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Protocol for in vitro sonoporation validation using non-targeted microbubbles for human studies of ultrasound-mediated gene delivery
Microbubbles are currently approved for diagnostic ultrasound imaging and are under evaluation in therapeutic protocols. Here, we present a protocol for in vitro sonoporation validation using non-targeted microbubbles for gene delivery. We describe steps for computational simulation, experimental ca...
| Autores principales: | , , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Elsevier
2023
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10692958/ https://www.ncbi.nlm.nih.gov/pubmed/37976155 http://dx.doi.org/10.1016/j.xpro.2023.102723 |
| _version_ | 1785153051281063936 |
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| author | Zhang, Nisi Guo, Yutong Foiret, Josquin Tumbale, Spencer K. Paulmurugan, Ramasamy Ferrara, Katherine W. |
| author_facet | Zhang, Nisi Guo, Yutong Foiret, Josquin Tumbale, Spencer K. Paulmurugan, Ramasamy Ferrara, Katherine W. |
| author_sort | Zhang, Nisi |
| collection | PubMed |
| description | Microbubbles are currently approved for diagnostic ultrasound imaging and are under evaluation in therapeutic protocols. Here, we present a protocol for in vitro sonoporation validation using non-targeted microbubbles for gene delivery. We describe steps for computational simulation, experimental calibration, reagent preparation, ultrasound treatment, validation, and gene expression analysis. This protocol uses approved diagnostic microbubbles and parameters that are applicable for human use. For complete details on the use and execution of this protocol, please refer to Bez et al. (2017).(1) |
| format | Online Article Text |
| id | pubmed-10692958 |
| institution | National Center for Biotechnology Information |
| language | English |
| publishDate | 2023 |
| publisher | Elsevier |
| record_format | MEDLINE/PubMed |
| spelling | pubmed-106929582023-12-03 Protocol for in vitro sonoporation validation using non-targeted microbubbles for human studies of ultrasound-mediated gene delivery Zhang, Nisi Guo, Yutong Foiret, Josquin Tumbale, Spencer K. Paulmurugan, Ramasamy Ferrara, Katherine W. STAR Protoc Protocol Microbubbles are currently approved for diagnostic ultrasound imaging and are under evaluation in therapeutic protocols. Here, we present a protocol for in vitro sonoporation validation using non-targeted microbubbles for gene delivery. We describe steps for computational simulation, experimental calibration, reagent preparation, ultrasound treatment, validation, and gene expression analysis. This protocol uses approved diagnostic microbubbles and parameters that are applicable for human use. For complete details on the use and execution of this protocol, please refer to Bez et al. (2017).(1) Elsevier 2023-11-16 /pmc/articles/PMC10692958/ /pubmed/37976155 http://dx.doi.org/10.1016/j.xpro.2023.102723 Text en © 2023 The Authors https://creativecommons.org/licenses/by-nc-nd/4.0/This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/). |
| spellingShingle | Protocol Zhang, Nisi Guo, Yutong Foiret, Josquin Tumbale, Spencer K. Paulmurugan, Ramasamy Ferrara, Katherine W. Protocol for in vitro sonoporation validation using non-targeted microbubbles for human studies of ultrasound-mediated gene delivery |
| title | Protocol for in vitro sonoporation validation using non-targeted microbubbles for human studies of ultrasound-mediated gene delivery |
| title_full | Protocol for in vitro sonoporation validation using non-targeted microbubbles for human studies of ultrasound-mediated gene delivery |
| title_fullStr | Protocol for in vitro sonoporation validation using non-targeted microbubbles for human studies of ultrasound-mediated gene delivery |
| title_full_unstemmed | Protocol for in vitro sonoporation validation using non-targeted microbubbles for human studies of ultrasound-mediated gene delivery |
| title_short | Protocol for in vitro sonoporation validation using non-targeted microbubbles for human studies of ultrasound-mediated gene delivery |
| title_sort | protocol for in vitro sonoporation validation using non-targeted microbubbles for human studies of ultrasound-mediated gene delivery |
| topic | Protocol |
| url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC10692958/ https://www.ncbi.nlm.nih.gov/pubmed/37976155 http://dx.doi.org/10.1016/j.xpro.2023.102723 |
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