Cargando…

Transposon accumulation at xenobiotic gene family loci in aphids

The evolution of resistance is a major challenge for the sustainable control of pests and pathogens. Thus, a deeper understanding of the evolutionary and genomic mechanisms underpinning resistance evolution is required to safeguard health and food production. Several studies have implicated transpos...

Descripción completa

Detalles Bibliográficos
Autores principales: Baril, Tobias, Pym, Adam, Bass, Chris, Hayward, Alex
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Cold Spring Harbor Laboratory Press 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10691553/
https://www.ncbi.nlm.nih.gov/pubmed/37852781
http://dx.doi.org/10.1101/gr.277820.123
_version_ 1785152757722775552
author Baril, Tobias
Pym, Adam
Bass, Chris
Hayward, Alex
author_facet Baril, Tobias
Pym, Adam
Bass, Chris
Hayward, Alex
author_sort Baril, Tobias
collection PubMed
description The evolution of resistance is a major challenge for the sustainable control of pests and pathogens. Thus, a deeper understanding of the evolutionary and genomic mechanisms underpinning resistance evolution is required to safeguard health and food production. Several studies have implicated transposable elements (TEs) in xenobiotic-resistance evolution in insects. However, analyses are generally restricted to one insect species and/or one or a few xenobiotic gene families (XGFs). We examine evidence for TE accumulation at XGFs by performing a comparative genomic analysis across 20 aphid genomes, considering major subsets of XGFs involved in metabolic resistance to insecticides: cytochrome P450s, glutathione S-transferases, esterases, UDP-glucuronosyltransferases, and ABC transporters. We find that TEs are significantly enriched at XGFs compared with other genes. XGFs show similar levels of TE enrichment to those of housekeeping genes. But unlike housekeeping genes, XGFs are not constitutively expressed in germline cells, supporting the selective enrichment of TEs at XGFs rather than enrichment owing to chromatin availability. Hotspots of extreme TE enrichment occur around certain XGFs. We find, in aphids of agricultural importance, particular enrichment of TEs around cytochrome P450 genes with known functions in the detoxification of synthetic insecticides. Our results provide evidence supporting a general role for TEs as a source of genomic variation at host XGFs and highlight the existence of considerable variability in TE content across XGFs and host species. These findings show the need for detailed functional verification analyses to clarify the significance of individual TE insertions and elucidate underlying mechanisms at TE–XGF hotspots.
format Online
Article
Text
id pubmed-10691553
institution National Center for Biotechnology Information
language English
publishDate 2023
publisher Cold Spring Harbor Laboratory Press
record_format MEDLINE/PubMed
spelling pubmed-106915532023-12-02 Transposon accumulation at xenobiotic gene family loci in aphids Baril, Tobias Pym, Adam Bass, Chris Hayward, Alex Genome Res Research The evolution of resistance is a major challenge for the sustainable control of pests and pathogens. Thus, a deeper understanding of the evolutionary and genomic mechanisms underpinning resistance evolution is required to safeguard health and food production. Several studies have implicated transposable elements (TEs) in xenobiotic-resistance evolution in insects. However, analyses are generally restricted to one insect species and/or one or a few xenobiotic gene families (XGFs). We examine evidence for TE accumulation at XGFs by performing a comparative genomic analysis across 20 aphid genomes, considering major subsets of XGFs involved in metabolic resistance to insecticides: cytochrome P450s, glutathione S-transferases, esterases, UDP-glucuronosyltransferases, and ABC transporters. We find that TEs are significantly enriched at XGFs compared with other genes. XGFs show similar levels of TE enrichment to those of housekeeping genes. But unlike housekeeping genes, XGFs are not constitutively expressed in germline cells, supporting the selective enrichment of TEs at XGFs rather than enrichment owing to chromatin availability. Hotspots of extreme TE enrichment occur around certain XGFs. We find, in aphids of agricultural importance, particular enrichment of TEs around cytochrome P450 genes with known functions in the detoxification of synthetic insecticides. Our results provide evidence supporting a general role for TEs as a source of genomic variation at host XGFs and highlight the existence of considerable variability in TE content across XGFs and host species. These findings show the need for detailed functional verification analyses to clarify the significance of individual TE insertions and elucidate underlying mechanisms at TE–XGF hotspots. Cold Spring Harbor Laboratory Press 2023-10 /pmc/articles/PMC10691553/ /pubmed/37852781 http://dx.doi.org/10.1101/gr.277820.123 Text en © 2023 Baril et al.; Published by Cold Spring Harbor Laboratory Press https://creativecommons.org/licenses/by/4.0/This article, published in Genome Research, is available under a Creative Commons License (Attribution 4.0 International), as described at http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) .
spellingShingle Research
Baril, Tobias
Pym, Adam
Bass, Chris
Hayward, Alex
Transposon accumulation at xenobiotic gene family loci in aphids
title Transposon accumulation at xenobiotic gene family loci in aphids
title_full Transposon accumulation at xenobiotic gene family loci in aphids
title_fullStr Transposon accumulation at xenobiotic gene family loci in aphids
title_full_unstemmed Transposon accumulation at xenobiotic gene family loci in aphids
title_short Transposon accumulation at xenobiotic gene family loci in aphids
title_sort transposon accumulation at xenobiotic gene family loci in aphids
topic Research
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10691553/
https://www.ncbi.nlm.nih.gov/pubmed/37852781
http://dx.doi.org/10.1101/gr.277820.123
work_keys_str_mv AT bariltobias transposonaccumulationatxenobioticgenefamilylociinaphids
AT pymadam transposonaccumulationatxenobioticgenefamilylociinaphids
AT basschris transposonaccumulationatxenobioticgenefamilylociinaphids
AT haywardalex transposonaccumulationatxenobioticgenefamilylociinaphids