Characterization of a thermostable Cas12a ortholog

CRISPR-Cas12a has been used for genome editing and molecular diagnosis. The well-studied Cas12a orthologs have a T-rich PAM and are usually categorized as non-thermally stable enzymes. Here, we identified a new Cas12a ortholog from Clostridium thermobutyricum, which survives at 60 °C. This Cas12a or...

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Detalles Bibliográficos
Autores principales: Wu, Jing, Gao, Pan, Shi, Yajing, Zhang, Caixiang, Tong, Xiaohan, Fan, Huidi, Zhou, Xi, Zhang, Ying, Yin, Hao
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Research Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10692460/
https://www.ncbi.nlm.nih.gov/pubmed/38047138
http://dx.doi.org/10.1016/j.cellin.2023.100126

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