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MiR-548c-3p through suppressing Tyms and Abcg2 increases the sensitivity of colorectal cancer cells to 5-fluorouracil

BACKGROUND: Colorectal cancer, is one of most prevalent the cancer in the world. 5-Fluorouracil is a standard chemotherapeutic drug while the acquisition of resistance to 5-Fluorouracil is one of the problems during treatment. In this study, we aimed to find the miRNAs that modulate the expression o...

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Autores principales: Khalili, Elham, Afgar, Ali, Rajabpour, Azam, Aghaee-Bakhtiari, Seyed Hamid, Jamialahmadi, Khadijeh, Teimoori-Toolabi, Ladan
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2023
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10692789/
https://www.ncbi.nlm.nih.gov/pubmed/38045156
http://dx.doi.org/10.1016/j.heliyon.2023.e21775
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author Khalili, Elham
Afgar, Ali
Rajabpour, Azam
Aghaee-Bakhtiari, Seyed Hamid
Jamialahmadi, Khadijeh
Teimoori-Toolabi, Ladan
author_facet Khalili, Elham
Afgar, Ali
Rajabpour, Azam
Aghaee-Bakhtiari, Seyed Hamid
Jamialahmadi, Khadijeh
Teimoori-Toolabi, Ladan
author_sort Khalili, Elham
collection PubMed
description BACKGROUND: Colorectal cancer, is one of most prevalent the cancer in the world. 5-Fluorouracil is a standard chemotherapeutic drug while the acquisition of resistance to 5-Fluorouracil is one of the problems during treatment. In this study, we aimed to find the miRNAs that modulate the expression of Tyms and Abcg2 as resistance-inducing genes in the resistant cell lines to 5-Fluorouracil. METHODS: 5-Fluorouracil-resistant HCT116 and SW480 cell lines were generated by consecutive treatment of cells with 5-Fluorouracil. This resistance induction was validated by MTT assays. The expression of the Tyms and Abcg2 gene and miR-548c-3p were studied by quantitative real-time PCR in the cell lines. RESULTS: We hypothesized that miR-548c-3p is targeting Tyms and Abcg2 simultaneously. Increased expression Tyms gene in the two most resistant cell lines derived from HCT116 and all resistant cell lines derived from SW480 except one were seen. Increased expression of Abcg2 was observed in the most resistant HCT116-derived cell line and all resistant cell lines, derived from SW480. In all resistant cell lines, the expression of miR-548c-3p was decreased. CONCLUSION: It can be concluded downregulation of miR548c-3p is in line with Tyms and Abcg2 overexpression in resistant cell lines to 5-Fluorouracil.
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spelling pubmed-106927892023-12-03 MiR-548c-3p through suppressing Tyms and Abcg2 increases the sensitivity of colorectal cancer cells to 5-fluorouracil Khalili, Elham Afgar, Ali Rajabpour, Azam Aghaee-Bakhtiari, Seyed Hamid Jamialahmadi, Khadijeh Teimoori-Toolabi, Ladan Heliyon Research Article BACKGROUND: Colorectal cancer, is one of most prevalent the cancer in the world. 5-Fluorouracil is a standard chemotherapeutic drug while the acquisition of resistance to 5-Fluorouracil is one of the problems during treatment. In this study, we aimed to find the miRNAs that modulate the expression of Tyms and Abcg2 as resistance-inducing genes in the resistant cell lines to 5-Fluorouracil. METHODS: 5-Fluorouracil-resistant HCT116 and SW480 cell lines were generated by consecutive treatment of cells with 5-Fluorouracil. This resistance induction was validated by MTT assays. The expression of the Tyms and Abcg2 gene and miR-548c-3p were studied by quantitative real-time PCR in the cell lines. RESULTS: We hypothesized that miR-548c-3p is targeting Tyms and Abcg2 simultaneously. Increased expression Tyms gene in the two most resistant cell lines derived from HCT116 and all resistant cell lines derived from SW480 except one were seen. Increased expression of Abcg2 was observed in the most resistant HCT116-derived cell line and all resistant cell lines, derived from SW480. In all resistant cell lines, the expression of miR-548c-3p was decreased. CONCLUSION: It can be concluded downregulation of miR548c-3p is in line with Tyms and Abcg2 overexpression in resistant cell lines to 5-Fluorouracil. Elsevier 2023-11-05 /pmc/articles/PMC10692789/ /pubmed/38045156 http://dx.doi.org/10.1016/j.heliyon.2023.e21775 Text en © 2023 The Authors. Published by Elsevier Ltd. https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
spellingShingle Research Article
Khalili, Elham
Afgar, Ali
Rajabpour, Azam
Aghaee-Bakhtiari, Seyed Hamid
Jamialahmadi, Khadijeh
Teimoori-Toolabi, Ladan
MiR-548c-3p through suppressing Tyms and Abcg2 increases the sensitivity of colorectal cancer cells to 5-fluorouracil
title MiR-548c-3p through suppressing Tyms and Abcg2 increases the sensitivity of colorectal cancer cells to 5-fluorouracil
title_full MiR-548c-3p through suppressing Tyms and Abcg2 increases the sensitivity of colorectal cancer cells to 5-fluorouracil
title_fullStr MiR-548c-3p through suppressing Tyms and Abcg2 increases the sensitivity of colorectal cancer cells to 5-fluorouracil
title_full_unstemmed MiR-548c-3p through suppressing Tyms and Abcg2 increases the sensitivity of colorectal cancer cells to 5-fluorouracil
title_short MiR-548c-3p through suppressing Tyms and Abcg2 increases the sensitivity of colorectal cancer cells to 5-fluorouracil
title_sort mir-548c-3p through suppressing tyms and abcg2 increases the sensitivity of colorectal cancer cells to 5-fluorouracil
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10692789/
https://www.ncbi.nlm.nih.gov/pubmed/38045156
http://dx.doi.org/10.1016/j.heliyon.2023.e21775
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