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Survey of the infant male urobiome and genomic analysis of Actinotignum spp.
The urinary bladder harbors a community of microbes termed the urobiome, which remains understudied. In this study, we present the urobiome of healthy infant males from samples collected by transurethral catheterization. Using a combination of enhanced culture and amplicon sequencing, we identify se...
Autores principales: | , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Nature Publishing Group UK
2023
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10692110/ https://www.ncbi.nlm.nih.gov/pubmed/38040700 http://dx.doi.org/10.1038/s41522-023-00457-6 |
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author | Reasoner, Seth A. Flores, Viktor Van Horn, Gerald Morales, Grace Peard, Leslie M. Abelson, Benjamin Manuel, Carmila Lee, Jessica Baker, Bailey Williams, Timothy Schmitz, Jonathan E. Clayton, Douglass B. Hadjifrangiskou, Maria |
author_facet | Reasoner, Seth A. Flores, Viktor Van Horn, Gerald Morales, Grace Peard, Leslie M. Abelson, Benjamin Manuel, Carmila Lee, Jessica Baker, Bailey Williams, Timothy Schmitz, Jonathan E. Clayton, Douglass B. Hadjifrangiskou, Maria |
author_sort | Reasoner, Seth A. |
collection | PubMed |
description | The urinary bladder harbors a community of microbes termed the urobiome, which remains understudied. In this study, we present the urobiome of healthy infant males from samples collected by transurethral catheterization. Using a combination of enhanced culture and amplicon sequencing, we identify several common bacterial genera that can be further investigated for their effects on urinary health across the lifespan. Many genera were shared between all samples suggesting a consistent urobiome composition among this cohort. We note that, for this cohort, early life exposures including mode of birth (vaginal vs. Cesarean section), or prior antibiotic exposure did not influence urobiome composition. In addition, we report the isolation of culturable bacteria from the bladders of these infant males, including Actinotignum spp., a bacterial genus that has been associated with urinary tract infections in older male adults. Herein, we isolate and sequence 9 distinct strains of Actinotignum spp. enhancing the genomic knowledge surrounding this genus and opening avenues for delineating the microbiology of this urobiome constituent. Furthermore, we present a framework for using the combination of culture-dependent and sequencing methodologies for uncovering mechanisms in the urobiome. |
format | Online Article Text |
id | pubmed-10692110 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2023 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-106921102023-12-03 Survey of the infant male urobiome and genomic analysis of Actinotignum spp. Reasoner, Seth A. Flores, Viktor Van Horn, Gerald Morales, Grace Peard, Leslie M. Abelson, Benjamin Manuel, Carmila Lee, Jessica Baker, Bailey Williams, Timothy Schmitz, Jonathan E. Clayton, Douglass B. Hadjifrangiskou, Maria NPJ Biofilms Microbiomes Article The urinary bladder harbors a community of microbes termed the urobiome, which remains understudied. In this study, we present the urobiome of healthy infant males from samples collected by transurethral catheterization. Using a combination of enhanced culture and amplicon sequencing, we identify several common bacterial genera that can be further investigated for their effects on urinary health across the lifespan. Many genera were shared between all samples suggesting a consistent urobiome composition among this cohort. We note that, for this cohort, early life exposures including mode of birth (vaginal vs. Cesarean section), or prior antibiotic exposure did not influence urobiome composition. In addition, we report the isolation of culturable bacteria from the bladders of these infant males, including Actinotignum spp., a bacterial genus that has been associated with urinary tract infections in older male adults. Herein, we isolate and sequence 9 distinct strains of Actinotignum spp. enhancing the genomic knowledge surrounding this genus and opening avenues for delineating the microbiology of this urobiome constituent. Furthermore, we present a framework for using the combination of culture-dependent and sequencing methodologies for uncovering mechanisms in the urobiome. Nature Publishing Group UK 2023-12-01 /pmc/articles/PMC10692110/ /pubmed/38040700 http://dx.doi.org/10.1038/s41522-023-00457-6 Text en © The Author(s) 2023 https://creativecommons.org/licenses/by/4.0/Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/ (https://creativecommons.org/licenses/by/4.0/) . |
spellingShingle | Article Reasoner, Seth A. Flores, Viktor Van Horn, Gerald Morales, Grace Peard, Leslie M. Abelson, Benjamin Manuel, Carmila Lee, Jessica Baker, Bailey Williams, Timothy Schmitz, Jonathan E. Clayton, Douglass B. Hadjifrangiskou, Maria Survey of the infant male urobiome and genomic analysis of Actinotignum spp. |
title | Survey of the infant male urobiome and genomic analysis of Actinotignum spp. |
title_full | Survey of the infant male urobiome and genomic analysis of Actinotignum spp. |
title_fullStr | Survey of the infant male urobiome and genomic analysis of Actinotignum spp. |
title_full_unstemmed | Survey of the infant male urobiome and genomic analysis of Actinotignum spp. |
title_short | Survey of the infant male urobiome and genomic analysis of Actinotignum spp. |
title_sort | survey of the infant male urobiome and genomic analysis of actinotignum spp. |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10692110/ https://www.ncbi.nlm.nih.gov/pubmed/38040700 http://dx.doi.org/10.1038/s41522-023-00457-6 |
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