:	Texto
Lenguaje:	English
Publicado:	Oxford University Press 2005
Materias:	Methods Online
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC1069131/ https://www.ncbi.nlm.nih.gov/pubmed/15784609 http://dx.doi.org/10.1093/nar/gni051

Ejemplares similares

Conditional and inducible transgene expression in mice through the combinatorial use of Cre-mediated recombination and tetracycline induction
por: Belteki, Gusztav, et al.
Publicado: (2005)

Cre reconstitution allows for DNA recombination selectively in dual-marker-expressing cells in transgenic mice
por: Xu, Yanwen, et al.
Publicado: (2007)

Efficient mapping of transgene integration sites and local structural changes in Cre transgenic mice using targeted locus amplification
por: Cain-Hom, Carol, et al.
Publicado: (2017)

Efficient mouse transgenesis using Gateway-compatible ROSA26 locus targeting vectors and F1 hybrid ES cells
por: Nyabi, Omar, et al.
Publicado: (2009)

Reproducible doxycycline-inducible transgene expression at specific loci generated by Cre-recombinase mediated cassette exchange
por: Wong, Ee Tsin, et al.
Publicado: (2005)

Illumina mate-paired DNA sequencing-library preparation using Cre-Lox recombination
por: Van Nieuwerburgh, Filip, et al.
Publicado: (2012)

Iterative in vivo assembly of large and complex transgenes by combining the activities of φC31 integrase and Cre recombinase
por: Dafhnis-Calas, Felix, et al.
Publicado: (2005)

Combinatorial expression vector engineering for tuning of recombinant protein production in Escherichia coli
por: Bandmann, Nina, et al.
Publicado: (2007)

Vika/vox, a novel efficient and specific Cre/loxP-like site-specific recombination system
por: Karimova, Madina, et al.
Publicado: (2013)

Flp and Cre expressed from Flp–2A–Cre and Flp–IRES–Cre transcription units mediate the highest level of dual recombinase-mediated cassette exchange
por: Anderson, Rachelle P., et al.
Publicado: (2012)

Baculoviral transduction facilitates TALEN-mediated targeted transgene integration and Cre/LoxP cassette exchange in human-induced pluripotent stem cells
por: Zhu, Haibao, et al.
Publicado: (2013)

Tetracycline-inducible gene regulation in mycobacteria
por: Blokpoel, Marian C. J., et al.
Publicado: (2005)

High-level transgene expression by homologous recombination-mediated gene transfer
por: Grandjean, Mélanie, et al.
Publicado: (2011)

iFLinkC: an iterative functional linker cloning strategy for the combinatorial assembly and recombination of linker peptides with functional domains
por: Gräwe, Alexander, et al.
Publicado: (2020)

Single molecule analysis of combinatorial splicing
por: Conze, Tim, et al.
Publicado: (2010)

Integrative elements for Bacillus subtilis yielding tetracycline-dependent growth phenotypes
por: Bertram, Ralph, et al.
Publicado: (2005)

Natural and synthetic tetracycline-inducible promoters for use in the antibiotic-producing bacteria Streptomyces
por: Rodríguez-García, Antonio, et al.
Publicado: (2005)

pBT, a novel vector for tetracycline-regulated yeast three-hybrid assay
por: Moriyoshi, Koki
Publicado: (2009)

Customized optimization of metabolic pathways by combinatorial transcriptional engineering
por: Du, Jing, et al.
Publicado: (2012)

Expression of Cre recombinase during transient phage infection permits efficient marker removal in Streptomyces
por: Khodakaramian, Gholam, et al.
Publicado: (2006)

Conditional brain-specific knockdown of MAPK using Cre/loxP regulated RNA interference
por: Hitz, Christiane, et al.
Publicado: (2007)

Conditional knockdown of Fgfr2 in mice using Cre-LoxP induced RNA interference
por: Coumoul, Xavier, et al.
Publicado: (2005)

Quantitative analysis of conditional gene inactivation using rationally designed, tetracycline-controlled miRNAs
por: Berger, Stefan M., et al.
Publicado: (2010)

Decoding the genome with an integrative analysis tool: Combinatorial CRM Decoder
por: Kang, Keunsoo, et al.
Publicado: (2011)

Yeast two-hybrid interaction partner screening through in vivo Cre-mediated Binary Interaction Tag generation
por: Hastie, Alex R., et al.
Publicado: (2007)

Efficient splicing-based RNA regulators for tetracycline-inducible gene expression in human cell culture and C. elegans
por: Finke, Monika, et al.
Publicado: (2021)

A combinatorial approach to create artificial homing endonucleases cleaving chosen sequences
por: Smith, Julianne, et al.
Publicado: (2006)

A combinatorial method to isolate short ribozymes from complex ribozyme libraries
por: Arriola, Joshua T, et al.
Publicado: (2020)

Combinatorial assembly platform enabling engineering of genetically stable metabolic pathways in cyanobacteria
por: Taylor, George M, et al.
Publicado: (2021)

Comparison of RNAi efficiency mediated by tetracycline-responsive H1 and U6 promoter variants in mammalian cell lines
por: Henriksen, Jørn R., et al.
Publicado: (2007)

One step construction of PCR mutagenized libraries for genetic analysis by recombination cloning
por: Khalil, Al-Muataz, et al.
Publicado: (2007)

Optimizing nucleotide sequence ensembles for combinatorial protein libraries using a genetic algorithm
por: Craig, Roger A., et al.
Publicado: (2010)

Achieving tight control of a photoactivatable Cre recombinase gene switch: new design strategies and functional characterization in mammalian cells and rodent
por: Meador, Kyle, et al.
Publicado: (2019)

Site-specific recombination in Schizosaccharomyces pombe and systematic assembly of a 400kb transgene array in mammalian cells using the integrase of Streptomyces phage ϕBT1
por: Xu, Zhengyao, et al.
Publicado: (2008)

Inducible and tissue‐specific cell labeling in Cre‐ER ( T2 ) transgenic Xenopus lines
por: Lin, Tzi‐Yang, et al.
Publicado: (2022)

Elucidating combinatorial histone modifications and crosstalks by coupling histone-modifying enzyme with biotin ligase activity
por: Lau, Priscilla Nga Ieng, et al.
Publicado: (2013)

Incorporation of aptamers in the terminal loop of shRNAs yields an effective and novel combinatorial targeting strategy
por: Pang, Ka Ming, et al.
Publicado: (2018)

SLICE: determining cell differentiation and lineage based on single cell entropy
por: Guo, Minzhe, et al.
Publicado: (2017)

Unveiling combinatorial regulation through the combination of ChIP information and in silico cis-regulatory module detection
por: Sun, Hong, et al.
Publicado: (2012)

Network controllability-based algorithm to target personalized driver genes for discovering combinatorial drugs of individual patients
por: Guo, Wei-Feng, et al.
Publicado: (2021)

Cannot write session to /tmp/vufind_sessions/sess_loojufutabhfs6d721vk4norj1