Cargando…
A novel long noncoding RNA (lncRNA), LINC02657(LASTR), is a prognostic biomarker associated with immune infiltrates of lung adenocarcinoma based on unsupervised cluster analysis
Non-small cell lung cancer (NSCLC) has long been the deadliest malignancy worldwide, with adenocarcinoma (AD) being the most common pathological subtype. Here we focused on the value of LASTR in LUAD. Using expression analysis, enrichment analysis, immune cell infraction analysis, we found that the...
Autores principales: | , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
PeerJ Inc.
2023
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10691363/ https://www.ncbi.nlm.nih.gov/pubmed/38047034 http://dx.doi.org/10.7717/peerj.16167 |
_version_ | 1785152723375620096 |
---|---|
author | Kong, Fanming Yang, Xinyu Lu, Zhichao Liu, Zongheng Yang, Yang Wang, Ziheng |
author_facet | Kong, Fanming Yang, Xinyu Lu, Zhichao Liu, Zongheng Yang, Yang Wang, Ziheng |
author_sort | Kong, Fanming |
collection | PubMed |
description | Non-small cell lung cancer (NSCLC) has long been the deadliest malignancy worldwide, with adenocarcinoma (AD) being the most common pathological subtype. Here we focused on the value of LASTR in LUAD. Using expression analysis, enrichment analysis, immune cell infraction analysis, we found that the expression level of LASTR was significantly increased in LUAD tissue. Meanwhile, LASTR was significantly associated with differential infiltration of various immune cells. Kaplan-Meier survival analysis showed that LUAD related with a poor prognosis in terms of OS, PFI, and DSS compared with high-expression LASTR. The enrichment analysis showed that LASTR is related to the pathays like PI3K-AKT signaling pathway. Thus, the present findings could be helpful in a better understand of LASTR in LUAD. RT-PCR was used to verify the high expression of LASTR in LUAD tissues, and the apoptosis of LUAD cell lines was promoted by CCK8 and Transwell experiments to verify the ability of LASTR to promote the migration and invasion of lung cancer cells in vitro. |
format | Online Article Text |
id | pubmed-10691363 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | PeerJ Inc. |
record_format | MEDLINE/PubMed |
spelling | pubmed-106913632023-12-02 A novel long noncoding RNA (lncRNA), LINC02657(LASTR), is a prognostic biomarker associated with immune infiltrates of lung adenocarcinoma based on unsupervised cluster analysis Kong, Fanming Yang, Xinyu Lu, Zhichao Liu, Zongheng Yang, Yang Wang, Ziheng PeerJ Bioinformatics Non-small cell lung cancer (NSCLC) has long been the deadliest malignancy worldwide, with adenocarcinoma (AD) being the most common pathological subtype. Here we focused on the value of LASTR in LUAD. Using expression analysis, enrichment analysis, immune cell infraction analysis, we found that the expression level of LASTR was significantly increased in LUAD tissue. Meanwhile, LASTR was significantly associated with differential infiltration of various immune cells. Kaplan-Meier survival analysis showed that LUAD related with a poor prognosis in terms of OS, PFI, and DSS compared with high-expression LASTR. The enrichment analysis showed that LASTR is related to the pathays like PI3K-AKT signaling pathway. Thus, the present findings could be helpful in a better understand of LASTR in LUAD. RT-PCR was used to verify the high expression of LASTR in LUAD tissues, and the apoptosis of LUAD cell lines was promoted by CCK8 and Transwell experiments to verify the ability of LASTR to promote the migration and invasion of lung cancer cells in vitro. PeerJ Inc. 2023-11-28 /pmc/articles/PMC10691363/ /pubmed/38047034 http://dx.doi.org/10.7717/peerj.16167 Text en ©2023 Kong et al. https://creativecommons.org/licenses/by/4.0/This is an open access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/) , which permits unrestricted use, distribution, reproduction and adaptation in any medium and for any purpose provided that it is properly attributed. For attribution, the original author(s), title, publication source (PeerJ) and either DOI or URL of the article must be cited. |
spellingShingle | Bioinformatics Kong, Fanming Yang, Xinyu Lu, Zhichao Liu, Zongheng Yang, Yang Wang, Ziheng A novel long noncoding RNA (lncRNA), LINC02657(LASTR), is a prognostic biomarker associated with immune infiltrates of lung adenocarcinoma based on unsupervised cluster analysis |
title | A novel long noncoding RNA (lncRNA), LINC02657(LASTR), is a prognostic biomarker associated with immune infiltrates of lung adenocarcinoma based on unsupervised cluster analysis |
title_full | A novel long noncoding RNA (lncRNA), LINC02657(LASTR), is a prognostic biomarker associated with immune infiltrates of lung adenocarcinoma based on unsupervised cluster analysis |
title_fullStr | A novel long noncoding RNA (lncRNA), LINC02657(LASTR), is a prognostic biomarker associated with immune infiltrates of lung adenocarcinoma based on unsupervised cluster analysis |
title_full_unstemmed | A novel long noncoding RNA (lncRNA), LINC02657(LASTR), is a prognostic biomarker associated with immune infiltrates of lung adenocarcinoma based on unsupervised cluster analysis |
title_short | A novel long noncoding RNA (lncRNA), LINC02657(LASTR), is a prognostic biomarker associated with immune infiltrates of lung adenocarcinoma based on unsupervised cluster analysis |
title_sort | novel long noncoding rna (lncrna), linc02657(lastr), is a prognostic biomarker associated with immune infiltrates of lung adenocarcinoma based on unsupervised cluster analysis |
topic | Bioinformatics |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10691363/ https://www.ncbi.nlm.nih.gov/pubmed/38047034 http://dx.doi.org/10.7717/peerj.16167 |
work_keys_str_mv | AT kongfanming anovellongnoncodingrnalncrnalinc02657lastrisaprognosticbiomarkerassociatedwithimmuneinfiltratesoflungadenocarcinomabasedonunsupervisedclusteranalysis AT yangxinyu anovellongnoncodingrnalncrnalinc02657lastrisaprognosticbiomarkerassociatedwithimmuneinfiltratesoflungadenocarcinomabasedonunsupervisedclusteranalysis AT luzhichao anovellongnoncodingrnalncrnalinc02657lastrisaprognosticbiomarkerassociatedwithimmuneinfiltratesoflungadenocarcinomabasedonunsupervisedclusteranalysis AT liuzongheng anovellongnoncodingrnalncrnalinc02657lastrisaprognosticbiomarkerassociatedwithimmuneinfiltratesoflungadenocarcinomabasedonunsupervisedclusteranalysis AT yangyang anovellongnoncodingrnalncrnalinc02657lastrisaprognosticbiomarkerassociatedwithimmuneinfiltratesoflungadenocarcinomabasedonunsupervisedclusteranalysis AT wangziheng anovellongnoncodingrnalncrnalinc02657lastrisaprognosticbiomarkerassociatedwithimmuneinfiltratesoflungadenocarcinomabasedonunsupervisedclusteranalysis AT kongfanming novellongnoncodingrnalncrnalinc02657lastrisaprognosticbiomarkerassociatedwithimmuneinfiltratesoflungadenocarcinomabasedonunsupervisedclusteranalysis AT yangxinyu novellongnoncodingrnalncrnalinc02657lastrisaprognosticbiomarkerassociatedwithimmuneinfiltratesoflungadenocarcinomabasedonunsupervisedclusteranalysis AT luzhichao novellongnoncodingrnalncrnalinc02657lastrisaprognosticbiomarkerassociatedwithimmuneinfiltratesoflungadenocarcinomabasedonunsupervisedclusteranalysis AT liuzongheng novellongnoncodingrnalncrnalinc02657lastrisaprognosticbiomarkerassociatedwithimmuneinfiltratesoflungadenocarcinomabasedonunsupervisedclusteranalysis AT yangyang novellongnoncodingrnalncrnalinc02657lastrisaprognosticbiomarkerassociatedwithimmuneinfiltratesoflungadenocarcinomabasedonunsupervisedclusteranalysis AT wangziheng novellongnoncodingrnalncrnalinc02657lastrisaprognosticbiomarkerassociatedwithimmuneinfiltratesoflungadenocarcinomabasedonunsupervisedclusteranalysis |